Spectrophotometric determination of Phosphate in water and sugarcane sample

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Research Paper 01/08/2021
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Spectrophotometric determination of Phosphate in water and sugarcane sample

Nuzhat Arshad, Anjum Ayub, Mahnoor Nafees, Ahmed Abdullah, Zain Sultan
J. Biodiv. & Environ. Sci. 19(2), 111-115, August 2021.
Copyright Statement: Copyright 2021; The Author(s).
License: CC BY-NC 4.0

Abstract

This study is aimed to evaluate the phosphate concentration in parts per million in water and two sugarcane samples from two different Field areas through molybdenum blue phosphorous method in conjugation with UV-Visible spectrometer. Phosphomolybdate complex was formed by the addition of moly date and subsequently reduced with hydrazine hydrate. This obeys Beers-Lamberts law at 840 nm in 0.1-0.7 ppm concentration range. The reagents used are potassium hydrogen phosphate, ammonium moly date and hydrazine sulphate. The amount of phosphate present in water and sugarcane is found to be proportional to the color intensity of reduced phosphomolybdate solution. The phosphate concentration in Malir water sample is 0.427 ppm, in sugar can samples from Chakwal and Larkana district of Pakistan are 0.847 and 0.671 ppm, respectively.

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Ganesh S, Khan F, Ahmed MK, Velavendan P, Panday NK, Mudali UK. 2012. Spectrophotometric determination of trace amounts of phosphate in water and soil. Water Science & Technology 66, 2653-2658. https://doi.org/ 10.2166/wst.2012.468

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Lal KN, Singh JN. 1961. Uptake of phosphorus and its accumulation in component parts of sugarcane as affected by age, phosphorus deficiency and levels of phosphorus. Soil and Plant Food 6, 120-126. https://doi.org/10.1080/00380768.1961.10430937

Mahadevaiah MS, Yogendra K, Mansour SAG, Suresha MS, Satish MA, Nagendrappa G. 2007. A simple Spectrophotometric Determination of Phosphate in Sugarcane Juices, Water and Detergent Sample. E-Journal of Chemistry 4, 467-473. https://doi.org/10.1155/2007/576560

Nur Habibah, Dhayanaputri IGAS, Karta, Sandari CDWH, Hadimc IW. 2018. A Simple Spectrophotometric Method for the Quantitative Analysis of Phosphate in the Water Samples. Jurnal Sains dan Teknologi 7, 1-7. http://dx.doi.org/10 .23887/jst-undiksha.v7i2.13940

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Pradhan S, Pokhrel MR. 2013. Spectrophotometric determination of phosphate in sugarcane juice, fertilizer, detergent and water samples by molybdenum blue method. Scientific World 11, 1-5. https://doi.org/10.3126/sw.v11i11.9139

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