Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | September 1, 2013

VIEWS 3
| Download

Stimulating effects of hydro-ethanolic and acetonic extracts of Phaseolus vulgaris (Fabaceae) on proliferation and differentiation of osteoblastic cells

Kouakou Koffi, Egrise Dominique, Kati-Coulibaly Séraphin, et Moreno-Reyes Rodrigo

Key Words:


Int. J. Biosci.3(9), 185-194, September 2013

DOI: http://dx.doi.org/10.12692/ijb/3.9.185-194

Certification:

IJB 2013 [Generate Certificate]

Abstract

Extracts from Phaseolus vulgaris (Fabaceae) were studied for their potential effects on the metabolism of osteoblastic cells. Results indicate that the hydro-ethanolic and acetonic extract stimulates the proliferation of ROS cells. The stimulative effects induced by hydro-ethanolic extract persist even in the presence of an inhibitor of estrogenic receptor such as Tamoxifen. The addition of Tamoxifen in the culture medium inhibits the effects induced by acetonic extract of Phaseolus vulgaris for 10-6 and 10-5 g/ml doses of the extract. For higher doses of extract such as 10-4 and 10-3 g/ml, the stimulative effects of acetonic extract is not affected by Tamoxifen. When the Tamoxifen is added to the media before extracts, effects induced by plant extracts persist only at 10-3 g/ml. The effects disappear at 10-6, 10-5 and 10-4 g/ml. The hydro-ethanolic extract stimulates the differentiation of osteoblastic ROS cells whereas the acetonic extract has no such effect. The effects induced by the hydro-ethanolic extract disappear in the presence of Tamoxifen. Our results indicate that extracts of Phaseolus vulgaris stimulate both the proliferation and the differentiation of osteoblastic cells. These actions could be due, among other reasons, to phyto-estrogens in the extract acting, at least partially, on the estrogens receptors. These molecules may also act through mechanisms where estrogen receptors are not involved. Our results suggest the presence of non-estrogenic substances and/or acting through mechanisms different from those of the estrogen receptors.

VIEWS 3

Copyright © 2013
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Stimulating effects of hydro-ethanolic and acetonic extracts of Phaseolus vulgaris (Fabaceae) on proliferation and differentiation of osteoblastic cells

Adlercreutz H. 1998. Evolution, nutrition, intestinal microflora and prevention of cancer: a hypothesis. Proceedings of the Society of Experimental Biology and Medicine 217, 241-247.

Arjmandi BH, Khalil DA., Smith BJ, Lucas EA, Juma S, Payton ME, WILD RA. 2003. Soy protein has a greater effect on bone in postmenopausal women not on hormone replacement therapy, as evidenced by reducing bone resorption and urinary Calcium excretion. The Journal of Clinical Endocrinology and Metabolism 88(3), 1048–1054. http://dx.doi.org/10.1210/jc.2002-020849

Benié T, Thieulant M-L. 2003. Interaction of some traditional plant extracts with uterine estrogen or progestin receptors. Phytotherapy research 17, 756-760. http://dx.doi.org/10.1002/ptr.1208

Boue SM, Wiese TE, Nehls S, Burrow ME, Elliott S, Carter-Wientjes CH, Shih BY, Maclachlan JA, Cleveland TE. 2003. Evaluation of the estrogenic effects of legume extracts containing phytoestrogens. Journal of Agricultural Food Chemistry 51. 2193-2199. http://dx.doi.org/10.1021/jf021114s

Chen X, Anderson JJB. 2002. Effects of isoflavones on osteoblast proliferation and differentiation. Journal of Nutrition 132, 616S

Choi EM, Suh KS, Kim YS, Choue RW, Koo SJ. 2001. Soybean ethanol extract increases the function of osteoblastic MC3T3-E1 cells. Phytochemistry 56, 733-739. http://dx.doi.org/10.1016/s0031-9422(00)00484-2

Choi EM. 2005. The licorice root derived isoflavan glabridin increases the function of osteoblastic MC3T3-E1 cells. Biochemical Pharmacology 45, 698-704.

Cho Y, Chung S, Cho K. 2002. Black bean (Rhynchosia molubilis, Yak-kong) exerts a prominent estrogenic effect on the proliferation of human MG63 osteoblastic cells. Journal of Nutrition 132, 614-s

Dang ZC, Papapoulos S, Löwik C. 2002. Phyto-estrogens enhance osteogenegis and concurrently inhibit adipogenesis. Journal of Nutrition 132, 617-s.

Draper R, Edel M, Dick I, Randall A, Martin G, Prince R. 1997. Phytoestrogens Reduce Bone Loss and Bone Resorption in Oophorectomized Rats1. American Society for Nutritional Sciences, 1795-1799.

De wilde A, Colin C, Lacroix H. 2001. In vitro and ex vivo effects of an isoflavone-rich diet on bone formation in growing pigs. Annal of Nutrition Metabplism 45, 227-S.

Duque A, Rakic P. 2011. Different effects of bromodeoxyuridine and 3H-thymidine incorporation into DNA on cell proliferation, position and fate. The journal of Neurosciences 31(42), 15205-15217. http://dx.doi.org/10.1523/jneurosci.3092-11.2011

Egrise D, Martin D, Neve P, Verhas M, Schoutens A. 1990. Effects and interactions of 17β-estradiol, T3 and 1,25(OH)2D3 on cultured osteoblasts from mature rats. Bone and mineral 11, 273-283.

Egrise D, Martin D, Neve P, Vienne A, Verhas M, Schoutens A. 1992. Bone blood flow and In vitro proliferation of bone marrow and trabecular bone osteoblast-like cells in ovariectomized rats. Calcified Tissue International 50, 336-341.

Janeczko A, Skoczowski A. 2005. Mammalian sex hormones in plants. Folia Histochemica et Cytobiologica 43(2), 71-79.

Jeong J-C, Lee JW, Yoon CH, Lee YC, Chung KH, Kim MG, Kim CH. 2005. Stimulative effects of Drynariae rhyzoma extracts on the prolifeation and differentiation of osteoblastic MC3T3-E1 cells. Journal of Ethnopharmacology 96(3), 489-495. http://dx.doi.org/10.1016/j.jep.2004.09.038

Kanno S, Hirano S, Kayama F. 2004. Effects of phytoestrogens and environmental estrogens on osteoblastic differentiation MC3T3-E1 cells. Toxicology 196, 137-145. http://dx.doi.org/10.1016/j.tox.2003.12.002

Kelly GE, Joannou GE, Reeder AY, Nelson C, Waring MA. 1995. The variable metabolic response to dietary isoflavones in humans. Proceedings of the Society of Experimental Biology and Medicine 208, 40-43.

Kong   J-M, Chia L-S, Goh N-K, Chia T-F, Brouillard R. 2003. Analysis and biological activities of anthocyanins. Phytochemistry 64, 923-933.http://dx.doi.org/10.1016/s00319422(03)00438-2

Kopcewicz J. 1971. Estrogen in developing bean (Phaseolus vulgaris) plants Phytochemestry 10, 1423-1427

Kurzer MS, Xu X. 1997. Dietary phytoestrogens, Annual Revue of Nutrition 17, 353-381. http://dx.doi.org/10.1146/annurev.nutr.17.1.353

Lee YB, Lee HJ, Kim KS, Lee JY, Nam SY, Cheon SH, Sohns HS. 2004. Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats. Biosciences Biotechnology and Biochemistry 68(5), 1040-1045. http://dx.doi.org/10.1271/bbb.68.1040

Mazur W. 1998. Phytoestrogen content in foods, Baillieres Clinical Endocrinology Metabolism 12, 729-742.

Mizutani K, Ikeda K, Kawai Y, Yamori Y. 1998. Resveratrol stimulates the proliferation and differentiation of osteoblastics MC3T3-E1 cells. Biochemical and Biophysical Resources Communication 253, 859-863. http://dx.doi.org/10.1006/bbrc.1998.9870

Morabito N, Crisafulli A, Vergara C, Gaudio A, Lasco A, Frisina N, D’anna R, Corrado F, Pizzoleo MA, Cincotta, Altavilla D, Lentile R, Squadrito F. 2002. Effects of genistein and hormone-replacement therapy on bone loss in early postmenopausal women: a randomized double-blind placebo-controlled study. Journal Bone Mineral Resource 17, 1904–1912. http://dx.doi.org/10.1359/jbmr.2002.17.10.1904

Oh K-O, Kim S-W, Kim J-Y, Ko S-Y, Kim H-M, Baek J-H, Ryoo H-M, Kim J-K. 2003. Effect of Rehmannia glutinosa Libosch extracts on bone metabolism. Clinica chimica Acta 334, 185-195. http://dx.doi.org/10.1016/s0009-8981(03)00238-9

Ogata H, Hayashi M, Tsuda H, Suzuki N, Maeno M, Sugawara A, Ogiso B. 2012. Effects of calcium phosphate cement on ineralized nodule formation compared with endodontic cements. Dental materials Journal 31(1), 92-97. http://dx.doi.org/10.4012/dmj.2011-151

Ososki AL, Kennely EJ. 2003. Phytoestrogens: a review of the present state of research. Phytotherapy Research 17, 845-869. http://dx.doi.org/10.1002/ptr.1364

Om AS, Shim JY. 2007. Effects of daidzein, a soy isoflavone, on bone metabolism in cd-treated ovariectomized rats. Acta Biochimica Polonica 54(3), 641-647.

Pari L, Venkateswaran S. 2003. Effect of an aqueous extract of Phaseolus vulgaris on the properties of tail tendon collagen of rats with streptozotocin-induced diabetes. Brazilian Journal of Medical and Biological Research 36, 861-870. http://dx.doi.org/10.1590/s0100-879×2003000700006

Rassi CM, Lieberherr M, Chaumaz G, Pointillart A, Cournot G. 2002. Down-regulation of osteoclast differentiation by daidzein via caspase 3. Journal of Bone Mineral Resources 17(4), 630-638. http://dx.doi.org/10.1359/jbmr.2002.17.4.630

Reynoso-Camacho R, Ramos-Gomez M, Loarca-Pina G. 2006. Bioactive components in common beans (Phaseolus vulgaris L.). Advances in Agricultural and food Biotechnology, 217-236.

Shirke SS, Jadhav SR, Jaqtap AG. 2009. Oteoprotective effects of Phaseolus vulgaris L. in ovariectomized induced osteopenia in rats. Menopause 16(3), 589-596. http://dx.doi.org/10.1097/gme.0b013e31818e64c4

Sugimoto E, Yamaguchi M. 2000. Anabolic effect of genistein in osteoblastic MC3T3-E1 cells. International Journal of Molecular Medicine 5(5), 515-520.

Wiseman H. 1999. The bioavailability of non-nutrient plant factors: dietary flavonoids and phyto-estrogens, Proceedings of the Society of Experimental Biology and Medicine 58, 139-146. http://dx.doi.org/10-1079/pns19990019

Xu X, Harris KS, Wang H-J, Murphy PA, Hendrich S. 1995. Biovailability of soybean isoflavones depends upon gut microflora in women. Journal of Nutrition 125, 2307-2315.

Yamagushi M, Weitzmann MN. 2009. The estrogen  17β-estradiol  and  phytoestrogen  genistein mediate  differential  effects       on  osteoblastic  NF-κB activity. International Journal of Molecular Medicine 23(2), 297-301. http://dx.doi.org/10.3892/ijmm_00000131

Ye S-F, Saga I, Ichimura K, Nagai T, Shinoda M, Matsuzaki S. 2003. Coumestrol as well as isoflavones in soybean extract prevent bone resorption in ovariectomized rats. Endocrine regulations 37(3), 145-152.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background