Studies on phytochemical screening, total phenolic content and antiradical activity of three extracts of Emilia sagittata DC. (Asteraceae)

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Research Paper 01/04/2013
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Studies on phytochemical screening, total phenolic content and antiradical activity of three extracts of Emilia sagittata DC. (Asteraceae)

Joseph Privat Ondo, Louis-Clément Obame, Gontran Nsi Akoue, Jean-Pierre Medzo Engono, Soulemane Parkouda, Edouard Nsi Emvo, Jacques Lebibi
Int. J. Biosci. 3(4), 50-57, April 2013.
Copyright Statement: Copyright 2013; The Author(s).
License: CC BY-NC 4.0

Abstract

The present study determined the phytochemicals, antiradical activity, total phenolic and proanthocyanidin contents of three extracts of Emilia sagittata which was an herbaceous annual currently used to treat various diseases in gabonese pharmacopeia. The powdered plant samples were analyzed for their phytochemical screening using standard laboratory methods. The total phenols were measured using the Folin-Ciocalteu method. Proanthocyanidins content were determined by the method of HCl-butanol hydrolysis. Antioxidant activities were evaluated with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The best average contents of total phenols and proanthocyanidins were revealed among 423.80 mg GAE/10 mg extract and 57.11 mg APE/10 mg extract respectively, and an average of antioxidant capacity was found in 170.24 μg/ml of extract. Somewhat the proanthocyanidins in extract represent an average of 13.69 % for the total phenolic content. Also an average of 93% antioxidant capacity of extracts was due to the contribution of proanthocyanidins compounds thus are the dominant antioxidants in these extracts. The presence variability of bioactive extract compounds in the case of Emilia sagittata consolidated the fact of the traditional used of that plant in certain treatment diseases.

Abdoul-latif FM, Bayili RG, Obame LC, Bassolé IHN, Dicko MH. 2012. Comparison of phenolic compounds and antioxidant capacities of traditional sorghum beers with other alcoholic beverages. African Journal of Biotechnology 11(81), 14671-14678. http:/dx.doi.org/10.5897/AJB12.2153

Azuma Y, Nakawo KM, Jiankany H, Watanabe O .1995. Metting induced relocation of ions in glacier and in a seasonal snow-pack. IAHS Predictions in Ungauged Basins 223, 287-287.

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-2000. http:/dx.doi.org/10.1038/1811199a0

Bruneton J. 2009. Pharmacognosie, Phytochimie, plantes médicinales. 4ème édition, Tec & Doc

Burkill, HM. 1985. The useful plants of west tropical Africa, Vol 1.

Cao GH, Prior RL. 1999. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clinical Chemistry 44, 1309-1315.

Cherian S, Augustin KT. 1995. Insulin sparing action of leucopelargonidin derivative isolated from Ficus bengalesis Linn. Indian Journal of Experimental Biology 33, 608-611.

Culei I. 1982. Methodology for the analysis of vegetable drugs. Practical manual on the industrial utilization of medicinal and aromatic plants. Center Building, Romania, 67-81.

Harbone JB. 1984. Phytochemical methods. 2nd Edn. Champion and Hall Publishers, London, 84-196.

Njintang YN, Tatsadjieu Ngoune L, Ngakou A, Danra D, Tchuenguem-Fohouo FN. 2012. Antiradical activity and polyphenol content of ethanolic extracts of Propolis. International Journal of Biosciences 2(4), 56-63.

Nostro A, Germano MP, D’Angelo V, Cannatelli MA. 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology 30(5), 379-384. http:/dx.doi.org/10.1046/j.1472-765x.2000.00731.x

Odebiyi OO, Sofowora EA. 1979. Phytochemical screening of Nigerian Medicinal plants. 2nd OAU/STRC Inter-African Symposium on traditional Phamacopoeia and African Medicinal, p. 216-220.

Olajire AA, Azeez L. 2011. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African Journal of Food Science and Technology 2(2), 22-29.

Oloyed OI. 2005. Chemical profile of unripe pulp of Carica pagaya. Pak. Journal of Nutrition 4, 379-381.

Parekh J, Karathia N, Chanda S. 2006.Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegata L. bark. African Journal of Biomedical Research 9(1), 53-56.

Park YS, Jeon MH, Hwang HJ, Park MR, Lee SH, Kim SG, Kim M. 2011. Antioxidant activity and analysis of proanthocyanidins from pine (Pinus densiflora) needles. Nutrition Research and Practice 5(4), 281-287. doi. http:/dx.doi.org/10.4162/nrp.2011.5.4.281

Prigent S. 2005. Interactions of phenolics compounds with globular proteins and their effects on food related functional properties. PhD Thesis, Wageningen University, the Netherlands. p. 132.

Raponda-Walker A et Sillans R. 1961. Les plantes utiles du Gabon. Paul Lechevalier, Paris, France. p. 128.

Santos-Buelga C, Scalbert A. 2000. Proanthocyanidins and tannin-like compounds– nature, occurrence, dietary intake and effects on nutrition and health. Journal of the Science of Food and Agriculture 80(7), 1094-1117.

Singleton VL, Orthofer R, Lamuela-Raventos RM.  1999.  Analysis  of  total  phenols  and  other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299, 152-179. http://dx.doi.org/10.1016/S0076-6879(99)99017-1

Sofowora A. 1993. Medicinal plants and traditional medicine in Africa. 2nd Edn. Spectrum Books limited, Ibadan, Nigeria, 1-153.

Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. 2007. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry 104, 1372-1378. http://dx.doi.org/10.1016/j.foodchem.2007.01.064

Trease GE, Evans WC.  2002.  Pharmacognosy. 15th Edn. Saunders, 214-393.

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