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Screening plant secondary metabolites from callus cultures of sunflower

Research Paper | October 1, 2018

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Noor-e-Saba Khaskheli, Komal Siddiqui, Mehtab Hussain Nizamani, Hafiza Faiza

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Int. J. Biosci.13( 4), 350-356, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.350-356


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Plants produce a diverse group of secondary metabolites to guard themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation. In present investigation callus cultures were used to monitor the effect of mutagenic compounds on the plant secondary metabolites. Sunflower plant was mutagenized using different concentrations of mutagen namely EMS. Callus cultures were maintained using various combinations of phytohormones. Callus were obtained using phytohormones NAA, Thiamin HCL, 2,4-D. highest reducing sugars were obtained in 0.2% EMS, and highest reducing power was observed in the callus culture of 0.1% EMS.


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