Screening plant secondary metabolites from callus cultures of sunflower

Paper Details

Research Paper 01/10/2018
Views (1044)
current_issue_feature_image
publication_file

Screening plant secondary metabolites from callus cultures of sunflower

Noor-e-Saba Khaskheli, Komal Siddiqui, Mehtab Hussain Nizamani, Hafiza Faiza
Int. J. Biosci. 13(4), 350-356, October 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Plants produce a diverse group of secondary metabolites to guard themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation. In present investigation callus cultures were used to monitor the effect of mutagenic compounds on the plant secondary metabolites. Sunflower plant was mutagenized using different concentrations of mutagen namely EMS. Callus cultures were maintained using various combinations of phytohormones. Callus were obtained using phytohormones NAA, Thiamin HCL, 2,4-D. highest reducing sugars were obtained in 0.2% EMS, and highest reducing power was observed in the callus culture of 0.1% EMS.

Ao C. 2008. “Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract,” Food control vol. 19, pp. 940-948.

Berrios E. 1999. “Genetic control of in vitroorganogenesis in recombinant inbred lines of sunflower (Helianthus annuus L.),” Plant breeding vol. 118, pp. 359-361.

Berrios EF. 1999. “Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower,” Plant Cell, Tissue and Organ Culture vol. 59, pp. 65-69.

Chraibi KM. 1992. “A genotype-independent system of regeneration from cotyledons of sunflower (Helianthus annuus L.) the role of ethylene,” Plant Science vol. 86, pp. 215-221.

Classics Lowry O. 1951. “Protein measurement with the Folin phenol reagent,” J biol Chem vol. 193, pp. 265-75.

Dhaka N, Kothari S. 2002. “Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Helianthus annuus L,” Plant cell reports vol. 21, pp. 29-34.

Dotson SB. 1986. “Sodium azide as a maize tissue culture mutagen,” University of Minnesota.

Espinasse A, Lay C. 1989. “Shoot regeneration of callus derived from globular to torpedo embryos from 59 sunflower genotypes,” Crop Science vol. 29, pp. 201-205.

Franco D. 2008. “Polyphenols from plant materials: extraction and antioxidant power,” Electron. J. Environ. Agric. Food Chem vol. 7, pp. 3210-3216.

Gleason DF. 1974. “Prediction of prognosis for prostatic adenocarcinoma by combined histological grading and clinical staging,” The Journal of urology vol. 111, pp. 58-64, 1974.

IATA, “technical Report Series,” Manual on mutation breeding, p. International Atomic Energy agency. Vienna, 1977.

Kane NC, Rieseberg LH. 2007. Selective sweeps reveal candidate genes for adaptation to drought and salt tolerance in common sunflower, Helianthus annuus. Genetics 2007 Jan 21.

Kleinhofs A. 1978. “Induction and selection of specific gene mutations in Hordeum and Pisum,” Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis vol. 51, pp. 29-35.

Lide DR. 2000. “Handbook of Chemistry and Physics 81st edition, Sec. 11,” ed: CRC Press, Boca Raton.

Matkowski A. 2008. “Plant in vitro culture for the production of antioxidants—a review,” Biotechnology advances vol. 26, pp. 548-560.

Miller GL. 1959. “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Anal chem, vol. 31, pp. 426-428.

Montogomery R. 1961. “Further studies of the phenol-sulphuric acid reagent for carbohydrate,” Biochem. Bioph. Acta vol. 48, pp. 591-593.

Mulabagal V, Tsay HS. 2004. “Plant cell cultures-an alternative and efficient source for the production of biologically important secondary metabolites,” Int J Appl Sci Eng vol. 2, pp. 29-48.

Murashige T, Skoog F. 1962. “A revised medium for rapid growth and bio assays with tobacco tissue cultures,” Physiologia plantarum vol. 15, pp. 473-497.

Owais W. 1983. “A mutagenic metabolite synthesized by Salmonella typhimurium grown in the presence of azide is azidoalanine,” Mutation Research/Genetic Toxicology vol. 118, pp. 229-239.

Oyaizu M. 1986. “Studies on products of browning reaction,” The Japanese Journal of Nutrition and Dietetics vol. 44, pp. 307-315.

Power CJ. 1987. “Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos,” American journal of botany pp. 497-503.

Prieto P. 1999. “Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E,” Analytical biochemistry vol. 269, pp. 337-341.

Singleton VL, Rossi JA. 1965. “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents,” American journal of Enology and Viticulture, vol. 16, pp. 144-158.

Tadhani M. 2007. “In vitro antioxidant activities of Stevia rebaudiana leaves and callus,” Journal of Food Composition and Analysis vol. 20, pp. 323-329.

Verpoorte R. 2002. “Biotechnology for the production of plant secondary metabolites,” Phytochemistry reviews vol. 1, pp. 13-25.

Wang A. 1987. “Mutagenesis of tissue cultures,” Maize Genet. Newsl vol. 61, pp. 81-83.

Weinhold PA, Rethy VB. 1974. “Separation, purification, and characterization of ethanolamine kinase and choline kinase from rat liver,” Biochemistry vol. 13, pp. 5135-5141.

Witrzens B. 1988. “Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H. tuberosus x H. annuus),” Plant Cell, Tissue and Organ Culture vol. 13, pp. 61-76, 1988.

Related Articles

Optimizing soybean (Glycine max L. Merr.) performance through rhizobial inoculation and planting density in Kétou, Benin

Mahougnon Charlotte Carmelle Zoundji*, Ibouraïman Balogoun, Pascal Gbenou, Tobi Moriaque Akplo, Carlosse Djeho, Félix Kouélo Alladassi, Int. J. Biosci. 28(6), 99-107, June 2026.

Genetic admixture and the philosophy of diplomacy in central Asia: Evidence from intercultural dialogue, governance and genomic data

Shafee Ur Rehman, Waqar Ahmed Khan, Iqra Jamil, Muhammad Abdullah, Int. J. Biosci. 28(6), 89-98, June 2026.

Synthesizing and integrating environmental awareness and bio-intensive gardening under the Gulayan sa Paaralan (SIBUG) extension project

Violeta F. Collado*, Analyn V. Sagun, Angelina T. Gonzales, Marilyn D. Respicio, Int. J. Biosci. 28(6), 82-88, June 2026.

Diversity of insects related to maize (Zea mays) production in Ferkéssédougou region, Côte d’Ivoire

Fondio Drissa, Dao Hassane, Soro Lacina*, Sib Ollo, Kouadio Roger Hosphade Kouassi, Soro Senan, Yeboue N’guessan Lucie, Int. J. Biosci. 28(6), 75-81, June 2026.

Diuretic activity assessment of an aqueous extract of Zanthoxylum gilletii (Rutaceae) stem bark in rats

Akoua Jeanne Kanga*, Essoi Kouametchi Hermann, Françoise Assamala Fossou, Kacou Jules Marius Djetouan, Kouao Augustin Amonkan, Int. J. Biosci. 28(6), 68-74, June 2026.

Phytochemical investigation and in vitro evaluation of cholinesterase inhibitory and antioxidant properties of Aglaonema hookerianum stems

K. M. Monirul Islam, Simin Shabnam Lopa, Joya Rani, Md. Aslam Sheikh, Md. Golam Sadik*, Int. J. Biosci. 28(6), 60-67, June 2026.

Comparative responses of rice (Oryza sativa L.) to iron toxicity, drought and salinity stress: Morphological, physiological, biochemical and molecular regulation mechanisms

Yaya Touré*, Brahima André Soumahoro, Arthur Martin Affery, Tchoa Koné, Mongomaké Koné, Int. J. Biosci. 28(6), 37-50, June 2026.