Welcome to International Network for Natural Sciences | INNSpub

Screening Fenugreek genotypes for high callus induction and growth

Research Paper | June 1, 2018

| Download 12

Ghasem Safari, Nasser Mahna, Ali Niazi, Nader Farsad

Key Words:

Int. J. Agron. Agri. Res.12( 6), 146-154, June 2018


IJAAR 2018 [Generate Certificate]


Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients. In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in in vivo samples.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Screening Fenugreek genotypes for high callus induction and growth

Aasim M, Hussain N, Umer EM, Zubair M, Hussain SB, Saeed SH, Rafique TS, Sancak C. 2015. “In vitro shoot regeneration of fenugreek (Trigonella foenum-graecum L.) using different cytokinins.” African Journal of Biotechnology 9(42), 7165-7173.

Afshari E, Ranjbar GA, Kazemitabar SK, Riasat M, Kazemi Poshtmasari H. 2011. Callus induction, somatic embryogenesis and plant regeneration in fenugreek (Trigonella foenum-graecum L.). Iranian Journal of medicinal and aromatic plants 27(51), 147-160.

Bourgaud F, Gravot A, Milesi S, Gontier E. 2001. “Production of plant secondary metabolites: a historical perspective.” Plant Science 1 851-839(5), 61.

Ciura J, Szeliga M, Tyrka M. 2015. “Optimization of in vitro culture conditions for accumulation of diosgenin by fenugreek.” J Med Plants 3(3), 22-25.

Deshpande HA, Bhalsing SR. 2014. “Isolation and characterization of diosgenin from in vitro cultured tissues of Helicteres isora L.” Physiology and molecular biology of plants 20(1), 89-94.

El-Nour MM, Mohammed LS, Saeed BA. 2013. “In vitro Callus induction of Fenugreek (Trigonella foenum-graecum L.) Using Different Media with Different Auxins Concentrations.” Agric. Biol. JN Am 4(3), 243-251.

Elaleem KGA, Magda MS, Badr EA. 2014. “Study of the in vitro callus induction Trigonella foenum-graecum L. from cotyledons and hypocotyls explants supplemented with various plant hormones.” Int. J. Curr. Microbiol. App. Sci 3(12), 486-493.

ElNour ME, Ali AM, Saeed BE. 2015. “Effect of different concentration of Auxin and combination with kinetin on callus initiation on Trigonella foenum-graecum L” International Journal of Technical Research and Applications 3(2), 117-122.

Fazli F, Hardman R. 1971. “Isolation and characterization of steroids and other constituents from Trigonella foenum-graecum.” Phytochemistry 10(10), 2497-2503.

Gomez P, Ortuno A, Del Río JA. 2004. “Ultrastructural changes and diosgenin content in cell suspensions of Trigonella foenum-graecum L. by ethylene treatment.” Plant growth regulation 44(2), 93-99.

Hibi M, Kawashima T, Kodera T, Smirnov SV, Sokolov PM, Sugiyama M, Shimizu S, Yokozeki K, Ogawa J. 2011. “Characterization of Bacillus thuringiensis L-isoleucine dioxygenase for production of useful amino acids.” Applied and environmental microbiology 77(19), 6926-6930.

Huang TK, McDonald KA. 2012. “Bioreactor systems for in vitro production of foreign proteins using plant cell cultures.” Biotechnology advances 30(2), 398-409.

Jamshidi S, Lahouti M, Ganjeali A. 2014. “Assessment of Callus Growth and Bio-production of Diosgenin in.” Bulletin of Environment, Pharmacology and Life Sciences 3.

Khorshidian N, Yousefi Asli MA, Mortazavian M, Mirzaie A, Adeli A. 2016. “Fenugreek: Potential Applications as a Functional Food and Nutraceutical.” Nutrition and Food Sciences Research 16-5, (1)3.

Kodera T, Smirnov SV, Samsonova NN, Kozlov YI, Koyama R, Hibi M, Ogawa J, Yokozeki K, Shimizu S. 2009. “A novel L-isoleucine hydroxylating enzyme, L-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S, 3R, 4S)-4-hydroxyisoleucine.” Biochemical and biophysical research communications 390(3), 506-510.

Mehrafarin A, Qaderi A, Rezazadeh SH, Naghdi Badi H, Noormohammadi GH, Zand E. 2011. “A review on biology, cultivation and biotechnology of fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant and multipurpos 24-6, (37)1.

Nosov AM, Popova EV, Kochkin DV. 2014. Isoprenoid production via plant cell cultures: biosynthesis, accumulation and scaling-up to bioreactors. Production of Biomass and Bioactive Compounds Using Bioreactor Technology, Springer 563-623.

Ogawa J, Kodera T, Smirnov SV, Hibi M, Samsonova NN, Koyama R, Yamanaka H, Mano J, Kawashima T, Yokozeki K, Shimizu S. 2011. “A novel L-isoleucine metabolism in Bacillus thuringiensis generating (2S, 3R, 4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid.” Applied microbiology and biotechnology 89(6), 1929-1938.

Paek K, Chakrabarty D, Hahn EJ. 2005. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Liquid culture systems for in vitro plant propagation, Springer 95-116.

Prabakaran G, Ravimycin T. 2012. “Studies on in vitro propagation and biochemical analysis of Trigonella foenum-graecum L.” Asian Journal of Bio Science 7(1), 88-91.

Provorov NA, Soskov YD, Lutova LA, Sokolova OA, Bairamov SS. 1996. “Investigation of the fenugreek (Trigonella foenum-graecum L.) Genotypes for fresh weight, seed productivity, symbiotic activity, callus formation and aceumulation of steroids.” Euphytica 88(2), 129-138.

Radwan S, Kokate CK. 1980. “Production of higher levels of trigonelline by cell cultures of Trigonella foenum-graecum than by the differentiated plant.” Planta 147(4), 340-344.

Rezaeian S. 2011. Assess of diosgenin production by Trigonella foenum graecum. in vitro condition. American journal of plant physiology 6, 261-268.

Shahabzadeh Z, Heidari B, Faramarzi R. 2013. “Induction of transgenic hairy roots in Trigonella foenum-graceum co-cultivated with Agrobacterium rhizogenes harboring a GFP gene.” Journal of Crop Science and Biotechnology 16(4), 263-268.

Shohael A, Chakrabarty D, Yu K, Hahn EJ, Paek KY. 2005. “Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides.” Journal of biotechnology 120(2), 228-236.

Shi F, Tengfei N, Huimin F. 2015. “4-Hydroxyisoleucine production of recombinant ssp. under optimal corn steep liquor limitation.” Applied microbiology and biotechnology 9(99), 3851-3863.

Smirnov SV, Pavel MK, Tomohiro S, Masakazu H, Makoto S, Sakayu Y, Kenzo O. 2012. “A novel family of bacterial dioxygenases that catalyse the hydroxylation of free L-amino acids.” FEMS microbiology letters 331(2), 97-104.

Zandi P, Saikat Kumar B, Khatibani L, Balogun M, Aremu MO, Sharma M, Ashwin K, Yanshan L. 2015. “Fenugreek (Trigonella foenum-graecum L.) seed: a review of physiological and biochemical properties and their genetic improvement.” Acta Physiologiae Plantarum 37(1), 1714.