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Screening and evaluation of ligninolytic dye decolourisation capacity of Pleurotus ostreatus

Patient D. Dhliwayo-Chiunzi, Ruvimbo Rwafa, Lydia Mugayi, Fungai Siwanja, Talent Ngurube, Glasnost Hudson, Hope Mvundura, Shumirai Nyashonjeka, Tapiwaishe Madeya

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J. Bio. Env. Sci.6(3), 165-173, March 2015

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Abstract

Ligninolytic enzymes as biocatalysts have the potential to replace conventional processes in several industries including the water purification industry especially in the treatment of dye effluent. Development of water treatment systems based on ligninolytic enzymes is a desirable option because ligninolytic enzymes can degrade dyes of diverse chemical structure and be used in a wide variety of industries. Studies were carried out to evaluate and screen for ligninolytic dye decolourisation capacity of Pleurotus ostreatus (P.ostreatus). Pure fungal cultures of P.ostreatus were screened for ligninolytic enzyme activity using solid phase decolourisation of aromatic food dyes. The assay was carried out on PDA plates with 100mg/l of individual commercial food dyes – Sunset Yellow, Orange C10 and Lemon Yellow. All the food dyes were effectively bleached or decolourised by fungal mycelia of P.ostreatus after 10 days of active growth in the dark at 25oC. The observation strongly suggests the presence of fungal peroxidase enzymes which play a role in the degradation of synthetic lignin or dyes. Lignin peroxidase (LiP) activity from crude enzyme extract was determined using the method of Tien and Kirk, 1988. An average LiP activity of 7.635U/ml was observed under Solid State Fermentation (SSF) by P.ostreatus in wheat bran/soya bean substrate (90:10) after 10 days of full substrate colonisation. In agreement with the results of other workers, the study indicates potential for P.ostreatus for industrial production of ligninolytic enzymes through solid state fermentation on locally available agricultural products.

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Screening and evaluation of ligninolytic dye decolourisation capacity of Pleurotus ostreatus

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