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Secondary metabolite composition & Anti-inflammatory potential of Pansit-pansitan (Peperonia pelludica Linn): An indigenous vegetable

Research Paper | August 1, 2020

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Danielle Erika Y Apatas, Joji B Cauan, Abigail M Malazo, Carlo T Raganit, Jay Andrea Vea D Israel, Ramelo B Ramirez Jr

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Int. J. Biosci.17( 2), 278-284, August 2020

DOI: http://dx.doi.org/10.12692/ijb/17.2.278-284


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The research was conducted to determine if Pansit- pansitan (Peperomia pellucida Linn) aqueous leaf extract have in – vitro anti-inflammatory potentials. Fresh leaves collected were air dried and subjected to aqueous extraction and prepared in different doses (200, 400, 600, 800 and 1000ug/mL). Inhibitions of HRBC lysis and protein denaturation were used to evaluate in-vitro anti-inflammatory activity of the extract. Based on the findings of the study, Pansit- pansitan (Peperomia pellucida Linn) shows that with increasing dosage, membrane stabilization and protein protective property also increases. The anti-inflammatory effect of the aqueous extract on the plant can be attributed to the presence of its secondary metabolites which is known to have anti-inflammatory property only that these metabolites are not sufficient enough when used in low dosage.


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Secondary metabolite composition & Anti-inflammatory potential of Pansit-pansitan (Peperonia pelludica Linn): An indigenous vegetable

Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee M. 2018. The crucial roles of inflammatory mediators in inflammation: A Review. Veterinary World, 11(5), 627-635.

Alinejhad D, Asayesh MA, Asayesh M. 2016. Determination of the Anti-inflammatory property of Tannins from the Rind of Calamansi (Citrus microcarpa, Rutaceae) Journal of International Oral Health 8(5), 546-553.

Alves NS, Setzer W, da Silva JK. 2019. The chemistry and biological activities of Peperomia pellucida (Piperaceae): A critical review, Journal of Ethnopharmacology 232, 90-102,

Anosike, Chioma & Obidoa, Onyechi & Ezeanyika, Lawrence. 2012. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru: journal of Faculty of Pharmacy, Tehran University of Medical Sciences 20, 76.

Ansar W, Ghosh S. 2016. Inflammation and Inflammatory Diseases, Markers, and Mediators: Role of CRP in Some Inflammatory Diseases. Biology of C Reactive Protein in Health and Disease 67-107. https://doi.org/10.1007/978-81-322-2680-2_4

Banerjee S, Chanda A, Adhikari A, Das A, Biswas S. 2014. Evaluation of Phytochemical Screening and Anti Inflammatory Activity of Leaves and Stem of Mikania scandens (L.) Wild. Annals of Medical and Health Sciences Research 4(4),

Barbosa Filho, Jose & Piuvezam, Marcia & Moura, Marcelo & Silva, Marcelo & Lima, Karla & da-Cunha, Emídio & Fechine, Ivana & Takemura, Orlando. 2006. Anti-inflammatory activity of alkaloids: A twenty-century review. Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy- Rev. Bras. Farmacogn. 16.

Brenner DR, Scherer D, Muir K, Schildkraut J, Boffetta P, Spitz MR, Le Marchand L, Chan AT, Goode EL, Ulrich CM, Hung RJ. 2014. A review of the application of inflammatory biomarkers in epidemiologic cancer research. Cancer epidemiology, biomarkers & prevention: A publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 23(9),

Böttger A, Vothknecht U, Bolle C, Wolf A. 2018. Plant Secondary Metabolites and Their General Function in Plants. In: Lessons on Caffeine, Cannabis & Co. Learning Materials in Biosciences. Springer, Cham

Chandra S, Dey P, Bhattacharya S. 2012. Preliminary in vitro assessment of anti-inflammatory property of Mikania scandens flower extract. J Adv Pharm Edu Res 2(1), 25-31.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2(1), S178-80.

Chatterjee P, Chandra S, Dey P, Bhattacharya S. 2012. Evaluation of anti-inflammatory effects of green tea and black tea: A comparative in vitro study. Journal of Advanced Pharmaceutical Technology & Research 3(2), 136-138.

Choi J, Jung HJ, Lee KT, Park HJ. 2005. Antinociceptive and anti-inflammatory effects of the saponin and sapogenins obtained from the stem of Akebia quinata. J. Med. Food. 8(1), 78‐85.

Chowdhury A, Azam S, Jainul MA, Faruq KO, Islam A. 2014. Antibacterial activities and in vitro anti-inflammatory (Membrane stability) proterites of methanolic extracts of Gardenia coronaria leaves. International Journal of Microbiology.

Eckersall PD. 2008. Proteins Proteomics and the Dysproteinemias. In: Kaneko JJ, Harvey JW, Brussm L, Eds, Clinical Biochemistry of Domestic Animals, 6th Edition, Academic Press, San Diego 117-155.

Florence NT, Huguette STS, Hubert DJ. 2017. Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats. BMC Complement Altern Med 17, 188.

Hassan HS, Sule MI, Musa AM, Musa KY, Abubakar MS, Hassan AS. 2011. Anti-inflammatory activity of crude saponin extracts from five Nigerian medicinal plants. African journal of traditional, complementary, and alternative medicines: AJTCAM 9(2), 250-255.

Hossain H, Al-Mansur M, Akter S, Sara UA, Dr Md, Jahangir AA. 2014. Evaluation of anti-inflammatory activity and total tannin content from the leaves of Bacopa monnieri (Linn.). IJPSR 5. 1246-1252.

Jainul MA, Chowdhury A, Mamun AR, Shadman, Azam, Shofiul, Shams K. 2013. Human red blood cell membrane stability testing for the estimation of anti-inflammatory activity of methanolic extract of Millettia pachycarpa benth leaves. International Journal of Pharmaceutical Sciences and Research 4, 4587-4590.

Jang K, Kim HK, Han MH, Oh YN, Yoon H, Chung YH. Choi YH. 2013. Anti-inflammatory effects of saponins derived from the roots of Platycodon grandiflorus in lipopolysa ccharide‑stimulated BV2 microglial cells. International Journal of Molecular Medicine 31, 1357-1366.

Solomkin JS, Simmons RL. 1983 Cellular and Subcellular Mediators of Acute Inflammation, Surgical Clinics of North America, Volume 63, Issue 2, 1983, Pages 225-243,

Kardile MM, Umesh, Shaikh HG, Sameer, Patil C. 2016. Membrane Stabilization Assay for Anti-Inflammatory Activity Yields False Positive Results For Samples Containing Traces of Ethanol and Methanol. World Journal of Pharmacy and Pharmaceutical Sciences 5, 493-497.

King TC. 2007. 2-Inflammation, Inflammatory Mediators, and Immune-Mediated Disease, Elsevier’s Integrated Pathology, Mosby, 2007,

Kumari CS, Yasmin N, Hussain MR, Babuselvam M. 2015. Invitro anti-inflammatory and anti-arthritic property of Rhizopora mucronata leaves. Intern J Pharma Scie Res 6, 482-485.

Leelaprakash G, Dass SM. 2011. Invitro anti-inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development and Research 3(3), 189-196.

Khan MH. 2007. “Anti-inflammatory Potential of Alkaloids as a Promising Therapeutic Modality”, Letters in Drug Design & Discovery (2017) 14, 240.

Kradin R. 2017. Chapter 3 – Pulmonary Responses to Injury, Understanding Pulmonary Pathology, Academic Press, 2017, Pages 21-36, ISBN 9780128013045,

Mohammed M, Osman W, Garelnabi E, Osman Z, Osman B, Khalid H, Mohamed M. 2014. Secondary metabolites as anti-inflammatory agents. Jphyto 3(4), 275-285.

Mosango DM. 2008. Peperomia pellucida (L.) PROTA (Plant Resources of Tropical Africa/ Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands.

Ooi DJ, Iqbal S, Ismail M. 2012. Proximate composition, nutritional attributes and mineral composition of Peperomia pellucida L. (Ketumpangan Air) grown in Malaysia. Molecules (Basel, Switzerland) 17(9), 11139-11145.

Osman NI, Sidik NJ, Awal A, Adam NA, Rezali NI. 2016. In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. Journal of Intercultural Ethnopharma Cology, 5(4), 343-349.

Pahwa R, Goyal A, Bansal P, et al. Updated 2020. Chronic Inflammation. StatPearls Publishing.

Raghavendra HI, Prashith TR. 2018. Ethnobotanical uses, phytochemistry and pharmacological activities of Peperomia pellucida (L.) Kunth (Piperaceae)- A review. International Journal of Pharmacy and Pharmaceutical Sciences 10. 1.

Rahman H, Eswaraiah MC, Dutta AM. 2015. In-vitro anti-inflammatory and anti-arthritic activity of Oryza Sativa Var. joha rice (an aromatic indigenous rice of Assam). Am Eurasian J Agric Environ Sci. 15, 115-21.

Sangeetha GM, Vidhya R. 2016. Invitro anti-inflammatory activity of different parts of Pedalium murex (L.). International Journal of Herbal Medicine 4, 31-36.

Souto AL, Tavares JF, da Silva MS, Diniz M, de Athayde-Filh PF, Barbosa Filho JM. 2011. Anti-inflammatory activity of alkaloids: an update from 2000 to 2010. Molecules (Basel, Switzerland) 16(10), 8515–8534.

Stone WL, Basit H, Burns B. Updated 2019. Pathology, Inflammation. StatPearls Publishing.

Tolentino RD, Tomas VC, Travezonda JC, Magnaye B. 2019. Herbal medicine utilization among Batanguenos. Asia Pacific Journal of Education, Arts and Sciences Vol. 6 No.1, 9-22