Search for polymorphism in growth and differentiation factor 9 (GDF9) gene in prolific beetal and tali goats (Capra hircus)

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Research Paper 01/04/2014
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Search for polymorphism in growth and differentiation factor 9 (GDF9) gene in prolific beetal and tali goats (Capra hircus)

Morteza Hadizadeh, Mohammad Reza Mohammadbadi, Ali Niazi, Ali Esmailizadeh, Yasser Mahdizadeh Gazooei
J. Bio. Env. Sci.4( 4), 186-191, April 2014.
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Growth and differentiation factor 9 gene is one of the main genes controlling prolificacy in sheep and goat. Mutations in this gene affect litter size in a dosage sensitive manner so that heterozygous animals have litter size records higher than wild types while the mutant homozygous animals are sterile. In this study, we analyzed exon 2 of the growth and differentiation factor 9 as a candidate gene for high prolificacy in Tali and Beetal goats, the common prolific breeds of goats reared in southern and southeast Iran. The results showed a cytosine to thymine nucleotide change at the position of 881 in Beetal goats causing the replacement of alanine with valine at the position of 273 in the unprocessed protein. The results provided further evidence that this amino acid change can affect functions of the protein through changing the conformation of its third structure in Beetal goats.


Sadighi M, Bodensteiner KJ, Beattie AE, Galloway SM. 2002. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5. Animal Genetics 33:244-245.

Nicol L, Bishop SC, Pong-Wong R, Bendixen C, Holm L, Rhind SM, McNeilly AS. 2009. Homozygosity for a single base-pair mutation in the oocytespecific GDF9 gene results in sterility in Thoka sheep. Reproduction 138:921-933. 10.1530/REP-09-0193

Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R, Galloway SM. 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of Reproduction 70: 900-909.

Ran XQ, Lin JB, Du ZY, Qing C, Wang JF. 2009. Diversity of BMP15 and GDF9 genes in White Goat of Guizhou province and evolution of the encoded proteins. Zool. Res 30: 593-602. 10.3724/SP.J.1141.2009.06593

Bodensteiner KJ, Clay CM, Moeller CL, Sawyer HR. 1999. Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. Biology of Reproduction 60: 381-386. 10.1095/biolreprod60.2.381

Juengel JL, Hudson NL, Heath DA, Smith P, Reader KL, Lawrence SB, O’Connell AR, Laitinen MPE, Cranfield M, Groome NP, Ritvos O, McNatty KP. 2002. Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep. Biology of Reproduction 67: 1777-1789. 10.1095

Juengel JL, Bodensteiner KJ, Heath DA, Hudson NL, Moeller CL, Smith P, Galloway SM, Davis GH, Sawyer HR, McNatty KP. 2004. Physiology of GDF9 and BMP15 signalling molecules. Animal Reproduction Science 82: 447-460. 10.1016/j.anireprosci

Feng T, Geng CX, Lang XZ, Chu MX, Cao GL, Di R, Fang L, Chen HQ, Liu XL, Li N. 2011. Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats. Molecular Biology Reports 38: 5189-5197. 10.1007/s11259-011-9476-8

McNatty KP, Galloway SM, Wilson T, Smith P, Hudson NL, O’Connell A, Bibby AH, Heath DA, Davis GH, Hanrahan JP, Juengel JL. 2005. Physiological effects of major genes affecting ovulation rate in sheep. Genetic Selection Evolution 37: S25–S38. 10.1051/gse:200402

Vitt UA, McGee EA, Hayashi M, Hsueh AJ. 2004. In vivo treatment with GDF9 stimulates primordial and primary follicle progression and theca cell marker CYP17 in ovaries of immature rats. Endocrinology 141: 3814-3820.

Miller SA, Dykes DD, Polesky HF. 1998. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16(3): 1215.

Wu ZH, Chu X, Li XW, Fang L, Ye SC, Liu ZH, Chen GH. 2006. PCR-SSCP analysis on exon 2 of growth differentiation factor 9 gene in goats. Scientia Agricultura Sinica 39: 802-808.

Galloway SM,  McNatty KP,  Cambridge  LM, Laitinen MP, Jennifer JL, Jokiranta S, McLaren RJ, Luiro K, Dodds KG, Montgomery GW, Beattie AE, Davis GH,  Ritvos O.  2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics 25: 279-283.

Ren ZZ, Cai Hf, Luo WX, Liu RY. 2010. GDF9 and BMP15 Genes Analysis of Genetic Variation on Qianbei-pockmarked Goats. China Animal Husbandry & Veterinary Medicine 37(7): 99-102.

Silva BD, Castro EA, Souza CJ, Paiva SR, Sartori R, Franco MM, Azevedo HC, Silva TA, Vieira AM, Neves JP, Melo EO. 2011. A new polymorphism in the Growth and Differentiation Factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Animal Genetics 42(1): 89-92. 10.1111/j.1365-2052.2010.02078.x

Wang Y, Yuanxiao L, Nana Z,  Zhanbin W, Junyan B. 2011.  Polymorphism of Exon 2 of BMP15 Gene and Its Relationship with Litter Size of Two Chinese Goats. Asian-Australian Journal of Animal Science 24(7): 905-911.