Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

Paper Details

Research Paper 03/04/2024
Views (1158)
current_issue_feature_image
publication_file

Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

R. Kavitha, S. Sahaya Sathish, S. Bavya, G. Jahirhussain, Amzad Basha Kolar, R. Kavitha
Int. J. Biosci. 24(4), 83-90, April 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Lygodium microphyllum, a perennial climbing fern from the Lygodiaceae family, is known for its various pharmacological benefits, mainly playing a role in enhanced digestion, increased energy levels, and boosted immunity due to its rich phytoconstituents. In phytochemical analysis, the UV-Vis analysis of in vitro cultured Lygodium microphyllum in ethanolic extract revealed the peaks at 238, 319, 398, 500, and 665 nm, indicating absorptions of 3.850, 4.000, 2.346, 0.176, and 0.411, respectively. These peaks signify the presence of carbonyl and nitroso groups within organic chromophores in the plant extract. Furthermore, the FTIR revealed the presence of carbonyls, alkynes, phenols, dialkyl groups, aromatic ethers, and aliphatic fluoro compounds. Analysis of GC-MS identified a total of 30 compounds. Regarding antibacterial activity, chloroform extract of in vitro cultured Lygodium microphyllum exhibited the highest inhibition rate recorded against the Bacillus cereus (19.23 ±2.12mm). Next to this the ethyl acetate extract of the fern shows significant inhibition against Klebsiella pneumoniae (19.00 ±1.48mm). However, in terms of antifungal activity, all tested fungal strains displayed minimal zone of inhibition.

Dudani SN, Mahesh MK, Chandran MS, Ramachandra TV. 2014. Pteridophyte diversity in wet evergreen forests of Sakleshpur in Central Western Ghats. Indian Journal of Plant Sciences 3(1), 28-29.

Parihar P, Parihar L. 2006. Some pteridophytes of medicinal importance from Rajasthan.

Raven Ph. 1999. World’s biodiversity becoming extinct at levels rivaling earth’s past “mass extinctions”. Botanic Garden Conservation News 3, 31–32.

Manickam VS.  1995. Rare and endangered ferns of the Western Ghats of South India. Fern Gazette 15, 1–10.

Maxted N, Ford-lloyd B, Hawkes JG. (Eds.). 1997. Plant Genetic Conservation: The In situ Approach. Chapman & Hall. London.

Rathee D, Rathee P, Rathee S, Rathee D. 2016 Phytochemical screening and antimicrobial activity of Picrorrhiza kurroa, a traditional Indian plant used to treat chronic diarrhea. Arabian Journal of Chemistry, 9, S1307–13.

Pradeep P, Leena P, Achaleshwar B. 2010. In vitro antibacterial activity of fronds (leaves) of some important pteridophytes. Journal of Microbiology and Antimicrobials 2(2), 19-22.

Kora AJ, Rastogi L. 2013. Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria. Bioinorganic chemistry and applications, 1–7.

Parveen A, Roy AS, Rao S. 2012. Biosynthesis and characterization of silver nanoparticles from Cassia auriculata leaf extract and in vitro evaluation of antimicrobial activity. International journal of applied biology and pharmaceutical technology 3(2), 222 – 228.

Jain PK, Soni A, Jain P, Bhawsar J. 2016. Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. Journal of Chemical and Pharmaceutical Research 8(2), 1-6.

Jeeshna MV. 2017. Antimicrobial activities and phytochemical investigation of fern, Lygodium flexuosum (Linn) SW. Kongunadu Research Journal 4(1), 87-91.

Related Articles

Sensory evaluation of horn snail (Telescopium telescopium) patty

Ma. Isabel P. Lanzaderas, Gilbert P. Panimdim, Proceso C. Valleser Jr.*, Int. J. Biosci. 28(2), 7-16, February 2026.

Two years evolution of deltamethrin, malathion and pirimiphos-methyl resistance in Aedes aegypti from urban in peri urban sites of Ouagadougou, Burkina Faso

Hyacinthe K. Toe*, Moussa W. Guelbeogo, Soumananaba Zongo, Aboubacar Sombie, Athanase Badolo, Int. J. Biosci. 28(2), 1-6, February 2026.

Physicochemical characterization of annatto seeds (Bixa orellana) sold in Ouagadougou and their oils extracted using chemical processes

Mah Alima Esther Traoré*, Adama Lodoun, Pingdwindé Marie Judith Samadoulougou-Kafando, Nestor Beker Dembélé, Kiswendsida Sandrine Léticia Dayamba, Charles Parkouda, Int. J. Biosci. 28(1), 169-178, January 2026.

Inventory of african yam bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms) diversity in some Yoruba areas of Benin

Orobiyi Azize*, Faton Manhognon Oscar Euloge, Zongo Élisabeth Aboubié, Sossou Kpèdé Nicodème, Houngbo Marcel, Dossou Pierre Fourier, Ogoudjobi Ladékpo Sylvain, Balogoun Ibouraïman, Dansi Alexandre, Lokoyêyinou Laura Estelle, Int. J. Biosci. 28(1), 161-168, January 2026.

A severe case of human hepatic fascioliasis mimicking an oncological disease in Azerbaijan

Aygun A. Azizova*, Int. J. Biosci. 28(1), 155-160, January 2026.

Combined effect of irrigation frequency and leaf harvesting intensity on soil water content and productivity of baobab (Adansonia digitata) seedlings in vegetable production

Sissou Zakari, Imorou F. Ouorou Barrè, Mouiz W. I. A. Yessoufou*, Colombe E. A. E. Elegbe, Amamath S. Boukari, P. B. Irénikatché Akponikpè, Int. J. Biosci. 28(1), 143-154, January 2026.

Develop sustainable coffee-based farming model using cash crops production

Maribel L. Fernandez, Roje Marie C. Rosqueta*, Diosa G. Alasaas, Boyet C. Pattung, Jaylord Dalapo, Janette Empleo, Int. J. Biosci. 28(1), 134-142, January 2026.

Animal anthrax in northern Tanzania (2015-2025): Epidemiological trends and frontline response capacity

Yohana Michael Kiwone*, Beatus Lyimo, Rowenya Mushi, Joram Buza, Int. J. Biosci. 28(1), 123-133, January 2026.