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Screening of effective antagonists from potato rhizosphere against bacterial wilt pathogen

M. Ibrahim Tahir, M. Inam-ul-Haq, M. Ashfaq, Nadeem Akhtar Abbasi, Haris Butt, Hira Ghazal

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Int. J. Biosci.8(2), 228-240, February 2016

DOI: http://dx.doi.org/10.12692/ijb/8.2.228-240

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Abstract

Potato is the most consumed vegetable while stands 4thamong food crops after wheat, rice, and maize in terms of production. Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases throughout the world causing substantial losses in potato and in other important crops. This study was embarked to search for biocontrol of pathogen, keeping in view recent reports of disease incidence in major potato growing areas of Pakistan. In this study, several potato growing areas of unjab (Pakistan) were visited to study the bacterial community residing in potato rhizosphere and also screen bacterial antagonists against the virulent strains (Rs9, Rs17 and R43) of R. solanacearum. The results revealed that out of 221 rhizobacterial isolates, Bacillus spp. (101) were dominantly isolated from potato rhizosphere, followed by Pseudomonas spp. (76) and Serratia spp. (44). All these isolates were tested for antagonism using dual culture plate method which showed 11 isolates to be highly antagonistic against either isolate of R. solanacearum. Against Rs9, Rs17, Rs43 and GMI1000 highest zone of inhibition was recorded with B28 (12.3 mm), P11 (10.2 mm), B9 (9.9 mm) and B85 (11.7 mm) respectively. Culture filtrate (CF) of antagonists was also checked against live R. solanacearum cells which also showed inhibition in liquid medium. Antagonistic isolates were tested for plant growth promoting (PGP) traits i.e. indole acetic acid (IAA), siderophore production, P-solubilization, root colonization, and chitinase production. The isolates B28 and B85 were potential isolates possessing antagonistic activity along with several PGP traits.

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Screening of effective antagonists from potato rhizosphere against bacterial wilt pathogen

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