Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | September 1, 2015

| Download 1

Role of new novel entomopathogenic fungi Paecilomyces lilacinus to mortality and infection process of Tetrancyhus kanzawai (Kishida) (Tetranychidae: Acarina)

Yayan Sanjaya, Virginia R. Ocampo, Barbara L. Caoili

Key Words:

Int. J. Biosci.7(3), 16-23, September 2015

DOI: http://dx.doi.org/10.12692/ijb/7.3.16-23


IJB 2015 [Generate Certificate]


P. lilacinus is a common soil hyphomycete with a cosmopolitan distribution. Since Its characteristic has similarity with Metarhizium and Beauveria, it is assumed that it can be effective as well Metarhizium and Beauveria . The experiment was conducted in the Green house with papaya plant. The papaya seedlings were hand-sprayed with suspension with a density of 1 x 106, 1 x 107, 1 x 108 conidia ml-1, with 3 replications. Adult mortality of Tetranycus kanzawai was observed daily with 10 x hand magnifying glass until 10 days of 100% mortality was reached (based on preliminary research), after treatment. Second experiment Scanning Electron Microscope (SEM) to know charactreistic of P.lilacinus and indection cause by its fungi.The third experimet was morphological characterization of fungi. The observations covered color and mycelia structure. Furthermore shape, conidia, and conidiophores were observed under binocular microscope and microcamera under 1000 x magnification from a small amount of colony taken from the flask. The result showed that on the concentration of 107 it cause mortality of T. kanzawai 70 %, while on 108 was 74,44%. The P.lilacinus characteristic that it has pink color, The color of young P. lilacinus colony was white, but when sporulating it changed to various shades of vinaceous. P. lilacinus formed a dense mycelium, bore phialides from the ends of which spores were formed in long chains. The reverse side is suncolored. Conidia were in divergent chains, ellipsoid to fusiform in shape, and smooth-walled to slightly roughened.The infection proses showed Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation and formed conidial germination Penetrations forming appressoria were also obserbed in P. lilacinus , characterized by a thickening of the extremity of the germ-tubes as well as extrusion and conidiogenesis which were almost similar with M. anisopliae and P. lilacinus.Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation , and formed conidial germination (Figure 3b). Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation (Figure 3a), and formed conidial germination.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Role of new novel entomopathogenic fungi Paecilomyces lilacinus to mortality and infection process of Tetrancyhus kanzawai (Kishida) (Tetranychidae: Acarina)

Abbott WS. 1925. A method for computing the effectiveness of an insecticide. J Econ Ent 18, 265-267.

Barrett OW. 1914. A fungus parasite of the coconut beetle. Philipp Agric 7, 133.

Bielikov Á, Landa ZZ, Lance OS, Curn V. 2002 Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique. Plant Protection Science 38(1), 1–12.

Boucias DG, Pendland JC. 1982. Ultrastructural studies on the fungus Nomuraea rileyi, infecting the velvet bean caterpillar, Anticarsia gemmatalis. J. Invertebr. Pathol 39, 338-345.

Bridge PD, Arora DK. 1998. PCR for species definition. In: BRIDGE P.D., ARORA D.K., REDDY C.A., ELANDER R.P. (eds.): Application of PCR in Mycology. CABI, Wallingford, 63–84.

Boucia DG, Pendland JC. 1998. Principles of Insect Pathology. New York: Academic Press.

Charnley AK. 1989: Mycoinsecticides – present use and future prospects. In: Progress and Prospects in Insect Control. BCPC Monograph No. 43, 165–181.

Chandler D, Hay D, Reid AP. 2000. Sampling and occurrence of entomopathogenicfungi and nematodes in UK soils. Applied Soil Ecology 5, 133– 141.

Cheng LL, Nechols JR, Margolies DC, Campbell JF, Yang PS, Chen CC, Lu CT. 2009. Foraging on and consumption of two species of papaya pest mites, Tetranychus kanzawai and Panonychus citri (Acari: Tetranychidae), by Mallada basalis (Neuroptera: Chrysopidae). Environ. Entomol 38(3), 715- 722 .

Dara SK, Hountondji FCC. 2001. Effects of Formulated Imidacloprid on Two Mite Pathogens, Neozygites floridana (Zygomycotina: Zygomycetes) and Hirsutella thompsonii (Deuteromycotina: Hyphomycetes). Insect Sci. Applic. 21(2), 133 -138.

Ferro P. 1972. Fungi as a microbial insecticide against pests. In: Arora, DK., Ajello, L., Mukerji, KG (Eds.), Handbook Appl. Mycol. 2, Humans, Animals, and Insects. Dekker, New York, 665-706.

Guavarra MR. 1981. The biology, ecology and control of spider mite Tetranychus kanzawai Kishida (Acarina: Tetranychida) on papaya. (PhD Thesis), College, Laguna, Philippines: University of the Philippines Los Banos. (Available at UPLB Main Library).

Pekru LS, Grula EA. 1979. Mode of infection of the corn earworm (Heliolithis zea) by Beauveria bassiana as revealed by scanning electron microscopy. Journal of Invertebrate Pathology (34), 238 – 247.

Roberts DW, Krasnoff SB. 1998. Toxins and enzymes of entomopathogenic fungi. In: ALVES, S.B. (Ed.) Microbial control of insects. 2. ed. Piracicaba: Fealq, 1998. Sec. 32, 967-985 P.

Steinhaus EA. 1963. Principle of Insect Pathology. Mcgraw-Hill, New York.

Leger STRJ. 1993. Biology and Mechanisms of Insect-Cuticle Invasion by Deuteromycete Fungal Pathogen, Parasites and Pathogens of Insect. New York : Academic Press.

Leger STRJ, Joshi RJL, Bidochka MJ, Roberts DW. Construction of an improved 1998 mycoinsecticide overexpressing a Toxicprotease. Proc. Natl. Acad. Sci. USA 93, 6349-6354, June 1996 Agricultural Sciences.

Leger STRJ, Joshi RJL, Roberts D. 1998. Ambient pH is a Major determinant in the expression of cuticle-degrading enzymes and hydrophobin by Metarhizium anisopliae. Appl Environ Microbiol, February 64(2), 709-713.

Leger STRJ, Nelson RJJO, Screen SE. 1999. The Entomopathogenic fungus Metarhizium anisopliae alters ambient pH, allowing extracellular protease production and activity. Microbiology 145, 2691–2699.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background