Screening of antibacterial, antifungal and cytotoxic activities of Polygonum hydropiper L. stem extracts

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Research Paper 01/12/2011
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Screening of antibacterial, antifungal and cytotoxic activities of Polygonum hydropiper L. stem extracts

Mohammad Faruk Hasan, Mohammad Motiur Rahman
Int. J. Biosci.1( 6), 47-53, December 2011.
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The aim of the study was to investigate the antibacterial, antifungal and cytotoxic activities of ethanol extract of Polygonum hydropiper stem. Disc diffusion method measuring minimum inhibitory concentration (MIC) was used todemonstrate antibacterial and antifungal activities. Stem extract showed significant antibacterial activities against three gram-positive (Bacillus subtilis, Bacillus megaterium and Staphylococcus aureus) and four gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Shigella sonnei) bacteria resulting 16 to 64 mg/ml MIC values against these bacteria. Further, the antifungal activities of stem extract were not highly remarked but still to be considered as inhibitory to tested fungi. LC50 of the extract against brine shrimp nauplii was 35.46mg/ml and indicates the cytotoxic potentiality of Polygonum hydropiper stem. Results obtained in this study suggest that Polygonum hydropiper stem can also be used as a source of antimicrobial and cytotoxic substances for possible forplant protections.


Akinpelu DA, Onakoyo TM. 2006. Antimicrobial activities of medicinal plants used in folklore remedies in southwestern. African Journal of Biotechnology 5(11), 1078-1081.

Al-Bari MAA, Sayeed MA, Khan A. 2007. In vitro antimicrobial activities and cytotoxicity of ethyl acetate extract from Streptomyces maritimus. Biotechnology 6, 81-85.

Asghari G, Nourallahi H, Havaie SA, Issa L. 2006. Antimicrobial activity of Otostegia persica Boiss. extracts. Research in Pharmaceutical Sciences 1, 53-58.

Chevallier A. 1996. The Encyclopedia of Medicinal Plants. Dorling Kindersley. London, p. 185.

Dash S, Nath LK, Bhise S. 2005. Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. Tropical Journal of Pharmaceutical Research 4, 341-347.

Doughari JH, El-mahmood AM, Tyoyina I. 2008. Antimicrobial activity of leaf extracts of Senna obtusifolia (L.). African Journal of Pharmacy and Pharmacology 2(1), 07-13.

El astal ZY, Aera A, Aam A. 2005. Antimicrobial activity of some medicinal plant extracts in Palestine. Pakistan Journal of Medical Sciences 21(2), 187.

Furukawa T, Eshima A, Kouya M, Takio S, Takano H, One K. 2002. Coordinate expression of genes involved in catchin biosynthesis in Polygonum hydropiper cells. Plant Cell Reports 21, 385-389.

Hossain MS, Hossain MA, Islam R. 2004. Antimicribial and cytotoxic activities of 2-aminobenzoic acid and 2-aminophenol and their coordination complexes with magnesium (Mg-II). Pakistan Journal of Biological Sciences 71, 25-27.

Iwaki K, Koya-Miyata S, Kohno K, Ushio S, Fukuda S. 2006. Antimicrobial activity of Polygonum tinctorium Lour: extract against oral pathogenic bacteria. Nature Medicine 53, 72-79.

Jain SC, Singh R, Jain R. 2008. Antimicrobial and Antioxidant Potentials of Verbesina encelioides (Cav.) Benth. and Hook. Fil ex Gray. Research Journal of Medicinal Plant 2(2), 61-65.

Khan A, Rahman M, Islam S. 2007. Antibacterial, Antifungal and Cytotoxic Activities of Tuberous Roots of Amorphophallus campanulatus. Turkish Journal of Biology 31, 167-172.

Kundu BR, Ara R, Begum MM, Sarkar ZI. 2007. Effect of Bishkatali, Polygonum hydropiper L. plant extracts against the red flour beetle, Tribolium castaneum Herbst. University Journal Zoology Rajshahi University 26, 93-97.

Lin LC, Nalawade SM, Mulabagal V, Yeh MS, Tsay HS. 2003. Micropropagation of Polygonum multiflorum THUNB and Quantitative Analysis of the Anthraquinones, Emodin And Physcion Formed in in vitro Propagated Shoots and Plants. Biological and Pharmaceutical Bulletin 26, 1467-1471.

McLaughlin JL. 1992. Bench-top bioassays for the discovery of bioactive compounds in higher plants. Brenesia p. 220.

Mollah JU, Islam W. 2005. Effect of Polygonum hydropiper L. extracts on the oviposition and egg viability of Callosobruclues cinensis F. (Coleoptera: Bruchidae). Journal of Biological Sciences 12, 101-109.

Nascimento GGF, Lacatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology 31(4), 886-891.

Owolabi  J,  Omogbai  EKI,  Obasuyi  O.  2007. Antifungal and antibacterial activities of ethanolic and aqueous extract of Kigelia african (Bignoniaceae) stem bark. African Journal of Biotechnology 6(14), 882-885.

Sharma R. 2003. Medicinal plants of India-An encyclopedia. Daya publishing house. Delhi-110035. p. 46-47.

Wei W, Xue-Ke Z, Nan W, Yu-jie F, Yuan-gang Z. 2006. Antimicrobial activities of essential oil from Artemisiae argyi leaves. Journal of Forestry Research 17(4), 332-334.