Screening extracts of Asystasia vogeliana Benth. for anti-inflammatory potentials
Paper Details
Screening extracts of Asystasia vogeliana Benth. for anti-inflammatory potentials
Abstract
This study screened different parts of Asystasia vogeliana for anti-inflammatory potentials. Fresh A. vogeliana leaf, stem, and aerial parts were separately air-dried, pulverised, and extracted in 80% methanol (MeOH) for 72 hrs. Filtrates were separately concentrated to produce leaf, stem, and aerial extracts, respectively. Acute oral toxicity was determined on the leaf and stem extracts using OECD method. Then, extracts were screened for in vivo anti-inflammatory potentials via egg albumin-induced paw oedema model. The powdered leaf part was extracted with dichloromethane (DCM) and ethyl acetate (EtOAc) for 72 hrs and processed to produce DCM and EtOAc leaf extracts. Then, MeOH, DCM, and EtOAc leaf extracts were screened for in vitro anti-inflammatory activities via bovine erythrocyte membrane stabilisation, anti-denaturant, anti-tryptic, and nitric oxide scavenging assays. No mortality or sign of toxicity was observed at 2000 mg/kg. Compared with the untreated negative control, the various parts of A. vogeliana demonstrated ¬in vivo anti-inflammatory activities by reducing the paw oedema size at different time intervals. Most significant anti-inflammatory activity was obtained with the aerial extract. DCM leaf extract demonstrated the highest erythrocyte membrane stabilization (85.46±0.01%), anti-denaturant (89.52±0.01%), and NO scavenging (IC50 6.77±0.94 mg/ml) activities. MeOH leaf extract showed least in vitro anti-inflammatory activity. The study concludes that the stem and aerial parts of A. vogeliana are also a good source of anti-inflammatory compounds, in addition to the leaf part. No toxic effect was observed at 2000 mg/kg. Therefore, our findings substantiate the conventional use of A. vogeliana leaves as remedy for inflammatory-related conditions.
Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MN. 2018. The crucial roles of inflammatory mediators in inflammation: A review. Veterinary World 11(5), 627-635.
Achoui M, Appleton D, Abdulla, MA, Awang K, Mohd MA. 2010. In vitro and in vivo anti-inflammatory activities of 17-O-Acetylacuminolide through the inhibition of cytokines, NF-kB translocation and IKKβ activity. PlosOne 5(12), e15105.
Adeyemi OO, Aigbe FR, Uyaiabasi NG. 2011. Analgesic and anti-inflammatory activities of the aqueous stem and leaf extract of Asystasia gangetica (Linn) T. Anderson. Nigeria Quarterly Journal of Hospital Medicine 21(2), 129-134.
Akinpelu BA, Godwin Anyim, Aderogba MA, Makinde AM, Azeez SO, Oziegbe M. 2018. Evaluation of anti-inflammatory and genotoxicity potentials of the fractions of Archidium ohioense (Schimp. ex Mull) extract. Ife Journal of Science 20(3), 487-495.
Anosike CA, Odinaka NI, Nwodo OFC. 2019. Antioxidant properties and membrane stabilization effects of methanol extract of Mucuna pruriens leaves on normal and sickle erythrocytes. Journal of Traditional and Complementary Medicine 9(4), 278-284.
Bamidele AT, Oluwatobi O, Olusiji A, Kazeem AA, Idowu O, Funminiyi B, Adebayo O, Sandile S. 2017. Antimicrobial, antioxidant activities in vitro and polyphenol contents of the leaf extract of a versatile medicinal plant. Asian Journal of Applied Sciences 5(5), 01-11.
Bertram GK. 2004. Basic and Clinical Pharmacological. Lang medical book. McGraw-Hill (9th Ed). 298-582.
Boora F, Chirisa E, Mukanganyama S. 2014. Evaluation of nitrite radical scavenging properties of selected zimbabwean plant extracts and their phytoconstituents. Journal of Food Processing, 1-7.
Chen D, Holly S, Ana G, Tsutomu, A., Shamra M, Charlotte V, Chiao-Wen C, Xiaoqin F, Anne W, Wei B, Liang L, Lisa F, Kurt J, Yvonne RF. 2018. Inhibition of toll-like receptor-mediated inflammation in vitro and in vivo by a novel Benzoxaborole. The Journal of Pharmacology and Experimental Therapeutics 344, 436-446.
Farhood B, Mortezaee K, Goradel NH, Khanlarkhani N, Salehi E, Nashtaei MS, Najafi M, Sahebkar A. 2018. Curcumin as an anti‐inflammatory agent: implications to radiotherapy and chemotherapy. Journal of Cellular Physiology, 1‐13.
Gebhardt C, Hirschberger J, Rau S, Arndt G, Krainer K, Schweigert FJ, Kohn, B. 2009. Use of C-reactive protein to predict outcome in dogs with systemic inflammatory response syndrome or sepsis. J Vet. Emerg. Crit. Care 19, 450-458.
Gildas TM, Luis AV, Francisca GC, Otília DLP, Ana PRR. 2017. Reports on in vivo and in vitro contribution of medicinal plants to improve the female reproductive function. Reprodução and Climatério 32(2), 109-119.
Greaves MW. 1976. Anti-inflammatory action of corticosteroids. Postgraduate Medical Journal 52, 631-633.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. 1982. Analysis of nitrate, nitrite and (15N) nitrate in biological fluids. Analytical Biochemistry 126, 131-138.
Harborne JB. 1984. Phytochemical methods – a guide to modern techniques of plant analysis. 2nd ed. London: Chapman and Hall, 4–16.
Harirforoosh S, Asghar W, Jamali F. 2013. Adverse effects of nonsteroidal antiinflammatory drugs: an update of gastrointestinal, cardiovascular and renal complications. Pharm Pharm Sci 16(5), 821-847.
Hume DA, Underhill DM, Sweet MJ, Ozinsky AO, Liew FY. 2001 Macrophages exposed continuously to lipopolysaccharide and other agonists that act via toll-like receptors exhibit a sustained and additive activation state. BMC Immunol 2, 11
Iwalewa EO, McGaw LJ, Naidoo V, Eloff JN. 2007. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. African Journal of Biotechnology 6(25), 2868-2885.
Khan I, Sohai AJ, Zabta KS, Muhammad A, Yasmin K, Kumar T. 2017. Ethnobotany and medicinal uses of folklore medicinal plants belonging to family acanthaceae: An updated review. MOJ Biology and Medicine 1(2), 34‒38.
Komolafe NT. 2014. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea. An unpublished M. Sc. Thesis submitted to the Department of Life Science, University of South Africa. 50p.
Marrassini C, Peralta I, Claudia A. 2018. Comparative study of the polyphenol content‑related anti‑inflammatory and antioxidant activities of two Urera aurantiaca specimens from different geographical areas. Chinese Medicine 13(22), 2-12.
Marcocci L, Maguire JJ, Droylefaix MT, Packer L. 1994. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical Biophysical Research Communication 201, 748-755.
Medini F, Fellah H, Ksouri R, Abdelly C. 2014. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science, 1-10.
Miya GM, Oyemitan IA, Oyedeji OO, Oluwafemi SO, Nkeh-Chungage BN, Songca SP, Oyedeji AO. 2016. Phytochemical screening, anti-inflammatory and analgesic properties of Pentanisia prunelloides from the Eastern Cape province, South Africa. African Journal of Traditional, Complement and Alternative Medicine 13(6), 179-185.
OECD 2008. OECD guidelines for the testing of chemicals. 425, 1-25.
Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, Sipeolu FO. 2010. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry 2(4), 46-51.
Oyedapo OO, Famurewa AJ. 1995. Anti‐protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. International Journal of Pharmacognosy 33, 65‐69.
Oyemitan IA, Iwalewa EO, Akanmu MA, Olugbade TA. 2008. Antinociceptive and antiinflammatory effects of essential oil of Dennettia tripetala G. baker (annonaceae) in rodents. African Journal of Traditional, Complementary and Alternative Medicines 5(4), 355-362.
Popoola JO, Adebayo AH, Taiwo OS, Ayepola OO, Okosodo EF. 2017. Studies on Local Knowledge and In vitro Cytotoxicity of Moringa oleifera L., Andrographis paniculata N. and Asystasia vogeliana B. Extracts. Research Journal of Applied Sciences 12 (2), 180-190.
Praveena PE, Periasamy S, Kumar AA, Singh N. 2010. Cytokine profiles, apoptosis and pathology of experimental Pasteurella multocida serotype A1 infection in mice. Res. Vet. Sci 89, 332-339.
Puente XS, Lopez-Otin C. 2004. A genomic analysis of rat proteases and protease inhibitors. Genome Research 14 (4), 609-22.
Shalini S, Susmitha S, Ranganayaki P, Vijayaraghavan R. 2015. Evaluation of in vitro anti-inflammatory activity of aqueous extract of Andrographis paniculata. Global Journal of Pharmacology 9 (4), 289-295.
Shivraj HN, Park S. 2013. Optimized methods for in vitro and in vivo anti-inflammatory assays and its applications in herbal and synthetic drug analysis. Reviews in Medicinal Chemistry 13, 95-100.
Stankov SV. 2012. Definition of inflammation, causes of inflammation and possible anti-inflammatory strategies. The Open Inflammation Journal 5, 1-9.
Subathraa K, Poonguzhali V. 2012. In vitro studies on antioxidant and free radical scavenging activities of aqueous extract of Acorus calamus L. International Journal of Current Science and Technology, 169-173.
Tacham W. 2000. Plant master’s dissertation an ethnobotanical survey of plants used to treat diseases of the reproductive system in Foreke-Dschang and Fongo-Tongo in the Menoua division. Dschang-Cameroun: Université de Dschang, 22-4.
Tilloo, SK, Pande VB, Rasala TM, Kale VV. 2012. Asystasia gangetica: review on multi-potential application. International Research Journal of Pharmacy 3(4), 18-20.
Trease GE, Evans WC. 2002. Pharmacognosy (15th Edition) Saunders. 214-393 p.
Ugwuanyi HE, Aba PE, Samuel CU, Innocent IM. 2020. Acute toxicity and erythrocyte osmotic fragility studies of methanol leaf extract of Asystasia vogeliana in Rats. Journal of Applied Life Sciences International 23(2), 18-28.
Vignali DA, Kuchroo VK. 2012. IL-12 family cytokines: immunological playmakers. Nature Immunology 13, 722–728.
Zhou HS, Beevers C, Huang S. 2011. Targets of curcumin. Current Drug Targets 12(3), 332-347.
Godwin Anyim, I. A. Oyemitan, M. A. Aderogba, B. A. Akinpelu (2023), Screening extracts of Asystasia vogeliana Benth. for anti-inflammatory potentials; IJBB, V17, N3, December, P13-25
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