Seroprevalence and Risk Factors of Hepatitis C Virus (HCV) in Tehsil Takht Bhai District Mardan, KPK, Pakistan

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Research Paper 01/05/2018
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Seroprevalence and Risk Factors of Hepatitis C Virus (HCV) in Tehsil Takht Bhai District Mardan, KPK, Pakistan

Mudassir Khan, Fazal Jalil, Misbahud Din, Sajid Ali, Aziz Ahmad
Int. J. Biosci.12( 5), 247-252, May 2018.
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The assessment of Hepatitis C Virus based on seroprevalence with respect to its risk factors is carried out amongst the volunteers of Tehsil Takht Bhai district Mardan, Khyber Pakhtunkhwa, Pakistan. This type of study has never been carried out here in this region so far, so we done this research study to explore the prevalence ratio of HCV in this region. We used HCV Ab rapid test cassette serum/plasma (USA) kit to detect the antibodies produce by immune system in reaction to HCV in the serum/plasma of volunteers. Then ELISA was carried out for the ICT positive individuals and hence finally ELISA positive samples were reconfirmed by RT-PCR for Hepatitis C Virus. In every case of HCV socioeconomic details were noted apparently amongst the victims. The study population comprised of 640 volunteers, 385 (60.2%) males and 255 (39.8%) females. The overall seroprevalence was found to be 4.69% of HCV in Tehsil Takht Bhai district Mardan. This seroprevalence ratio was 5.19% in males and 3.92% in females. ICT positive samples were then refined by 3rd generation ELISA which indicated that 86.67% of the ICT positive volunteers had antibodies against HCV. Moreover to confirm the active infection, ELISA positive samples were investigated by real time PCR which showed that 65.38% of the ELISA positive volunteers had HCV RNA in their blood. Significant risk factors for acquiring HCV infection include contaminated reused syringes/blades, blood transfusion, surgical operations and dental procedures. Body weakness was common symptom in HCV positive volunteers. Generally we can protect ourselves from Hepatitis C Virus via blood screening before donation and to avoid injecting equipment i-e needles etc, sterilizing surgical equipments and following safe sexual activities.


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