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Screening of cellulolytic fungi and evaluation of biodegradation potentialities of a selected strain on organic solid waste

Kamrun Naher, Md. Showkat Hossain, Nargis Tanjila, Most. Ferdousi Begum

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Int. J. Biosci.7(3), 1-10, September 2015

DOI: http://dx.doi.org/10.12692/ijb/7.3.1-10


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This study focused on the screening of cellulolytic fungi isolated from different sources including soil, humus, agricultural wastes and kitchen wastes. Fungal isolates were isolated from different samples on selective medium and 32 isolates were tested on congo red plate for primary screening. Eight fungal strains have good cellulase potential and showed considerable amount of clear zone. These isolates are identified as Trichoderma virens, T. pseudokoningii, T. harzianum Aspergillus ficuum, A. niger, A. tubingensis, Rhizopus sp. and Fusarium sp. Out of eight isolates T. harzianum had more cellulose hydrolyzing capability and showed better performance on TLC plate. The maximum reducing sugar (365 mg/ml), protein (355 mg/ml) and biomass production (711 mg) was observed in T. harzianum. The maximum CMCase and FPase were also recorded in T. harzianum. This potential cellulolytic strain was further used for biodegradation of OSW with different treatments. The highest volume loss (43.59%) and weight loss (20.31%) was observed in T 1 treatment using kitchen waste and T. harzianum which was greater than 87.01 % and 79.71 %, respectively to control. It indicates T. harzinum is a promising fungus and can be apply in enhancing of organic solid waste biodegradation process to compost.


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Screening of cellulolytic fungi and evaluation of biodegradation potentialities of a selected strain on organic solid waste

Alexopoulos CJ, Mims CW. 1979. Introductory Mycology. 3rd ed. John Wiley & Sons, Inc. New York, USA, 632.

Begum MF. 2006. Screening of Aspergilli from cellulosic waster materials and studies on their cellulolytic properties. PhD thesis, University of Rajshahi, Bangladesh.

Boot C. 1971. The genus Fusarium. Commonwealth Mycological institute. Kew, Surrey, England, 237.

Brierley WB, Jenson ST, Brierley M. 1927. The quantitative study of soil fungi. Proc. 1st Inst. Cong. Soil Science 2, 48-75.

Chandel K, Jandaik S, Kumari V, Sarswati S, Sharma A. 2013. Isolation, purification and screening of cellulolytic fungi from mushroom compost for production of enzyme (cellulase). International Journal of Current Research 5(01), 222-229.

Coman G, Cotarlet M, Bahrim G, Stougaard P. 2008. Increasing the efficiency of screening streptomycetes able to produce glucanases by using insoluble chromogenic substrates. Roum. Biotechnology Letter 13, 20–25.

Ellis MB. 1971. Dematiaceous hypomycetes. Common wealth Mycological Institute. Kew, UK.

Ellis MB. 1976. Demataceous Hyphomycetes, Commonwealth Mycological Institute, Kew, UK.

Fukuda H, Kondo A, Tamalampudi S. 2009. Bioenergy: Sustainable fuels from biomass by yeast and   fungal   whole-cell   biocatalysts. Biochemica Engineering Journal 441, 2-12.

Gautam SP, Bundela PS, Pandey AK, Jamaluddin, Awasthi MK. 2011. Diversity of cellulolytic microbes and the biodegradation of municipal solid waste by a potential strain. International Journal of Microbiology. Article ID 325907, 12pages http://dx.doi.org/10.1155/2012/325907

Gautam SP, Bundela PS, Pandey AK, Jamaluddin, Awasthi MK, Sarsaiya S. 2010. Fusarium sp. from municipal solid waste compost: ability to produce extracellular cellulases enzyme. International Journal of Biotechnology and Bioengineering Research 2(2), 239–245.

Gilman JC. 1957. A Manual of Soil Fungi. 2nd ed. Lowa State. Univ. Press, 450.

Huang GF, Wong JWC, Wu QT, Nagar BB. 2004. Effect of C/N on composting of pig manure with sawdust. Waste Management Research 24(8), 805–813.

Khalid Yang, Kishwar WJ, Rajput NZI, Arijo AG. 2006. Study of cellulolytic soil fungi and two nova species and new medium. Journal of Zhejiang University Science. B 7(6), 459-466.

Khokhar I, Haider MS, Mushtaq S, Mukhtar I. 2012. Isolation and screening of highly cellulolytic filamentous fungi. Scholar Journal of Agricultural Science 2(7), 126-129.

Lowery OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin Phenol reagent. Journal of Biology and Chemistry 193, 265-275.

Lynd LR, Weimer PJ, Van Zyl WH, Pretorious IS. 2002. Microbial cellulose utilization: Fundamentals and Biotechnology, Microbiology. Microbial Molecular Biology Revision 66, 506-577.

Miller GL. 1959. Use of dinitrosalicylic acid for determination of reducing sugar. Analytical Chemistry 31, 426-428.

Muskett AE. 1948. Technique for the examination of seed for the presence of seed borne fungi. Trans British Mycological Society 30, 74-83.

Rahman MR, Anwar MN. 1996. Isolation of thermophilic fungi and screening for their cellulase activity, saccharification, protein and biomass production. Bangladesh Journal of Microbiology 13(182), 57-62.

Rahman A, Begum MF, Rahman M, Bari MA, Ilias GNM, Alam MF. 2011. Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste. Turkish Journal of Biology 35, 183-194.

Ram L, Kaur K, Sharma S. 2014. Screening, isolation and characterization of cellulase producing micro-organisms from soil. International Journal of Pharmaceutical Science Invention 3(1), 12-18.

Sandhu APS, Randhawa GS, Dhugga KS. 2009. Plant  cell  wall  matrix  polysaccharide  biosynthesis. Molecular Plant 2(5), 840–850.

Sharma S, Sumbali G. 2014. Isolation and screening of cellulolytic fungal species associated with lower denomination currency notes, circulating in Jammu city (India). International Journal of Recent Science Research 5(3), 596-600.

Singh H. 2006. Mycoremediation: Fungal Bioremediation. John Wiley & Sons, Hoboken, New Jersey.

Subramanian CV. 1971. Hypomycetes 1st Ed. Indian Council of Agricultural Research, New Delhi.

Sukumaran RK, Singhania RR, Pandey A. 2005. Microbial cellulases-production, applications and challenges. Journal of Sciences and Industrial Researches 64, 832–844.

Ten LN, IM WT, Kim MK, Kang MS, Lee ST. 2004. Development of a plate technique for screening of polysaccharidedegrading microorganisms by using a mixture of insoluble chromogenic substrates. Journal of Microbiological Methods 56, 375–382.

Touchstone JC, Dobbins MF. 1978. Practice of Thin-layer Chromatography.1st. Edn. Willey Interscience. New York.

Zhang YHP, Himmel ME, Mielenz JR. 2006. Outlook for cellulase improvement: Screening and selection strategies. Biotechnology Advances 24, 452–481.


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