Welcome to International Network for Natural Sciences | INNSpub

Screening of PHA (poly hydroxyalkanoate) producing bacteria from diverse sources

Research Paper | December 1, 2011

| Download 3

Nandini Phanse, Amruta Chincholikar, Bhavesh Patel, Pragya Rathore, Priti Vyas1, Mital Patel

Key Words:

Int. J. Biosci.1( 6), 27-32, December 2011


IJB 2011 [Generate Certificate]


Synthetic plastics are non-degradable and cause waste disposal problems leading to environmental pollution. Bioplastics (polyhydroxyalkanoates) are considered good substitutes for petroleum derived synthetic plastics because of their similar physical and chemical properties. Main advantage of bioplastics is that they are of biological origin and can get degraded completely to CO2 and water under natural environment by the enzymatic activities of microorganisms. Poly-β-hydroxyalkanoates (PHA) are polyesters of various hydroxyalkanoates, synthesized by numerous bacteria as an intracellular carbon and energy storage compound under limited nutrient conditions and with excess carbon. Poly- β- hydroxy butyrate (PHB) is the best known polyhydroxyalkanoate. Considering the industrial interest of PHA, this work has been undertaken for the screening of PHA producing bacteria from diverse sources. In the present study, an attempt was made to isolate efficient PHB producing bacteria from diverse environmental samples. Different industrial wastes and soil samples were screened for bacteria possessing the ability to accumulate poly hydroxyalkanoate (PHA) granules. About 23 bacterial isolates were found to be promising PHA accumulating bacteria. Screening for PHA producers was performed by using E 2 medium. Accumulation of PHB granules in the organisms was analyzed by Sudan black method.


Copyright © 2011
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Screening of PHA (poly hydroxyalkanoate) producing bacteria from diverse sources

Arun A, Murrugappan RM, David Ravindran, Veeramanikandan V, Balaji S. 2006. Utilization of various industrial wastes for the production of poly-hydroxy butyrate (PHB) by Alcaligenes eutrophus. African Journal of Biotechnology 5, 1524-1527.

Ayub ND, Pettinari MJ, Ruiz JA, Lopez NJ. 2004. A polyhydrobutyrate –producing Pseudomonas sp. isolated from Antarctic environments with high stress resistance. Current Microbiology, 49, 170-174.

Burdon KL, Stokes JC, Kimbrough CE. 1942a. Studies of the common aerobic spore-forming Bacilli staining for fat with Sudan Black B- stain. Journal of Bacteriology, 43, 717-724.

Full TD, DO Jung, MT Madigan. 2006. Production of poly-β-hydroxyalkanoates from soy molasses oligosaccharides by new, rapidly growing Bacillus species. Letters of Applied Microbiology 43, 377-384.

Hartman TL. 1940. The use of Sudan Black B as a bacterial fat stain. Staining Technology, 15, 23-28.

Holt JG, Krig NR, Sneath Pefer HA, Staley JT, Williams ST. 1994. Bergey’s Manual of Determinative Bacteriology 9th edition, Williams and Wilkins, Baltimore.

Juan ML, Gonzalez LW, Walker GC. 1998. A Novel Screening Method for Isolating Exopolysaccharide deficient Mutants. Applied and Environmental Microbiology 64, 4600-4602.

Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI. 1994. Production of Poly (3hydroxybutyric– co-hydroxyl valeric acid) by fed batch culture of Alcaligens eutrophus with substrate control using on line glucose analyzer. Enzyme Microbial Technology 16, 556-561.

Kitamara S, Doi Y. 2004. Staining method of poly (3- alkanoic acid) producing bacteria by Nile blue. Biotechnological Techniques 8, 345-350.

Lafferty RM, Korsatko B, Korsatko W. 1988. Microbial  production  of  poly-  -hydroxybutyric  acid. Biotechnology Special microbial Processes, 136-176.

Lageveen RG, GW Huisman, H Preusting, P Ketelaar, G Eggink, B Witholt. 1988. Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on formation and composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates. Applied Environmental Microbiology 54, 2924-2932.

Lee  SY.  1996. Bacterial Polyhydroxyalkanoates. Biotechnology and Bioengineering, 49:1-14. Microbiology Review, 103, 131-140.

Madison LL, Huisman GW. 1999. Metabolic engineering of poly(3 hydroxyalkanoates): from DNA to plastic. Microbiology Molecular Biology Reviews. 63, 21–53.

Otari SV, and Ghosh JS. 2009. Production and Characterization of the Polymer Polyhydroxy Butyrate-copolyhydroxy Valerate by Bacillus Megaterium NCIM 2475. Current Research Journal of Biological Sciences 1(2), 23-26.

Page WJ. 1992. Production of Polyhydroxyalkanoates by Azotobacter vinelandii Strain UWD in Beet Molasses Culture. FEMS Microbiological Reviews, 103, 149-158.

Parshad J, Suneja S, Kukeja K, Lakshminarayana K. 2001. Poly hydroxybutyrate production by Azotobacter chroococcum. Foliar Microbiology, 46 (4), 315-320.

Roy B, Banerjee R, Chatterjee S. 2009. Isolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester. Indian Journal of Experimental Biology, 47: 250-256.

Santhan, A. and Sasidharan. S. 2010. Microbial production of polyhydroxy alkanotes (PHA) from Alcaligens spp. and Pseudomonas oleovorans using different carbon sources. African Journal of Biotechnology, 9 (21), 3144-3150.

Sujatha K, Mahalakshmi A, Snehbagarathai. 2005. A study on accumulation of PHB in native Pseudomonas isolates LDC-5 and LDC-25. Indian Journal of Biotechnology, 4, 216-221.

Yilmaz M, Soran HB, Yavuz. 2005. Determination of poly-β-hydroxybutyrate (PHB) production by some Bacillus spp. World Journal of Microbiology and Biotechnology, 21 (4), 565-566.

Yuksedag ZN, Aslim B, Beyadliti X, Mercan N. 2004. Effect of carbon and nitrogen sources and incubation time on poly-β-hydroxybutyrate (PHB) synthesis by Bacillus subtilis 25 and Bacillus megaterium 12. African Journal of Biotechnology, 3 (1): 63-66.