Spore germination of silver fern (Pityrogramma calomelanos (L.) Link) using various lead (Pb) concentrations

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Research Paper 01/07/2018
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Spore germination of silver fern (Pityrogramma calomelanos (L.) Link) using various lead (Pb) concentrations

Cherie Cano-Mangaoang, Florfe M. Acma
J. Bio. Env. Sci.13( 1), 84-91, July 2018.
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Utilization of spores to screen potential of certain species of ferns is not new in the field of phytoremediation. However, for Pityrogramma calomelanos this is not yet done thus, in this study spores were used to investigate capability of this species to tolerate lead. This species has been documented to hyperaccumulate arsenic in the environment hence its capacity to accumulate other heavy metals is of interest. This study aims to assess the spore germination of silver fern (P. calomelanos) exposed to different concentrations of lead nitrate. Spores were collected by scraping the sori and sowed in sterile soil with various lead nitrate concentrations- T0-control, T1-100ppm, T3-150ppm and T3-200ppm.  Result of the study showed that P. calomelanos spores germinated in all treatments. Germination of spores occur 7 days after sowing both in control and in various concentration of lead nitrate used. However, at T3 fewer rhizoids developed and a smaller prothallus was observed. Prothallial development is normal in all treatments exhibited by multiseriate and green in color similar to the control. Our result indicate that P. calomelanos may be a potential accumulator of lead. Utilization of spore in screening potential of plant species in phytoremediation could lessen the use of mature fern plant.  This is a practical strategy in any conservation effort involving ferns species utilized in the field of phytoremediation.


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