Seasonal variation of acid phosphatase and dehydrogenase activity in natural and artificial habitats of hazel

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Research Paper 01/11/2014
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Seasonal variation of acid phosphatase and dehydrogenase activity in natural and artificial habitats of hazel

Farhang Moraghebi
Int. J. Agron. Agri. Res.5( 5), 216-222, November 2014.
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The hazels (Corylus avellana) are seen in north region of Iran and many stands grow in upper area of northern slop of Alborz Mountains. Soil is an effective living factor in ecosystem balance. There are many biological and biochemical process in soil which rapidly reacts to environmental stresses. Enzymes have essential roles in these processes. The aim of this research was to study the activities of acid phosphatase and dehydrogenase in two natural hazel habitats (Makesh and Fandoghlo) and compare to their activities in an artificial habitat (Alborz). Soil sampling was done in spring and summer. The activity of acid phosphatase and dehydrogenase was evaluated by enzyme- substrate reaction. Enzymes had more activity in summer in all studied habitats. The more activity of acid phosphatase can be related to growth of hazel roots and secretion of enzyme during growth season. Enzymes showed more activity in Fandoghlo habitat in compared to Makesh ones. The amount of nitrogen, phosphorus and organic matter was less in Fandoghlo habitat which causes more activity of microorganisms to supply plants needs and compensation of low nutrients in soil.


AdamsMA. 1992. Phosphatase activity and phosphorus fractions in Karri (Eucalyptus diversicolor F. Muell.) forest soils. Biology and Fertility of Soils 14, 200-204.

Ajwa HA, Dell CJ, Rice CW. 1999. Changes in enzyme activities and microbial biomass of tallgrass prairie soil as related to burning and nitrogen fertilization. Soil Biology and Biochemistry 31, 769– 777.

Bremmer JM, Mulvaney CS. 1982. Nitrogen total. In: ‘Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties’. (Eds AL Page, RH Mill Keenpp. 595–624. (ASA: Madison).

Caldwell BA, Griffiths RP, Sollins P. 1999. Soil enzyme response to vegetation disturbance in two lowland Costa Rican soils. Soil Biology and Biochemistry 31, 1603–1608.

Clarholm M. 1993. Microbial biomass P, labile P and acid phosphatase activity in the humus layer of a spruce forest, after repeated additions of fertilizers. Biology and Fertility of Soils 16, 287-292.

Dick RP. 1994. Soil enzyme activities as indicators of soil quality. In: Doran, J. W., Coleman, D. C., Bezdicek, D. F., Stewart, B. A., (Eds.) Defining Soil Quality for a Sustainable Environment. Soil Sci. Soc. Am. Madison WI, 108-123 P.

Dick RP, Rasmussen PE, Herle EA. 1988. Influence of long term residue management on soil enzyme activities in relation to soil chemical properties of a wheat-fallow system. Biological Fertilizers and Soils 6, 158-164.

Gil-Sotres F, Trasar-Cepeda C, Leiros MC, Seoane S. 2005. Different approaches to evaluate soil quality using biochemical properties. Soil Biology and Biochemistry 37, 877–887.

Grierson PF, Adams MA. 2000. Plant species affect acid phosphatase, ergosterol and microbial P in a Jarrah (Eucalyptus marginataDonnex Sm.) forest in south-western Australia. Soil Biology and Biochemistry 32, 1817–1827.

Kandeler E. 2007. Physiological and biochemical methods for studying soil biota and their function. In: Paul E.ASoil Microbiology, Ecology and Biochemistry. Acad. Press, Oxford, UK. 53-80.

Klein DA, Sorensen DL, Redente EF. 1985. Soil enzymes: A predictor of reclamation potential and progress. In: Tate, R.L., Klein, D.A. (Eds.), Soil Reclamation Processes. Microbiol. Analysis. application. Marcel Dekker, New York, . 273–340 P.

Kramer S, Green DM. 2000. Acid and alkaline phosphatase dynamics and their relationship to soil microclimate in semiarid woodland. Soil Biology and Biochemistry 32, 179-188.

Matinizadeh M, Korori SAA, Teimouri M, Praznik W. 2008. Enzyme Activities in Undisturbed and Disturbed Forest Soils Under Oak (Quercusbrantii var. persica) as Affected by Soil Depth and Seasonal Variation. Asian Journal of Plant science 7(4), 368-374.

Moraghebi F. 2001. Study and introduction of Corylus maxima by use of morphological characters and enzymatic. Pajouhesh and Sazandegi 52, 45-51.

Moraghebi F, Matinizadeh M, Khanjani-Shirazi B. 2010. Mycorrhizal symbiosis in Corylus avellanaL.and acid phosphatase activity in Makesh and Fandoghlo habitats. Plant and Ecosystem 24, 13-23.

Moraghebi F, Matinizadeh M, Khanjani-Shirazi B, Teimouri M, Afdideh F. 2012. Seasonal variation of urease and alkaline phosphatase activity in natural and artificial habitats of hazel.Journal  of  Medicinal  Plants  Research  6(14), 2714-2720,

Ohlinger R. 1996. Acid and alkaline phosphomonoesterase activity with the substrate p-nitrophenyl phosphate. In: (Eds) Methods in soil biology. Springer-Verlag Berlin. 210-214.

Olsen SR, Cole LV, Watanabe  FS, Deanm LA. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Circ. United States Dep. Agric. no. 939, Washington DC.

Sedia EG, Ehrenfeld JG. 2006. Differential effects of lichens and mosses on soil enzyme activity and litter decomposition. Biology and Fertility of Soils 43, 177–189.

Shirvany A. 2004. Investigation  of   healthy  and unhealthy Ulmus  glabra  Hudson  to  find  resistant genotypes against in   north of Iran. PhD thesis, natural resources faculty, Tehran university, Iran. 550 p.

Sinsabaugh RL. Carreiro MM, Alvarez S. 2002. Enzyme and microbial dynamics of litter Decomposition. In: Burns R. G., Dick W. A. (Eds) Enzymes in the environment. Marcel Dekker, New York, 249-266 P.

Staddon WJ, Duchesne LC, Trevors JT. 1998.. Acid phosphatase, alkaline phosphatase and arylsulfatase activities in soils from a jack pine (Pinusbanksiana Lamb.) ecosystem after clear-cutting, prescribed burning, and scarification. Biology and Fertility of Soils 27, 1-4.

Tabatabai MA. 1994. Soil enzymes. In: Page, A. L. Miller, R. H. Keeney, D. R. (Eds.) Methods of Soil Analysis Part 2, Agronomy 9, American Society of Agronomy Madison Wis. 903-947 P.

Tabatabai MA, Dick WA. 2002. Enzymes in soil. In: Burns, R. G., Dick, W. A. (Eds) Enzymes in the environment. Marcel Dekker, New York, 567–596.

Turco RF, Kennedy AC, Jawson MD. 1994. Microbial indicators of soil quality. In: Doran JW, Coleman DC, Bezdicek, D. F, Stewart, B. A. (Eds) Defining soil quality for a sustainable environment. Soil Science Society of America Journal., Special Publication. 35, 73-90.

Walkley A, Black IA. 1934. An examination of degtjareffmethod for determining soil organic matter and a proposed modification of the chromi acid titration method. Soil Science 37, 29-37.

Waldrop MP, Balser TC, Firestone MK. 2000. Linking microbial community composition to function in a tropical soil. Soil Biology and Biochemistry 32, 1837–1848.