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Somatic embryogenesis and plant regeneration capacity from mature and immature durum wheat embryos

El Houssine Bouiamrine, Mohamed Diouri, Rachid EL Halimi

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Int. J. Biosci.2(9), 29-39, September 2012


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Seven Moroccan durum wheat genotypes were tested on callus induction and plantlet regeneration from immature and mature embryos cultures. The genotypes tested were: Karim, Sebou, Isly, Jawhar, Anouar, Ourgh and Massa. For all the cultivars, five parameters were considered: Callogenesis, zygotic germination, Callus growth, percentage of embryogenic calli, percentage of regeneration and Number of plantlets per regenerating callus (NPRC). The results of the study showed that morphogenetic capacity of callus was influenced by the genotype and explant sources. Immature embryos gave much higher levels of embryogenic callus and plant regeneration than mature embryos. The highest regeneration capacity was observed from immature embryos in Anouar, Karim, Ourgh and Sebou. Therefore, they could be used in in vitro selection. Among the regenerated plants, albino plants have been observed from immature embryo calluses. Other variations were also observed after transfer of plantlets to soil such as abnormalities in spike morphology and leaf coloration.


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Somatic embryogenesis and plant regeneration capacity from mature and immature durum wheat embryos

Abumhadi N, Kamenarova K, Todorovska E, Stoyanova M, Dimov G, Todorovska E, Trifonova A, Gecheff K, Atanassov A. 2005. Biotechnological approaches for cereal crops improvement. Part I: Development of in vitro culture and genetic transformation technologies in cereals. Biotechnology & Biotechnological Equipment 19, 72-90.

Ahloowalia BS. 1982. Plant regeneration from callus culture in wheat. Crop Science 22, 405-410.

Bapat SA, Joshi CP, Mascarenhas AF. 1988. Occurrence and frequency of precocious germination of somatic embryos is a genotype-dependent phenomenon in wheat. Plant cell Reports 7, 538-541.

Benderradji L, Brini F, Kellou K, Ykhlef N, Djekoun A, Masmoudi K, Bouzerzour H. 2012. Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions. ISRN Agronomy. Vol. 2012, Article ID 367851.

Benkirane H, Sabounji K, Chlyah A, Chlyah H. 2000. Somatic embryogenesis and plant regeneration from fragments of immature inflorescences and coleoptiles of durum wheat. Plant Cell,Tissue and Organ Culture 61, 2107-113.

Bennici A, Caffaro L, Dameri RM, Gastaldo P, Profumo P. 1988. Callus formation and plantlet regeneration from immature Triticum durum. Euphytica 39(3), 255-263.

Bouiamrine EH, Mzouri K, Amssa M. 1999. Effet du génotype et du milieu de culture dans la culture d’embryons immatures de blé dur (Triticum durum Desf.) et de blé tendre (Triticum aestivum L.). In:Aupelf-Uref ed.. Actualités Scientifiques, Biotechnologies, amélioration des plantes et Sécurité alimentaire. Estem, Paris, 533-534. (In French).

Bregitzer P, Campbell RD. 2001. Genetic markers associated with green and albino plant regeneration from embryogenic barley. Crop Science 41, 173-179.

Chevrier N, Quoreshi JA, Hull P, Kartha KK. 1990. Heritability of in vitro regeneration in wheat (T. aestivum L.). Canadian Journal of Plant Science 70, 547-550.

Chlyah H, Hsaine M, Karim R, Chlyah A. 1990. Improvement of somatic embryogenesis in wheat by segmentation of cultured embryos. In: Bajaj YPS.,ed. Biotechnology in agriculture and forestry.Vol. 13. Wheat. SpringerVerlag, Heidelberg, Germany. Springer-Verlag, 88-97.

Chowdhury SH, Kato K, Yamamato Y, Hayashi K. 1991. Varietal variation in plant regeneration capacity from immature embryo among common wheat cultivars. Japanese Journal of Breeding 41, 442-450.

De Buyser J, Marcotte JL, Henry Y. 1992. Genetic analysis of in vitro wheat somatic embryogenesis. Euphytica 63, 265-270.

Farshadfar E, Elyasi P, Aghaee M. 2012. In vitro selection for drought tolerance in common wheat (Triticum aestivum L) Genotypes by Mature Embryo Culture. American Journal of Scientific Research 48, 102-115.

Galović V, Rausch T, Gršić-Rausch S. 2010. Mature embryo-derived wheat transformation with major stress modulated antioxidant target gene. Archives of Biological Sciences, Belgrade 62 (3), 539-546.

Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA. 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cellular and Developmental Biology-Plant 32, 272-289.

He GY, Lazzeri PA. 2001. Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures. Euphytica 119, 369-376.

Hess JR, Carman JG. 1998. Embryogenic competence of immature wheat embryos: genotype, donor plant environment, and endogenous hormone levels. Crop Science 38, 249-253.

Ghaemi, M, Sarrafi A. 1994. The effect of the ‘D’ genome from synthetic wheat lines in anther culture response. Plant Breeding 112, 76-79.

Gonzalez JM, Friero E, Jouve N. 2001. Influence of genotype and culture medium on callus formation and plant regeneration from immature embryos of Triticum turgidum Desf. Cultivars. Plant Breeding 120, 513–517.

Hafeez I, Sadia B, Sadaqat HA, Kainth RA, Iqbal MZ, Khan IA. 2012. Establishment of efficient in vitro culture protocol for wheat land races of Pakistan. African Journal of Biotechnology 11(11), 2782-2790.

Higgins P, Mathias RJ. 1987. The effect of the 4 B chromosomes of hexaploid wheat on the growth and regeneration of callus cultures.Theoretical and Applied Genetics 74, 439-444.

Khanna HK, Raina SK. 1998. Genotype x culture media interaction effects on regeneration response of three indica rice cultivars. Plant Cell Tissue and Organ Culture 52(3), 145-153.

Larkin PJ, Scowcroft WR. 1981. Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics 60, 197-214.

Maddock SE, Lancaster VA, Risiott R, Franklin J.1983. Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum L.). Journal of Experimental Botany 34, 915-926.

Malik ST, Hamid R, Tayyaba Y, Minhas NM. 2003. Effects of 2,4-D on callus induction from mature wheat (Triticum aestivum L.) seeds. International Journal of Agriculture and Biology 6, 156-159.

Malik SI, Rashid H, Yasin T, Minhas NM. 2004. Plant regeneration by somatic embryogenesis from callus of mature seed explants of bread wheat (Triticum aestivum L.). Pakistan Journal of Botany 36, 629-634.

Manivannan A, Kaul J, Singode A, Dass S. 2010. Callus induction and regeneration of elite Indian maize inbreds. African Journal of Biotechnology 9(44), 7446-7452.

Mathias RJ, Simpson ES. 1986. The interaction of genotype and culture medium on tissue culture response of wheat (Triticum aestivum L.) callus. Plant Cell,Tissue and Organ Culture 7, 31-37.

Mzouri K, Amssa M, Bouiamrine EH. 2001. Somatic embryogenesis from immature embryos of wheat cultivars (Triticum aestivum L.): genotype effect. Acta Botanica Gallica 148(3), 215-225.

Mzouri K, Amssa M. 2002a. Amélioration de l’embryogenèse somatique à partir d’embryons immatures chez le Blé tendre (Triticum aestivum L.). I – Effet du stade de développement des embryons. Acta Botanica Gallica 149(2), 179-188. (In French).

Mzouri K, Amssa M. 2002b. Amélioration de l’embryogenèse somatique à partir d’embryons immatures chez le Blé tendre (Triticum aestivum L.). II : Effet des régulateurs de croissance sur la callogenèse. Acta Botanica Gallica 149(4), 357-368. (In French).

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.

Nabors MW, Heyser JW, Dykes TA, DeMott KJ. 1983. Long-duration, high-frequency plant regeneration from cereal tissue cultures. Planta 157, 385-391.

Nasircilar AG, Turgut K, Fiskin K. 2006. Callus induction and plant regeneration from mature embryos of different wheat genotypes. Pakistan Journal of Botany 38, 637-645.

Ombori O, Gitonga NM, Machuka J. 2008. Somatic embryogenesis and plant regeneration from immature embryos of tropical maize (Zea mays L.) inbred lines. Biotechnology 7(2), 224-232.

Özgen M, Turet M, Ozcan S, Sancak C.1996. Callus induction and plant regeneration from immature and mature embryos of winter durum wheat genotypes. Plant Breeding 115(6), 455-458.

Özgen M, Turet M, Altýnok S, Sancak C. 1998. Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) Genotypes. Plant Cell Reports 34(2), 331-335.

Ozias-Akins P, Vasil IK 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): evidence for somatic embryogenesis. Protoplasma 110, 95-105.

Ozias-Akins P, Vasil IK. 1983. Callus induction and growth from the mature embryo of T. aestivum (wheat) embryos. Protoplasma 115, 104-113.

Qureshi JA, Kartha KK, Abrams SR, Steinhauer L. 1989. Modulation of somatic embryogenesis in early and late-stage embryos of wheat (Triticum aestivum L.) under the influence of (plus orminus) abscisic acid and its analogs. Plant Cell Tissue and Organ Culture 18, 55 – 69.

R Development Core Team. 2012. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL

Rahman MM, Shamsuddin AKM, Asad U. 2008. In vitro regeneration from mature embryos in spring wheat. International Journal of Sustainable Crop Production 3(2), 76-80.

Redway FA, Vasil V, Lu D, Vasil IK. 1990. Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Theoretical and Applied Genetics 79, 609-617.

Ren J, Wang X, Yın J. 2010. Dicamba and Sugar Effects on Callus Induction and Plant Regeneration from Mature Embryo Culture of Wheat. Agricultural Sciences in China 9 (1), 31-37.

Sharma VK, Hansch R, Mendel RR, Schulze J. 2005. Mature embryo axis based high frequency somatic embryogenesis and plant regeneration from multiple      cultivars of barley (Hordeum vulgare L.). Journal of Experimental Botany 56 (417), 1913-1922.

Šerhantová V, Ehrenbergerová J, Ohnoutková L. 2004.Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic. Plant, Soil and Environment 50 (10), 456-462.

Tyankova ND, Zagorska N, Chardakov V, Dryanova A, Dimitrov B. 2006. Chromosomal Effects on In Vitro Morphogenesis in Wheat Intervarietal Substitution Lines. Czech Journal of Genetics and Plant Breeding 42(1), 15–19.

Vasil V, Vasil IK. 1981. Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum and P. americanum x P. purpureum hybride. American Journal of Botany 68, 864-872.

Vendruscolo ECG, Schuster I, Negra ES, Scapim CA. 2008. Callus induction and plant regeneration by Brazilian new elite wheat genotypes. Crop Breeding and Applied Biotechnology 8, 195-201.

Yu H, Wang W, Wang Y, Hou B. 2012. High frequency wheat regeneration from leaf tissue explants of regenerated plantlets. Advances in Bioscience and Biotechnology 3, 46-50.

Zamora AB, Scott KJ. 1983. Callus formation and plant regeneration from wheat leaves. Plant Science Letters 29, 183-189.


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