Int. J. Biosci.13( 2), 179-186, August 2018
Toxoplasmosis is one of the widely distributed zoonotic disease which is caused by an intracellular protozoan parasite Toxoplasma gondii. The objective of this study was to determine the seroprevalence and risk factors associated with infection in pregnant women of District Mardan, Khyber Pakhtunkhwa, Pakistan. Blood sample of 5mL was taken and the serum was tested for T. gondii IgG and IgM antibodies using Indirect Enzyme-Linked Immunosorbent Assay (ELISA) technique. Further information was collected by using preplanned questionnaire. A total of 1200 samples were tested for toxoplasmosis, out of which 39.5% were positive. Among seropositive pregnant women the seropositivity for IgG, IgM and both IgG and IgM antibodies was 43.03%, 40.5% and 16.45% respectively. The seropositivity was highest (58.18%) in age group 36-45 whereas lowest (33.92%) in age group 26-35 which was statistically significant. The findings of the present study suggest that T. gondii infection is widely spread in the study area which may constitute a significant risk for congenital defects, stillbirths and abortions during pregnancy in women. Screening for the infection among pregnant women can be done twice at least during pregnancy to protect both mother and child from toxoplasmosis. There should be proper awareness in the educational institutes as well as community awareness programs about the risk factors of toxoplasmosis.
Abamecha F, Awel H. 2016. Seroprevalence and risk factors of Toxoplasma gondii infection in pregnant women following antenatal care at Mizan Aman General Hospital, Bench Maji Zone (BMZ), Ethiopia. BMC infectious diseases 16, 460. https://doi.org/10.1186/s12879-016-1806-6.
Aldebert D, Hypolite M, Cavailles P, Touquet B, Flori P, Loeuillet C, Cesbron-Delauw MF. 2011. Development of high‐throughput methods to quantify cysts of Toxoplasma gondii. Cytometry Part A 79(11), 952-958. https://doi.org/10.1002/cyto.a.21138.
Aqeely H, El-Gayar EK, Perveen Khan D, Najmi A, Alvi A, Bani I, Mahfouz MS, Abdalla SE, Elhassan IM. 2014.Seroepidemiology of Toxoplasma gondii amongst pregnant women in Jazan Province, Saudi Arabia. Journal of tropical medicine 2014, 1-6. http://dx.doi.org/10.1155/2014/913950.
Ayi I, Edu S, Apea-Kubi K, Boamah D, Bosompem K, Edoh D. 2009. Sero-epidemiology of toxoplasmosis amongst pregnant women in the greater Accra region of Ghana. Ghana medical journal 43(3), 107-114. http://dx.doi.org/10.4314/gmj.v43i3.55325.
Bari A, Khan Q. 1990. Toxoplasmosis among pregnant women in northern parts of Pakistan. JPMA 40, 288-289.
Bjerke SEY, Vangen S, Holter E, Stray-Pedersen B. 2011. Infectious immune status in an obstetric population of Pakistani immigrants in Norway. Scandinavian journal of public health 39(5), 464-470.
Black MW, Boothroyd JC. 2000. Lytic cycle of Toxoplasma gondii. Microbiology and Molecular Biology Reviews 64(3), 607-623. DOI: 10.1128/MMBR.64.3. 607-623.2000.
Chaudhary Z, Ahmed R, Hussain S, Shakoori A. 2006. Detection of Toxoplasma gondii infection in butchers and buffaloes by polymerase chain reaction and latex agglutination test. Pakistan Journal of Zoology 38(4), 333-336.
Cook A, Holliman R, Gilbert R, Buffolano W, Zufferey J, Petersen E. 2000. Sources of toxoplasma infection in pregnant women: European multicentre case-control studyCommentary: Congenital toxoplasmosis-further thought for food. Bmj 321(7254), 142-147. DOI: https://doi.org/10.1136/bmj. 321.7254.142.
Da Silva MG, Vinaud MC, de Castro AM. 2015. Prevalence of toxoplasmosis in pregnant women and vertical transmission of Toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil, from 2012 to 2014. PLoS One 10(11), e0141700. https://doi.org/10.1371/journal.pone.0141700.
Dubey J, Jones J. 2008. Toxoplasma gondii infection in humans and animals in the United States. International journal for parasitology 38(11), 1257-1278. https://doi.org/10.1016/j.ijpara.2008.03.007.
Dubey JP. 2008. The history of Toxoplasma gondii-the first 100 years. Journal of Eukaryotic Microbiology 55(6), 467-475. https://doi.org/10.1111/j.1550-7408. 2008.00345.x.
Ferguson DJ. 2009. Toxoplasma gondii: 1908-2008, homage to Nicolle, Manceaux and Splendore. Memorias do Instituto Oswaldo Cruz Rio de Janeiro104(2),133-148. http://dx.doi.org/10.1590/ S0074 -02762009000200003.
Flatt A, Shetty N. 2012. Seroprevalence and risk factors for toxoplasmosis among antenatal women in London: a re-examination of risk in an ethnically diverse population. The European Journal of Public Health 23(4), 648-652. https://doi.org/10.1093/ eurpub/cks075.
Frimpong C, Makasa M, Sitali L, Michelo C. 2017. Seroprevalence and determinants of toxoplasmosis in pregnant women attending antenatal clinic at the university teaching hospital, Lusaka, Zambia. BMC infectious diseases 17(1), 10. https://doi.org/10.1186/s12879-016-2133-7.
Göz Y, Babür C, Aydin A, Kiliç S. 2007. Seroprevalence of toxoplasmosis, brucellosis and listeriosis in horses in Hakkari, eastern region of Turkey. Revue de Medecine Veterinaire 158(11), 534-539.
Hasan SF. 2011 Seroprevalence of toxoplasmosis among comers to marriage in Kerbala governorate. Kerbala Journal of Pharmaceutical Sciences 2, 97-102.
Hill D, Dubey J. 2002 Toxoplasma gondii: transmission, diagnosis and prevention. Clinical microbiology and infection 8(10), 634-640. https://doi.org/10.1046/j.1469-0691.2002.00485.x
Holzworth J. 1987. Diseases of the cat: Medicine and surgery. Philadelphia: Saunders.
Jones JL, Kruszon-Moran D, Wilson M, McQuillan G, Navin T, McAuley JB. 2001. Toxoplasma gondii infection in the United States: seroprevalence and risk factors. American journal of epidemiology 154(4), 357-365. https://doi.org/10. 1093/aje/154.4.357.
Kapperud G, Jenum PA, Stray-Pedersen B, Melby KK, Eskild A, Eng J. 1996. Risk factors for Toxoplasma gondii infection in pregnancy: results of a prospective case-control study in Norway. American journal of epidemiology 144(4), 405-412. https://doi.org/10.1093/oxfordjournals.aje.a008942.
Kijlstra A, Jongert E. 2009. Toxoplasma-safe meat: close to reality? Trends in parasitology 25(1), 18-22. https://doi.org/10.1016/j.pt.2008.09.008.
Lee S-E, Hong S-H, Lee S-H, Jeong Y-I, Lim SJ, Kwon OW. 2012. Detection of ocular Toxoplasma gondii infection in chronic irregular recurrent uveitis by PCR. The Korean journal of parasitology 50(3), 229-231. DOI: 10.3347/kjp.2012.50.3.229.
Liesenfeld O, Montoya JG, Kinney S, Press C, Remington JS. 2001. Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women: experience in a US reference laboratory. The Journal of infectious diseases 183(8), 1248-1253. https://doi.org/10.1086/319672.
Mwambe B, Mshana SE, Kidenya BR, Massinde AN, Mazigo HD, Michael D. 2013. Sero-prevalence and factors associated with Toxoplasma gondii infection among pregnant women attending antenatal care in Mwanza, Tanzania. Parasites & vectors 6(1), 222. https://doi.org/10.1186/1756-3305-6-222.
Negash T, Tilahun G, Medhin G. 2008. Seroprevalence of Toxoplasma gondii in Nazareth town, Ethiopia. East African Journal of Public Health 5(3), 211-214.
Nissapatorn V, Sawangjaroen N. 2011. Parasitic infections in HIV infected individuals: diagnostic & therapeutic challenges. The Indian journal of medical research 134(6), 878-897. doi: 10.4103/0971-5916.
Njunda AL, Assob JC, Nsagha DS, Kamga HL, Nde PF, Yugah VC. 2011. Seroprevalence of Toxoplasma gondii infection among pregnant women in Cameroon. Journal of public health in Africa 2(2).
Robert-Gangneux F, Dardé M-L. 2012. Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical microbiology reviews 25(2), 264-296. DOI: 10.1128/CMR.05013-11.
Sarkar MD, Anuradha B, Sharma N, Roy RN. 2012. Seropositivity of toxoplasmosis in antenatal women with bad obstetric history in a tertiary-care hospital of Andhra Pradesh, India. Journal of health, population, and nutrition 30(1), 87-92.
Shah M, Aftab MZ, Sthanadar A, Ali PA. 2014. Seroprevalence of Toxoplasma gondii Infection in Human Population of Mohmand Agency Khyber Pakhtunkhwa Pakistan.Pakistan Journal of Zoology 46(4).1169-1172.
Shah M, Zahid M, Asmat P, Alam A, Sthanadar A. 2013. Seroprevalence of Toxoplasma gondii in goats and sheep of district Mardan, Pakistan. International Journal of Biosciences 7(7), 90-97. doi: http://dx.doi.org/10.12692/ijb/3.7.90-97
Shah M, Zahid M, Sthanadar AA, Asmat P, Kausar A, Jan AH. 2013. Seroprevalence of Toxoplasma gondii infection in domestic animals of. Journal of Coastal Life Medicine 1(1), 70-73.
Sibley LD, Khan A, Ajioka JW, Rosenthal BM. 2009. Genetic diversity of Toxoplasma gondii in animals and humans.Philosophical Transactions of the Royal Society B: Biological Sciences 364(1530), 2749-2761. DOI: 10.1098/rstb.2009.0087.
Singh S. 2003. Mother-to-child transmission and diagnosis of Toxoplasma gondii infection during pregnancy. Indian journal of medical microbiology 21(2), 69-76.
Sroka J, Wójcik-Fatla A, Szymanska J, Dutkiewicz J, Zajac V, Zwolinski J. 2010. The occurrence of Toxoplasma gondii infection in people and animals from rural environment of Lublin region-estimate of potential role of water as a source of infection. Annals of Agricultural and Environmental Medicine 17(1),125-132.
Studeničová C, Benčaiová G, Holková R. 2006. Seroprevalence of Toxoplasma gondii antibodies in a healthy population from Slovakia. European journal of internal medicine 17(7), 470-473. DOI: https:// doi.org/10.1016/j.ejim.2006.07.007.
Tasawar Z, Aziz F, Lashari MH, Shafi S, Ahmad M, Lal V, Hayat CS. 2012. Seroprevalence of Human toxoplasmosis in southern Punjab, Pakistan. Pakistan Journal of Life and Social Scienes 10(1), 48-52.
Wam EC, Sama LF, Ali IM, Ebile WA, Aghangu LA, Tume CB. 2016. Seroprevalence of Toxoplasma gondii IgG and IgM antibodies and associated risk factors in women of child-bearing age in Njinikom, NW Cameroon. BMC research notes 9, 406. https://doi.org/10.1186/s13104-016-2206-0.