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Response of mungbeen germplasm against collar rot disease, caused by Phytophthora Megasperma and its chemical management

Research Paper | March 1, 2018

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Muhammad Kamran, Muhammad Ehetisham Ul Haq, Shaukat Ali, Muhammad Idrees, Muhammad Iqbal, Huma Abbas, Muhammad Azhar Iqbal, Muhammad Rafiq, Husnain Abbas, Nasir Subhani

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Int. J. Biosci.12( 3), 49-55, March 2018

DOI: http://dx.doi.org/10.12692/ijb/12.3.49-55


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Mungbean (Vigna radiata) is grown mainly for its edible seeds. Collar rot disease caused by Phytophthora megasperma is a serious limiting factor for its cultivation. The current research was planned to evaluate the response of eighty mungbean germplasm against collar rot disease. These germplasm were sown in sick plot in augmented design with 10 cm plant to plant and 30 cm row to row distance at research area of Plant Pathology Research Institute (PPRI), Ayub Agricultural Research Institute (AARI), Faisalabad, Pakistan. Check varietywas sown after every five entries. Data was recorded on plant mortality basis according to disease rating scale. No germplasm was found immune. Eight germplasm exhibited highly resistant while 15 germplasm showed resistance response against the disease. Nineteen germplasm were moderately resistant. Twenty-seven germplasm were found susceptible and eleven were highly susceptible with more than 50% plant mortality.Then efficacy of different fungicides was evaluated against P. megaspermain vitro. Culture of P. megasperma was isolated from infected mungbean samples on PARP media [Pimaricin, Ampicillin, Rifampicin, Pentachloronitrobenzene (PCNB)].  For evaluation of fungicides Potato Dextrose Agar (PDA) amended with different fungicides at different concentrations (10, 50, 100 and 200 ppm) was used. Experiment was conducted in completely randomized design with fifteen replications. Data was recorded on mycelial growth (mm) of fungus. Ridomil Gold (Mancozeb + Metalaxyl-M) significantly inhibited the growth at all the concentrations. After this Revus (mandipropamid), Acrobat (Dimethomorph) and Amistar Top (azoxystrobin + difenoconazole) inhibited the growth as compared to control respectively. Score (difenconazole) was significantly least effective as it even at 200 ppm exhibited 50.8 (mm) mucelial growth of P. megasperma. The current research is useful to find out resistance source of mungbean germplasm against collar rot disease and its chemical management.


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Response of mungbeen germplasm against collar rot disease, caused by Phytophthora Megasperma and its chemical management

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