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Role of new novel entomopathogenic fungi Paecilomyces lilacinus to mortality and infection process of Tetrancyhus kanzawai (Kishida) (Tetranychidae: Acarina)

Yayan Sanjaya, Virginia R. Ocampo, Barbara L. Caoili

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Int. J. Biosci.7(3), 16-23, September 2015

DOI: http://dx.doi.org/10.12692/ijb/7.3.16-23

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Abstract

P. lilacinus is a common soil hyphomycete with a cosmopolitan distribution. Since Its characteristic has similarity with Metarhizium and Beauveria, it is assumed that it can be effective as well Metarhizium and Beauveria . The experiment was conducted in the Green house with papaya plant. The papaya seedlings were hand-sprayed with suspension with a density of 1 x 106, 1 x 107, 1 x 108 conidia ml-1, with 3 replications. Adult mortality of Tetranycus kanzawai was observed daily with 10 x hand magnifying glass until 10 days of 100% mortality was reached (based on preliminary research), after treatment. Second experiment Scanning Electron Microscope (SEM) to know charactreistic of P.lilacinus and indection cause by its fungi.The third experimet was morphological characterization of fungi. The observations covered color and mycelia structure. Furthermore shape, conidia, and conidiophores were observed under binocular microscope and microcamera under 1000 x magnification from a small amount of colony taken from the flask. The result showed that on the concentration of 107 it cause mortality of T. kanzawai 70 %, while on 108 was 74,44%. The P.lilacinus characteristic that it has pink color, The color of young P. lilacinus colony was white, but when sporulating it changed to various shades of vinaceous. P. lilacinus formed a dense mycelium, bore phialides from the ends of which spores were formed in long chains. The reverse side is suncolored. Conidia were in divergent chains, ellipsoid to fusiform in shape, and smooth-walled to slightly roughened.The infection proses showed Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation and formed conidial germination Penetrations forming appressoria were also obserbed in P. lilacinus , characterized by a thickening of the extremity of the germ-tubes as well as extrusion and conidiogenesis which were almost similar with M. anisopliae and P. lilacinus.Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation , and formed conidial germination (Figure 3b). Conidial adhesion to the integument happened immediately 12 to 24 hours after inoculation (Figure 3a), and formed conidial germination.

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Role of new novel entomopathogenic fungi Paecilomyces lilacinus to mortality and infection process of Tetrancyhus kanzawai (Kishida) (Tetranychidae: Acarina)

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