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Stability analysis of candidate bollgard bt cotton (Gossypium hirsutum L.) genotypes for yield traits

Research Paper | November 1, 2018

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Muhammad Zaffar Iqbal, Shahid Nazir, Sajid-ur-Rahman, Muhammad Younas

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Int. J. Biosci.13( 5), 55-63, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.55-63


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During varietal development process, multi-location trials are conducted to evaluate the performance of new cotton lines for yield potential and stability. Multi-location trials consisting of 89 candidate cotton genotypes were carried out at 10 locations under different agro-climatic zones. Presence of Cry1Ac gene of Mon-531 event was verified using isolated DNA and event-specific primers in PCR. Toxic cry protein was identified using qualitative strip test from ten randomly selected plants. To assess genotype by environment interaction and to evaluate the stability and adaptability, data were analyzed using GGE-biplot approach. Two mega environments were found and Ghotki (SG) was ideal location with maximum discriminative and representative properties. Genotype, MNH-1026 (1) performed best in all locations and proved to be an ideal genotype with maximum stability and adoptability followed by GH-Deebal (2). Hence, this information will be very useful for cotton breeders who intend to develop high yielding, widely adopted and stable genotypes, and be helpful for variety registration/approval departments for giving general and specific recommendations.


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Stability analysis of candidate bollgard bt cotton (Gossypium hirsutum L.) genotypes for yield traits

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