Sensitivity of Fusarium spp. infecting tomato in Khyber Pakhtunkhwa to thiophanate methyl

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Research Paper 01/06/2019
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Sensitivity of Fusarium spp. infecting tomato in Khyber Pakhtunkhwa to thiophanate methyl

Asma Akbar, Shaukat Hussain, Muhammad Fahim, Musharaf Ahmad, Zahir Shah
J. Bio. Env. Sci.14( 6), 136-151, June 2019.
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Abstract

Many constraints affect productivity and quality of tomato, the most important being an array of fungal and bacterial diseases. Fusarium wilt is present in all growing areas of KP, infecting tomato at different growth stages from vegetative growth up to fruit harvesting. This work aimed at determining the sensitivity of different pathogenic populations of F. equiseti, F. graminearum, F. solani and unknown Fusarium spp. isolates to thiophanate methyl an active ingredient in many broad spectrum fungicides used for Fusarium wilt of tomato. Fungal isolates were obtained from the diseased tomato collected in commercial fields at the Khyber Pakhtunkhwa, Pakistan. Twenty nine different isolates obtained from different areas were grown on PDA medium amended with increasing dosages and the effective concentration reducing the mycelial growth of isolates were determined.  From the results it was observed that isolates of F. equiseti was most resistant to the thiophanate methyl even at highest concentration with average percent inhibition 68. 49% followed by average percent inhibition 72.48% for F. solani. However, most sensitive strain was F. graminearum with average percent inhibition 45.42% even at lowest concentration. We reported that thiophanate methyl is an effective fungicide against F. equiseti, F. solani, F. graminearum and unknown spp. of Fusarium, associated with tomato wilt in Khyber Pakhtunkhwa Pakistan, however due to resistance in some isolates of F. equiseti the fungicide should be used in combination with other broad spectrum fungicide for management of the disease, to avoid resistance build up in pathogen population against the fungicide.

VIEWS 25

Agrios G. 2005. Plant diseases caused by fungi. Plant pathology, 4.

Akbar A, Hussain S, Ullah K, Fahim M, Ali GS. 2018. Detection, virulence and genetic diversity of Fusarium species infecting tomato in Northern Pakistan. PLoS One 13(9), e0203613.

Alexander NJ, Proctor RH, McCormick SP. 2009. Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium. Toxin Reviews, p.198-215.

Aoki T, O’Donnell K, Geiser DM. 2014. Systematics of key phytopathogenic Fusarium species: current status and future challenges. Journal of General Plant Pathology, p 189-201.

Benyephet Y, Shtienberg D. 1994. Effects of Solar-Radiation and Temperature on Fusarium-Wilt in Carnation. Phytopathology, p 1416-1421.

Biswas S, Pandey N, Rajik M. 2012. Inductions of defense response in tomato against Fusarium wilt through inorganic chemicals as inducers. Journal of Plant Pathology Microbiology.

Chehri K. 2016. Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran. Journal of Plant Protection Research, p 143-148.

Chehri K, Salleh B, Yli-Mattila T, ReddyK, Abbasi S. 2011. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi journal of Biological Sciences, p 341-351.

Choi YW, Hyde KD, Ho W. 1999. Single spore  isolation of fungi. Fungal Diversity.

Correll JC. 1991. The relationship between formae speciales, races, and vegetative compatibility groups in Fusarium oxysporum. Phytopathology, p 1061-1064.

Edel-Hermann V, Gautheron N, Steinberg C. 2012. Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathology, p 787-800. http://dx.doi.org/10.1111/j.1365-3059.2011.02551.

EdelHermann V, Gautheron N, Steinberg C. 2012. Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathology, p 787-800.

GordonT, Stueven M, Pastrana AM, Dennehy C, Kirkpatrick SC, Henry PM, Daugovish O. 2018. The effect of pH on spore germination, growth and infection of strawberry roots by Fusarium oxysporum f. sp. fragariae, cause of Fusarium wilt of strawberry. Plant Disease.

Häggblom P, Nordkvist E. 2015. Deoxynivalenol, zearalenone, and Fusarium graminearum contamination of cereal straw; field distribution; and sampling of big bales. Mycotoxin research, p 101-107.

Ignjatov M, Milosevic D, Nikolic Z, Gvozdanovic-Varga J, Jovicic D, Zdjelar G. 2015. Fusarium oxysporum as causal agent of tomato wilt and fruit rot.

James B, Godonou I, Atcha-Ahowe C, Glitho I, Vodouhe S, Ahanchede A, Goergen G. 2006. Extending integrated pest management to indigenous vegetables. Paper presented at the I International Conference on Indigenous Vegetables and Legumes. Prospectus for Fighting Poverty, Hunger and Malnutrition 752.

Jones JP, Woltz S. 1981. Fusarium-incited diseases of tomato and potato and their control. Fusarium: Diseases, Biology, and Taxonomy. The Penn. State Univ. Press, Univ. Park, PA, p 157-168.

Leslie JF, Summerell BA. 2008. The Fusarium laboratory manual: John Wiley Sons.

Massee G. 1895. The” sleepy disease” of tomatoes. Garden Chronicles Seriesp, 707-708.

Meletiadis J, Meis J, MoutonJW, Verweij PE. 2001. Analysis of growth characteristics of filamentous fungi in different nutrient media. Journal of clinical microbiology p 478-484.

Murad NBA, Kusai NA, Zainudin NAIM. 2016. Identification and diversity of Fusarium species isolated from tomato fruits. Journal of Plant Protection Research, p 231-236.

Nel B, Steinberg C, Labuschagne N, Viljoen A. 2007. Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana. Crop Protection p 697-705.

O’Donnell K, Ward TJ, Geiser DM, Kistler HC, Aoki T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal genetics and biology, p 600-623.

Ploetz RC. 2015. Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection, p 7-15.

Poddar R, Singh D, Dubey S. 2004. Management of chickpea wilt through combination of fungicides and bioagents. Indian Phytopathology, p 39-43.

Ramdial H, Hosein F, Rampersad S. 2016. First report of Fusarium incarnatum associated with fruit disease of bell peppers in Trinidad. Plant Disease, p 526-526.

Rozlianah F, Sariah M. 2006. Characterization of Malaysian isolates of Fusarium from tomato and pathogenicity testing. Research Journal of Microbiology, p 266-272.

Secor GA,Rivera VV. 2012. Fungicide resistance assays for fungal plant pathogens. In Plant Fungal Pathogensp, 385-392.

Soria S. Alonso R. Bettucci L. 2012. Endophytic bacteria from Pinus taeda L. as biocontrol agents of Fusarium circinatum NirenbergO’Donnell. Chilean Journal of Agricultural Research, p 281.

Toussoun TA, Nelson PE. 1968. A pictorial guide to the identification of Fusarium species according to the taxonomic system of Snyder and Hansen. A pictorial guide to the identification of Fusarium species according to the taxonomic system of Snyder and Hansen.

Woloshuk C P, Shim WB. 2013. Aflatoxins, fumonisins, and trichothecenes: a convergence of knowledge. FEMS Microbiol Rev, p 94-109. http://dx.doi.org/10.1111/1574-6976.12009

Xu S, Kim BS. 2016. Evaluation of Paenibacillus polymyxa strain SC09-21 for biocontrol of Phytophthora blight and growth stimulation in pepper plants. Tropical Plant Pathology, p 162-168.

Yeole G, Kotkar H, Teli N, Mendki P. 2016. Herbal Fungicide to control Fusarium Wilt in Tomato Plants. Biopesticides International, p 25-35.