Somatic embryogenesis from leaf & petiole explants of some Rosa hybrida L. cultivars

Paper Details

Research Paper 01/12/2014
Views (344) Download (3)
current_issue_feature_image
publication_file

Somatic embryogenesis from leaf & petiole explants of some Rosa hybrida L. cultivars

Behrooz Pirniakan, Siamak Kalantari, Mesbah Babalar
Int. J. Biosci.5( 11), 1-7, December 2014.
Certificate: IJB 2014 [Generate Certificate]

Abstract

In this study, somatic embryogenesis of four Rosa hybrida L. cultivars, including BlackMagic, HotLady, Audio and Eldorado, from leaf and petiole explants in invitro condition was investigated. Explants were taken in April and cultivated in MS medium. A combination of 2,4-D+BA and NAA+KIN were applied for callus induction. For somatic embryogenesis the ½ MS medium containing hormonal combination of 2,4-D, BA, NAA, KIN and GA3 were used. The highest callus production was obtained from petiole explant, Audio cultivar and hormonal combination of NAA+KIN. The highest and lowest percentage of embryogenesis were manifested in HotLady and Eldorado cultivars, respectively. A hormonal treatment consisting of 0.5mg/l BA+0.3mg/l 2,4-D led to the highest rate of embryogenesis. Petiole explants led to a statistically significant (p<0.05) greater somatic embryogenesis.

VIEWS 4

Davis P. 1995. The hormones: their nature, occurrence, and functions. In: Davis PJ, (ed). Plant Hormones : physiology, Biochemistry, and Molecular Biology. Kluwer Academic Publishers, Dordrecht, 1-12 p.

De Wit JC, Esendam HF, Honkanen JJ, Tuominen U. 1990.  Somatic  embryogenesis  and regeneration of flowering plants in rose. Plant Cell Reports 9, 456 – 458. http://dx.doi.org/10-1007/BF00232272.

Estabrooks T, Browne R, Dong Z. 2006. 2, 4, 5-Trichlorophenoxyacetic acid promotes somatic embryogenesis in the rose cultivar ‘Livin Easy’ (Rosa sp.). Plant Cell Reports. 26, 153-160. http://dx.doi.org/10-1007/s00299-006-0231-5

Hsia C, Korban SS. 1996. Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima Plant-Cell Tiss Org Cult 44,1-6.

Kim ChK, Chung JD, Jee SO, Oh JY. 2003. Somatic Embryogenesis from In Vitro Grown Leaf Explants of Rosa hybrida L. J. Plant Biotechnology 5, 169 ~ 172 p.

Kintzios S, Manos C, Makri O. 1999. Somatic embryogenesis from mature leaves of rose (Rosa sp.). Plant Cell Reports 18, 467 – 472.

Kunitak H, Imamizo H, Mil H. 1993. Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thumb.). Plant Sci 90, 187-194.

Marchant R, Davey MR, Lucas JA, Power JB. 1996. Somatic embryogenesis and plant regeneration in floribunda rose (Rosa hybrida L. cvs. Trumpeter and Gala Tidings). Plant Sci 120, 95-105. http://dx.doi.org/10.1016/S01689452(96)04479-2

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Plant Physiol 15, 473-497. http://dx.doi.org/10.1111/j.13993054.1962.tb08052.x

Murali S, Sreedhar D, Lokeswari TS. 1996. Regeneration through somatic embryogenesis from petal-derived calli of Rosa Hybrida L.Arizona(hybrida tea). Euphytica 91, 271-275.

Noriega C, Sondhl MR. 1991. Somatic embryogenesis in hybrid tea roses. Biotechnology 9, 991-993. http://dx.doi.org/10.1038/nbt1091-991

Roberts AV, Yokoya K, Walker S, Mottley J. 1995. Somatic embryogenesis in Rosa spp. In: Jain S, Gupta P, Newton R (Eds.), Somatic Embryogenesis in Woody Plants, Vol 2 Kluwer, The Netherlands, 227-289 p.

Rout GR, Debata BK, Das P. 1991. Somatic embryogenesis in callus cultures of Rosa hybrid acv. Landora. Plant Cell Tissue Org Cult 27, 65-69.

Sarasan V, Roberts AV, Rout GR. 2001. Methyl laurate and 6-benzyladenine promote the germination of somatic embryos of a hybrid rose. Plant Cell Rep 20, 183-186. http://dx.doi.org/10.1007/s002990000303

Visessuwan R, Kawai T, Mii M. 1997. Plant regeneration systems from leaf segment culture through embryogenic callus formation of Rosa hybrida and R.canina. Breed Sci 47, 217-222.

Xiangqian Li, Sergei F, Krasnyanski, Schuyler S, Korban. 2002. Somatic embryogenesis, secondry somatic embryogenesis, and shoot organogenesis in Rosa. J. Plant Physiol. 159, 313-319. http://dx.doi.org/10.1078/0176-1617-00688