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Research Paper | September 1, 2015

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Selection of suitable housekeeping genes for gene expression study in caraway (Bunium persicum)

Davod Darvishi Zeidabadi, Mokhtar Jalali Javaran, Hamid Dehghani, Sajad Rashidi Monfared, Amin Baghizadeh

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J. Bio. Env. Sci.7(3), 50-58, September 2015

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Abstract

One of the most important issues in genetic engineering and molecular genetics is to study target gene expression that is aimed to quantify data resulted from measuring mRNA levels. Studying amount of gene expression by Real time PCR technique should normalize and compare the amount of mRNA in target gene to amount of mRNA in a housekeeping gene. The level of housekeeping gene expression is stable in different tissues and in all growth stages of an organism and it is not affected by experimental treatments. In each test of gene expression, several housekeeping genes should be studied in order to choose the best housekeeping gene for normalization of data resulted from the target gene expression. In the present study, stability of three housekeeping gene expressions, including actin-beta (ACT), ubiquitin (UBC) and elongation factor 1-alpha (EF1A) has been evaluated in germination, multi-leaf and flowering stages using real time PCR technique. Results obtained from data analysis via geNorm and Bestkeeper-1 software tools showed that EF1A gene is expressed more stable than two other genes and standard error (SE) is higher in the ACT and UBC genes and they are less expressed than EF1A. Concerning studies done on stability, uniformity and stability of EF1A gene expression in different ecotypes ofB. persicum is recommended to normalize data of the target gene expression.

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Selection of suitable housekeeping genes for gene expression study in caraway (Bunium persicum)

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