Screening for efficient phytase producing bacterial strains from different soils

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Research Paper 01/01/2013
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Screening for efficient phytase producing bacterial strains from different soils

S. Sreedevi, B.N. Reddy
Int. J. Biosci.3( 1), 76-85, January 2013.
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Abstract

Phytic acid (myo-inositol hexakisphosphate) is the main form of phosphate constituting about 80% of the total phosphorus in cereals, legumes and oilseeds used as animal feeds. Phytases are the enzymes which hydrolyze phytic acid to less phosphorylated myo-inositol derivatives releasing inorganic phosphate. The supplementation of animal feed with phytases reduces the cost of diets by removing or reducing the need for supplemental inorganic phosphate and increases the bioavailability of phosphorous in monogastric animals. Rhizosphere soil, Cattle shed soil and poultry farm soil collected from various regions of Hyderabad, Andhra Pradesh, India were used as source material for isolation and screening of phytase producing bacteria. A total of 162 colonies were obtained on wheat extract agar media plates. Fifty four colonies showed clear zones of hydrolysis around them which were replated and hydrolysis efficiency calculated .Twenty one isolates showed more than 50% efficiency which were investigated for phytase production in submerged fermentation. C43 isolate was found to produce significantly high phytase activity than other isolates.

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