Software Simulator Bioprocess (SSBP) to estimate hydrodynamic stress conditions in cell cultures performed in shaking bioreactors

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Research Paper 01/03/2017
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Software Simulator Bioprocess (SSBP) to estimate hydrodynamic stress conditions in cell cultures performed in shaking bioreactors

César Reyes Reyes, Hebert Jair Barrales-Cureño, Petra Andrade Hoyos, Alfonso Luna-Cruz, Ketzasmin Armando Terrón-Mejía, Luis Germán López-Valdez, Leticia Mónica Sánchez-Herrera, Juan Antonio Cortes-Ruíz, María Carmina Calderon-Caballero, Jordi Orlando González Osuna, Salvador Chávez-Salinas
Int. J. Biosci.10( 3), 143-156, March 2017.
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In order to estimate hydrodynamic stress conditions in cultures carried out in shake flasks we used a Software (SSBP). The systematic analysis was performed with mammal cells, plant cells, filamentous molds, protozoa and bacteria; all data were collected from the literature. The parameters more useful for the quantification of hydrodynamic stress was the energy dissipation threshold and critical length eddy. According to these parameters the mammal cell line AGE.1.HM®, Rubia tinctorium, Penicillium purpurogenum, Streptomyces zaomyceticus, bacteria on their capacity to colony forming units (FCU) and Trichoderma harzianum were the most tolerant to hydrodynamic stress in shake flask cultures. In counterpart, erythrocytes, CHO cells, Penicillium citrinum, Ceratocorys horrida and Protoceratium reticulatum were the most susceptible. The use of shear stress and shear rate to compare hydrodynamic stress, at least in shake flask cultures, cannot be useful when compares different biological systems.  In this context, the Kolmogoroff theory, neither applied at all in shaking bioreactors. Finally, the highest exposition to hydrodynamic stress in terms of  the maximum drop diameter in shake flask cultures corresponds to protozoan Protoceratium reticulatum, Lactococcus lactis, mammal cell line AGE.1.HM, mollusk larvae Dreissena polymorpha and Rubia tinctorium although with great exposition differences to energy dissipation thresholds.


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