Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

Paper Details

Research Paper 03/04/2024
Views (1229)
current_issue_feature_image
publication_file

Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

R. Kavitha, S. Sahaya Sathish, S. Bavya, G. Jahirhussain, Amzad Basha Kolar, R. Kavitha
Int. J. Biosci. 24(4), 83-90, April 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Lygodium microphyllum, a perennial climbing fern from the Lygodiaceae family, is known for its various pharmacological benefits, mainly playing a role in enhanced digestion, increased energy levels, and boosted immunity due to its rich phytoconstituents. In phytochemical analysis, the UV-Vis analysis of in vitro cultured Lygodium microphyllum in ethanolic extract revealed the peaks at 238, 319, 398, 500, and 665 nm, indicating absorptions of 3.850, 4.000, 2.346, 0.176, and 0.411, respectively. These peaks signify the presence of carbonyl and nitroso groups within organic chromophores in the plant extract. Furthermore, the FTIR revealed the presence of carbonyls, alkynes, phenols, dialkyl groups, aromatic ethers, and aliphatic fluoro compounds. Analysis of GC-MS identified a total of 30 compounds. Regarding antibacterial activity, chloroform extract of in vitro cultured Lygodium microphyllum exhibited the highest inhibition rate recorded against the Bacillus cereus (19.23 ±2.12mm). Next to this the ethyl acetate extract of the fern shows significant inhibition against Klebsiella pneumoniae (19.00 ±1.48mm). However, in terms of antifungal activity, all tested fungal strains displayed minimal zone of inhibition.

Dudani SN, Mahesh MK, Chandran MS, Ramachandra TV. 2014. Pteridophyte diversity in wet evergreen forests of Sakleshpur in Central Western Ghats. Indian Journal of Plant Sciences 3(1), 28-29.

Parihar P, Parihar L. 2006. Some pteridophytes of medicinal importance from Rajasthan.

Raven Ph. 1999. World’s biodiversity becoming extinct at levels rivaling earth’s past “mass extinctions”. Botanic Garden Conservation News 3, 31–32.

Manickam VS.  1995. Rare and endangered ferns of the Western Ghats of South India. Fern Gazette 15, 1–10.

Maxted N, Ford-lloyd B, Hawkes JG. (Eds.). 1997. Plant Genetic Conservation: The In situ Approach. Chapman & Hall. London.

Rathee D, Rathee P, Rathee S, Rathee D. 2016 Phytochemical screening and antimicrobial activity of Picrorrhiza kurroa, a traditional Indian plant used to treat chronic diarrhea. Arabian Journal of Chemistry, 9, S1307–13.

Pradeep P, Leena P, Achaleshwar B. 2010. In vitro antibacterial activity of fronds (leaves) of some important pteridophytes. Journal of Microbiology and Antimicrobials 2(2), 19-22.

Kora AJ, Rastogi L. 2013. Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria. Bioinorganic chemistry and applications, 1–7.

Parveen A, Roy AS, Rao S. 2012. Biosynthesis and characterization of silver nanoparticles from Cassia auriculata leaf extract and in vitro evaluation of antimicrobial activity. International journal of applied biology and pharmaceutical technology 3(2), 222 – 228.

Jain PK, Soni A, Jain P, Bhawsar J. 2016. Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. Journal of Chemical and Pharmaceutical Research 8(2), 1-6.

Jeeshna MV. 2017. Antimicrobial activities and phytochemical investigation of fern, Lygodium flexuosum (Linn) SW. Kongunadu Research Journal 4(1), 87-91.

Related Articles

Influence of climatic factors on the spatial and temporal distribution of mealybugs, vectors of swollen shoot disease of cocoa tree in Koda, South-West Côte d’Ivoire

Akoua Miézan Claudine N’guettia, Zokou Franck Oro, Yédé Jean Aliko, San-Whouly Mauricette Ouali N’goran, Int. J. Biosci. 28(3), 115-124, March 2026.

Surveillance and detection of the occurrence of African swine fever in abattoirs in the different municipalities of the second district of Cagayan, Philippines

Maricel F. Campanano, Dennis M. Oyardo, Mary Ann M. Santos*, Int. J. Biosci. 28(3), 106-114, March 2026.

Spawn preparation and cultivation of Volvariella volvacea (Bull. ex Fr.) Singer on paddy straw substrate

A. Anees Fathima*, J. Jayasree, Int. J. Biosci. 28(3), 97-105, March 2026.

Effects of dairy cattle rotational grazing on soil properties in the grassland area of CSU Piat, Cagayan

Andrea Flores Dawan*, Nonito Baliuag Pattugalan, Juan Sales Daquioag II, Int. J. Biosci. 28(3), 90-96, March 2026.

Diversity of melliferous plants in a forest ecosystem in the Sudanian zone: The case of the Badenou classified forest in northern Côte d’Ivoire

Dofoungo Koné*, Pagadjovongo Adama Silué, Fofana Séguéna, Bruno Marcel Iritié, Doudjo Noufou Ouattara, Wandan Eboua Narcisse, Int. J. Biosci. 28(3), 80-89, March 2026.

Recent findings on the anticancer potential of coumarin hybrid derivatives

Mohd Akil, Chandra Shekhar Yadav, Atul Krishna, Vijay Kumar Verma, Iqbal Azad*, Tridev Katiyar, Amar Chandra Sharma, Mirza Masroor Ali Beg, Int. J. Biosci. 28(3), 52-79, March 2026.

The main diseases of cucumber (Cucumis sativus L.) grown in the Republic of Azerbaijan and the species composition of pathogens of these diseases

K. F. Bakhshaliyeva*, A. Kh. Rajabli, A. G. Eyvazov, G. A. Gasimova, P. Z. Muradov, Int. J. Biosci. 28(3), 45-51, March 2026.