Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran
Paper Details
Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran
Abstract
Bunium persicum (Boiss) Fedtsch is a valuable medicinal plant that is facing extinction. A study was conducted to adopt various strategies and techniques to conserve and protect the biodiversity of B. persicum. Application of corm explant from six-month-old seedling on MS medium supplemented with various auxins showed that corm derived callus on MS medium supplemented with 1.0mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D) by 74.9% induced somatic embryogenesis callus. The somatic embryos transferred to medium supplemented with different concentrations of benzylaminopurine (BAP), kinetin, spermidine, forchlorfenuron (CPPU), chlormequat chloride (CCC), paclobutrazol (PBZ), casein hydrolysate (CH), poly ethylene glycol (PEG) and banana powder, led to maximum plantlet regeneration, which was 65.8 ± 2.6 obtained in ½ MS medium supplemented with 20g/l banana powder. Consequently, induction of somatic embryogenesis under 1.0mg/L 2, 4-D was found to be more suitable than other auxins and capacity of banana powder for plantlet development by having indol acetic acid (IAA), cytokinins and gibberellins (GAs) was more than other additives and PGRs. Also, the effect of different concentrations of sucrose, BAP, PBZ and GA3 on size of B. persicum corms was investigated. Results showed that 90 g/L sucrose with 164.9 ± 2.8 mg corm fresh weight (FW) was the most suitable sucrose concentration for growth of corm and shoot numbers. The corms coated with sodium alginate and calcium nitrate to create artificial seeds. The seeds transferred to soil and produced seeds in the first year.
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Yousef Emamipoor (2018), Role of biotechnology in protection of endangered medicinal plant, Bunium persicum; the most important herb of Iran; JBES, V12, N5, May, P311-323
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