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Research Paper | September 1, 2015

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Screening identification of soil bacteria for growth promotion of wheat (Triticum aestivum L.)

Ummay Amara, Yong-Xia Wang, Xiao-Long Cui, Rabia Khalid, Safdar Ali, Ghulam Shabbir, Rifat Hayat

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J. Bio. Env. Sci.7(3), 87-99, September 2015

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Abstract

Plant growth promoting rhizobacteria (PGPR) are well known to impact plant growth by various direct or indirect mechanisms. To investigate the effect of PGPR, a total of nineteen bacterial strains were isolated from wheat rhizospheric soil. These bacterial strains were characterized for their plant growth promoting (PGP) traits like indole-3-acetic acid (IAA) production, phosphate solubilzation, nifH gene amplification, catalase, oxidase, siderophore production, and gram staining. Identification of bacterial strains was done by using 16S rRNA gene sequencing and all bacterial strains were able to produced IAA and solubilize inorganic phosphate. The range for IAA and inorganic phosphate was between 2.81-107.39 μg mL-1 and 14.55 to 556.63 μg mL−1, respectively. Only two bacterial strains were successfully amplified for 400bp nifH gene and most of the isolated bacterial strains were positive for siderophore production test. On the basis of PGP activities nine strains were selected as most promising and tested for wheat growth under controlled chamber experiment. All tested PGPR significantly increased root length, shoot length, dry root weight, and dry shoot weight over control. Three strains i.e. (i) A18, (ii) A28 and (iii) A29 were selected on the basis of their performance under controlled chamber and further testing under pot and field conditions along with application of different doses of NP fertilizer. In pot and field experiments, significant increase was recorded in T6. These results support our hypothesis that use of PGPR or combinations of PGPR and chemical fertilizer can enhance the nutrient use efficiency of fertilizers and crop production.

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Screening identification of soil bacteria for growth promotion of wheat (Triticum aestivum L.)

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