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Research Paper | September 1, 2015

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Screening of cellulolytic fungi and evaluation of biodegradation potentialities of a selected strain on organic solid waste

Kamrun Naher, Md. Showkat Hossain, Nargis Tanjila, Most. Ferdousi Begum

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Int. J. Biosci.7(3), 1-10, September 2015

DOI: http://dx.doi.org/10.12692/ijb/7.3.1-10

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Abstract

This study focused on the screening of cellulolytic fungi isolated from different sources including soil, humus, agricultural wastes and kitchen wastes. Fungal isolates were isolated from different samples on selective medium and 32 isolates were tested on congo red plate for primary screening. Eight fungal strains have good cellulase potential and showed considerable amount of clear zone. These isolates are identified as Trichoderma virens, T. pseudokoningii, T. harzianum Aspergillus ficuum, A. niger, A. tubingensis, Rhizopus sp. and Fusarium sp. Out of eight isolates T. harzianum had more cellulose hydrolyzing capability and showed better performance on TLC plate. The maximum reducing sugar (365 mg/ml), protein (355 mg/ml) and biomass production (711 mg) was observed in T. harzianum. The maximum CMCase and FPase were also recorded in T. harzianum. This potential cellulolytic strain was further used for biodegradation of OSW with different treatments. The highest volume loss (43.59%) and weight loss (20.31%) was observed in T 1 treatment using kitchen waste and T. harzianum which was greater than 87.01 % and 79.71 %, respectively to control. It indicates T. harzinum is a promising fungus and can be apply in enhancing of organic solid waste biodegradation process to compost.

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