Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

Paper Details

Research Paper 03/04/2024
Views (1541)
current_issue_feature_image
publication_file

Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

R. Kavitha, S. Sahaya Sathish, S. Bavya, G. Jahirhussain, Amzad Basha Kolar, R. Kavitha
Int. J. Biosci. 24(4), 83-90, April 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Lygodium microphyllum, a perennial climbing fern from the Lygodiaceae family, is known for its various pharmacological benefits, mainly playing a role in enhanced digestion, increased energy levels, and boosted immunity due to its rich phytoconstituents. In phytochemical analysis, the UV-Vis analysis of in vitro cultured Lygodium microphyllum in ethanolic extract revealed the peaks at 238, 319, 398, 500, and 665 nm, indicating absorptions of 3.850, 4.000, 2.346, 0.176, and 0.411, respectively. These peaks signify the presence of carbonyl and nitroso groups within organic chromophores in the plant extract. Furthermore, the FTIR revealed the presence of carbonyls, alkynes, phenols, dialkyl groups, aromatic ethers, and aliphatic fluoro compounds. Analysis of GC-MS identified a total of 30 compounds. Regarding antibacterial activity, chloroform extract of in vitro cultured Lygodium microphyllum exhibited the highest inhibition rate recorded against the Bacillus cereus (19.23 ±2.12mm). Next to this the ethyl acetate extract of the fern shows significant inhibition against Klebsiella pneumoniae (19.00 ±1.48mm). However, in terms of antifungal activity, all tested fungal strains displayed minimal zone of inhibition.

Dudani SN, Mahesh MK, Chandran MS, Ramachandra TV. 2014. Pteridophyte diversity in wet evergreen forests of Sakleshpur in Central Western Ghats. Indian Journal of Plant Sciences 3(1), 28-29.

Parihar P, Parihar L. 2006. Some pteridophytes of medicinal importance from Rajasthan.

Raven Ph. 1999. World’s biodiversity becoming extinct at levels rivaling earth’s past “mass extinctions”. Botanic Garden Conservation News 3, 31–32.

Manickam VS.  1995. Rare and endangered ferns of the Western Ghats of South India. Fern Gazette 15, 1–10.

Maxted N, Ford-lloyd B, Hawkes JG. (Eds.). 1997. Plant Genetic Conservation: The In situ Approach. Chapman & Hall. London.

Rathee D, Rathee P, Rathee S, Rathee D. 2016 Phytochemical screening and antimicrobial activity of Picrorrhiza kurroa, a traditional Indian plant used to treat chronic diarrhea. Arabian Journal of Chemistry, 9, S1307–13.

Pradeep P, Leena P, Achaleshwar B. 2010. In vitro antibacterial activity of fronds (leaves) of some important pteridophytes. Journal of Microbiology and Antimicrobials 2(2), 19-22.

Kora AJ, Rastogi L. 2013. Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria. Bioinorganic chemistry and applications, 1–7.

Parveen A, Roy AS, Rao S. 2012. Biosynthesis and characterization of silver nanoparticles from Cassia auriculata leaf extract and in vitro evaluation of antimicrobial activity. International journal of applied biology and pharmaceutical technology 3(2), 222 – 228.

Jain PK, Soni A, Jain P, Bhawsar J. 2016. Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. Journal of Chemical and Pharmaceutical Research 8(2), 1-6.

Jeeshna MV. 2017. Antimicrobial activities and phytochemical investigation of fern, Lygodium flexuosum (Linn) SW. Kongunadu Research Journal 4(1), 87-91.

Related Articles

Stabilization of cashew apple juice with chitosan: clarification and microbiological quality

Kokora Aya Philomène*, Coulibaly Adama, Cissé Mohamed, Coulibaly Bintou, Konan N’guessan Ysidor, Godi Henri Marius Biego, Int. J. Biosci. 29(2), 76-88, August 2026.

Implementation and evaluation of limited face to face classes: basis for adoption of health and safety manual

Christian Jude B. Calunsag*, Int. J. Biosci. 29(2), 65-75, August 2026.

Baseline characterization of farming systems in the province of Bassitenga in Burkina Faso

Boly Ismaël Ardho*, Traoré Mamadou, Hema Alain Sabiriba, Sory Lallé Daouda, Int. J. Biosci. 29(2), 54-64, August 2026.

Zootechnical performance and economic profitability of sasso broiler chickens reared under a semi-automatic production system in Burkina Faso

Bernadette Yougbaré*, Arnaud Stéphane Rayangnéwêndé Tapsoba, Fatogoman Téophile Sanou, Clément Kaboré, Amadou Traoré, Int. J. Biosci. 29(2), 35-44, August 2026.

Factors influencing the use of Mucuna pruriens as food in Benin, West Africa

Yves Boladé Djimba, Janvier Mêlégnonfan Kindossi*, Folachodé Ulrich Gildas Akogou, Ephraïm Olouwafemi Biao, Jacques Agangni, Kokou Mensah Adjangba, Ogouyôm Herbert Iko Afé, Franck Hongbété, Rodrigue V. Cao Diogo, Int. J. Biosci. 29(2), 19-34, August 2026.

Contribution to the inventorier and establishment of a collection of agricultural entomofauna from the Niayes area of Dakar

Mamecor Faye*, Amy Collé Guèye, Coumba Gning, Lamine Konaté, Mbacké Sembène, Int. J. Biosci. 29(2), 10-18, August 2026.

Assessment of the trophic ecology and variation in the natural diet of an anuran amphibian species (Afrixalus dorsalis) in the Wetlands of the Ehotilé Islands (Ivory Coast)

Beh Romaric Konate*, Amani Reine Élisabeth Kouadio, Louis César Agou, Enoutchy Fabrice Bouah, N'Guessan Ludovic Yao, Etilé Raphaël N’doua, Int. J. Biosci. 29(2), 1-9, August 2026.