Paper Details

Spectroscopic studies on the interaction between acridine-spermine conjugate with DNA

Masoud Karimi Goftar, Narges Alizadeh Rayeni, Neda Mohamadi

DOI: http://dx.doi.org/10.12692/ijb/5.4.27-33

Int. J. Biosci. 5(4), 27-33. August, 2014. (PDF)

Abstract:

Interaction of acridine-spermine with chicken blood ds-DNA has been investigated via spectrophotometric method at different temperatures. The equilibrium constants of acridine-DNA complex in several temperatures have been determined by applying MCR-ALS method on the absorption spectra. The quantitative analysis of the data of undefined mixtures, was carried out by simultaneous resolution of the overlapping spectral bands in the whole set of absorption spectra. The enthalpy and entropy of the dimerization reactions were determined from the dependence of the equilibrium constants on the temperature (van’t Hoff equation). From these results it can be inferred that the driving force of the equilibrium is of enthalpic origin.