Serological and Biochemical Based Identification of Vibrio Cholerae Isolated from Diarrheal Patients of Balochistan

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Research Paper 01/11/2021
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Serological and Biochemical Based Identification of Vibrio Cholerae Isolated from Diarrheal Patients of Balochistan

Ashiq Hussain, Muhammad Tahir Zehri, Abdul Sadiq, Muhammad Anwar Somro, Muhammad Kamran Taj, Sakina Khan, Umbreen Zafar, Nawab Khan Panezai, Saima Azam, Syeda Ayesha Ali
Int. J. Biosci.19( 5), 36-41, November 2021.
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Cholera, an acute diarrheal disease is a major public health problem in many developing countries including Pakistan. Vibrio cholerae is a causative agent of cholera disease. Over 200 serogroups of V. cholerae have been identified, but only O1 and O139 cause cholera epidemics. Present study was design to assess the routine laboratory diagnosis of V. cholerae strains by using biochemical and compared them with the serological identification (multi-serogroups or serotype) of V. cholerae. Total 1776 samples were collected from suspected cholera patients at different hospitals of the Balochistan from 2018 to 2020. All  isolates were examined and identified on the basis of colony characters on thiosulfate-citrate-bile salts-sucrose agar. Suspected colonies were subjected to gram staining, biochemical analysis and serological identification. Among the total samples (n=132;7.43%)  were positive V. cholerae from stool samples, futher identification was done through culture characteristic, microscopic examination and biochemical tests. While (n=121;91.6%) confirmed by serotyping using polyvalent antisera. All isolates were serogroup O1 ogawa. However, remaining (n=11; 8.3%) isolates were non-O1/non-O139 serogroups as they did not agglutinate with the polyvalent Inaba and Ogawa antisera and there were no isolates agglutinated with antisera O139 during this study. It is concluded that fecal specimens suspected for V. cholerae O1 and/or O139 should be confirmed by using traditional culture-based methods suitable for the isolation and identification of V. cholerae.


Azman AS, Rudolph KE, Cummings DA, Lessler J. 2013. The incubation period of cholera: a systematic review. Journal of Infection 66, 432-438.

Igbinosa EO, Okoh AI. 2008. Emerging Vibrio species: an unending threat to public health in developing countries. Research in Microbiology 159, 495-506.

Sack DA, Sack RB, Nair GB, Siddique AK. 2004. Cholera. Lancet 363, 223–233.

Tarh JE. 2020. A Review on diagnostic methods for the identification of Vibrio cholerae. Journal of Advances in Medicine and Medical Research 136-164.

Weil A, Midani F, Chowdhury F, Khan A, Begum Y, Charles R, Larocque R. 2015. The gut microbiome and susceptibility to Vibrio cholerae infection. In Open Forum Infectious Diseases. Infectious Diseases Society of America 2, 697.

Son MS, Megli CJ, Kovacikova G, Qadri F, Taylor RK. 2011. Characterization of Vibrio cholerae O1 El Tor biotype variant clinical isolates from Bangladesh and Haiti, including a molecular genetic analysis of virulence genes. Journal of Clinical Microbiology 49, 3739-3749.

Kirn TJ, Jude BA, Taylor RK. 2005. A colonization factor links Vibrio cholerae environmental survival and human infection. Nature 438, 863-866.

Goel AK, Jain M, Kumar P, Jiang SC. 2010. Molecular characterization of Vibrio cholerae outbreak strains with altered El Tor biotype from southern India. World Journal of Microbiology and Biotechnology 26, 281-287.

Kaper JB, Morris JR, JG, Levine MM. 1995. Cholera. Clinical Microbiology Reviews 8, 48-86.

Dua p, Karmakar A, Dutta K, Ghosh C. 2017. A simple procedure for isolation, identification and characterization of Vibrio cholerae from clinical samples. International Journal of Pharma and Bio Sciences 8, 57-64.

Choopun N, Louis V, Huq A, Colwell RR. 2002. Simple procedure for rapid identification of Vibrio cholerae from the aquatic environment. Applied and Environmental Microbiology 68, 995-998.

Rijal N, Acharya J, Adhikari S, Upadhaya BP, Shakya G, Kansakar P, Rajbhandari P. 2019. Changing epidemiology and antimicrobial resistance in Vibrio cholerae: AMR surveillance findings (2006–2016) from Nepal. BMC Infectious Diseases 19, 1-8.

Mahanta BN, Mahanta TG, Sinha R, Dutta A, Payeng D, Jawed Q. 2013. Investigation of a cholera outbreak in a tea garden of Sivasagar district of Assam. Indian Journal of Community Medicine 38, 240-243.

Maharjan R, Shrestha D, Acharya J, Adhikari N, Rijal KR, Ghimire P,  Shrestha UT. 2015. Change in biotype trend of Vibrio cholerae in Nepal. Med-Micro Journal of Microbiology 1, 46-54.

Pirkani GS, Iqbal M, Mohammad BA, Jogezai EK, Rasool G, Ud din N. 2005. Water born outbreak of Vibrio cholerae o1 ogawa. The Professional Medical Journal 12, 247-250.

Chander J, Kaistha N, Gupta V, Mehta M, Singla N, Deep A, Sarkar BL. 2009. Epidemiology & antibiograms of Vibrio cholerae isolates from a tertiary care hospital in Chandigarh, north India. Indian Journal of Medical Research 129, 613-617.