Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

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Research Paper 03/04/2024
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Studies on the phytochemical properties and antimicrobial activities of in vitro cultured Lygodium microphyllum (CAV.) R. BR.

R. Kavitha, S. Sahaya Sathish, S. Bavya, G. Jahirhussain, Amzad Basha Kolar, R. Kavitha
Int. J. Biosci.24( 4), 83-90, April 2024.
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Abstract

Lygodium microphyllum, a perennial climbing fern from the Lygodiaceae family, is known for its various pharmacological benefits, mainly playing a role in enhanced digestion, increased energy levels, and boosted immunity due to its rich phytoconstituents. In phytochemical analysis, the UV-Vis analysis of in vitro cultured Lygodium microphyllum in ethanolic extract revealed the peaks at 238, 319, 398, 500, and 665 nm, indicating absorptions of 3.850, 4.000, 2.346, 0.176, and 0.411, respectively. These peaks signify the presence of carbonyl and nitroso groups within organic chromophores in the plant extract. Furthermore, the FTIR revealed the presence of carbonyls, alkynes, phenols, dialkyl groups, aromatic ethers, and aliphatic fluoro compounds. Analysis of GC-MS identified a total of 30 compounds. Regarding antibacterial activity, chloroform extract of in vitro cultured Lygodium microphyllum exhibited the highest inhibition rate recorded against the Bacillus cereus (19.23 ±2.12mm). Next to this the ethyl acetate extract of the fern shows significant inhibition against Klebsiella pneumoniae (19.00 ±1.48mm). However, in terms of antifungal activity, all tested fungal strains displayed minimal zone of inhibition.

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