Review on use of recent molecular techniques to access biodiversity in plants

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Review Paper 01/10/2019
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Review on use of recent molecular techniques to access biodiversity in plants

Shahid Ali Chand, Muhammad Zohaib Hassan, Umair Rasool Azmi, Muhammad Ilyas, Iqra Kanwal, Ghulam Mujtaba Atif, Aqdas Hameed, Atif Haroon, Tayyaba Yasin, Muhammad Huzaifa Mahmood, Muhammad Yousof Zahoor, Muhammad Nabeel Aslam
J. Bio. Env. Sci.15( 4), 100-107, October 2019.
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Molecular tools developed in the past few years provide easy, less laborious means for assigning known and unknown plant taxa. These techniques answer many new evolutionary and taxonomic questions, which were not previously possible with only phenotypic methods. Molecular techniques such as DNA barcoding, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), microsatellites and single nucleotide polymorphisms (SNP) have recently been used for plant diversity studies. This review presents a basic description of different molecular techniques that can be utilized for DNA fingerprinting and molecular diversity analysis of plant species. DNA barcoding uses particular regions of DNA making helping in categorization and recognize unknown species. Researchers now interested to generate DNA barcodes designed for all living organisms and to build up data accessible to public to help in understanding of natural biodiversity of world. Cyclotides are peptides derived from plants with particular head to tail cyclic backbone that have three disulphide bonds by forming a cystine knot. Recent information about DNA barcoding can be used for detection of unidentified biological specimens to a taxonomic group, accurate detection of phytomedicinals, and in the biodiversity of living organisms.


Ahmad I, Khan S, Naeem M, Hayat M, Azmi UR, Ahmed S, Murtaza G, Irfan M. 2019. Molecular Identification of Ten Palm Species using DNA Fingerprinting, Int. J. Pure App. Biosci 7(1), 46-51.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J. Mol. Biol 215, 403-410.

Armstrong KF, Ball SL. 2005. DNA barcodes for biosecurity: invasive species identification. Philos Trans R Soc B BiolSci 360,1813-1823.

Baldwin BG.1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. Mol. Phylogenet. Evol 1, 3-16.

Coissac E, Hollingsworth PM, Lavergne S, Taberlet P. 2016. From barcodes to genomes: extending the concept of DNA barcoding. Molecular Ecology 25, 1423-1428.

Costion CM, Kress WJ, Crayn DM. 2016. DNA barcodes confirm the taxonomic and conservation status of a species of tree on the brink of extinction in the Pacific. PLOS ONE 11, e0155118.

Craik DJ. 2015. Plant cyclotides. Advances in Botanical Research, Volume 76, London: Academic Press.

Erickson DL, Jones FA, Swenson NG, Pei N, Bourg N, Chen W, Davies SJ, Ge X, Hao Z, Howe RW, Huang CL, Larson A, Lum S, Lutz JA, Ma K, Meegaskumbura M, Mi X, Parker JD, Fang Sun I, Wright J, Wolf AT, Ye W, Xing D, Zimmerman JK, Kress WJ. 2014. Comparative evolutionary diversity and phylogenetic structure across multiple forest dynamics plots: A megaphy logeny approach. Frontiers in Genetics 5, 358.

Hamilton JP, Buell CR. 2012. Advances in plant genome sequencing. Plant J, 70, 177-190.

Hebert PD, Cywinska A, Ball SL, de Waard JR. 2003. Biological identifications through DNA barcodes. Proc Bio lSci, 270, 313-321.

Kress WJ, Erickson DL, Jones FA, Swenson NG, Perez R, Sanjur O, Bermingham E. 2009. Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proceedings of the National Academy of Sciences USA 106, 1862-18626.

Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH. 2005. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences USA 102, 8369-8374.

Kress WJ. 2011. DNA barcoding and systematic monographs. In: T Stuessy, W Lack eds. Monographic plant systematics: Fundamental assessment of plant biodiversity. Berlin: Regnum Vegetabile 153. A.R. G. Gantner Verlag K.G. 49-71.

Lahaye R, Bank M, Bogarin D. 2008. DNA barcoding the floras of biodiversity hotspots. Proc Natl Acad Sci USA, 105, 2923-2928.

Liu YJ, Newmaster SG, Wu XJ, Liu Y, Ragupathy S, Motley T, Long CL. 2013. Pinelliahunanensis (Araceae), a new species supported by morphometric analysis and DNA barcoding. Phytotaxa 130, 1-13.

Newmaster SG, Fazekas AJ, Steeves RAD, Janovec J. 2008. Testing candidate plant barcode regions in the Myristicaceae.Molecular Ecology Resources 8, 480-490.

Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S. 2013. DNA barcoding detects contamination and substitution in North American herbal products. BMC Medicine 11, 222.

Nicole S, Negrisolo E, Eccher G, Mantovani R, Patarnello T, Erickson DL, Kress WJ, Barcaccia G. 2012. DNA barcoding as a reliable method for the authentication of commercial seafood products. Food Technology and Biotechnology 50, 387-398.

Olsvik O, Wahlberg J, Petterson B, Uhlén M, Popovic T, Wachsmuth IK, Fields PI. 1993. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. J. Clin. Microbiol. 31, 22-25.

Schuster SC. 2008. Next-generation sequencing transforms today’s biology. Nat. Methods 5,16-18.

Shafiq S, Adeel M, Raza H, Iqbal R, Ahmad Z, Naeem M, Sheraz M, Ahmed U, Azmi UR. 2019. Effects of Foliar Application of Selenium in Maize (Zea Mays L.) under Cadmium Toxicity. Biological Forum-An International Journal 11(2), 27-3.

Swenson NG. 2012. Phylogenetic analyses of ecological communities using DNA barcode data. In: WJ Kress, DL Erickson eds. DNA barcodes: Methods and protocols. New York: Humana Press, Springer Science+Publishing Media, LLC. 409-419.

Swenson NG. 2013. The assembly of tropical tree communities the advances and shortcomings of phylogenetic and functional trait analyses. Ecography 36, 264-276.

Techen N, Parveen I, Pan Z, Khan IA. 2014. DNA barcoding of medicinal plant material for identification. Curr Opin Biotechnol 25, 103-110.

Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. 1995. AFLP: A new technique for DNA fingerprinting. Nucl. Acids Res 23, 4407-4414.

Webb CO, Donoghue MJ. 2005. Phylomatic: Tree assembly for applied phylogenetics. Molecular Ecology Notes 5, 181-183.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531-6535.