Studies on toxicological responses of the herbicide paraquat dichloride on Monopterus cuchia (Hamilton)

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Studies on toxicological responses of the herbicide paraquat dichloride on Monopterus cuchia (Hamilton)

Manas Das, Khusnuma Ahmed, AnuradhaKalita, Leena Das, Pritimoni Das
Int. J. Biosci.12( 5), 116-132, May 2018.
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Paraquat dichloride is considered to be highly toxic in nature and is widely used in India. The present work aimed to investigate the toxicity induced by Paraquat on Monopterus cuchia exposed to the sub-lethal concentrations of 2 mg/L and 4 mg/L respectively. The study was conducted with emphasis on the haematological parameters, antioxidant enzyme assays and histopathology. The total erythrocyte count, hemoglobin concentration and MCH showed significant reduction, while the total leucocyte count significantly increased by 1.5 fold and 1.83 fold in low and high dose respectively compared to control. The study showed reduction in the activities of superoxide dismutase in liver, kidney and intestine in both the treated groups. However, there was a significant reduction in the activity by 2.98 fold in liver, 2.45 fold in kidney and 1.73 fold in intestine respectively in the high dose compared to control. Significant reduction in the activities of catalase and reduced glutathione in comparison to the control was observed. The catalase activity was reduced by 2.37 fold in the liver, 2.67 fold in the kidney and 3.54 fold in the intestine in the group treated with high dose. However, glutathione-S-transferase activity was found to increase in liver, kidney and intestine with significant increase by 2.28 fold in kidney as compared to control when treated with high dose. Histopathology of liver, kidney and intestine exhibited alterations in structures in treated groups. Thus, the changes observed during the study period establish that Paraquat is highly toxic and thereby its use should be restricted.


Ahmad I, Hamid, Fatima M, Chand HS, Jain SK, Athar M, Raisudin S. 2000. Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. Biochimicaet Biophysica Acta 1523, 37-48.

Anithakumari S, Ram Kumar NS. 1995. Effect of water pollution on the histology of fish Channa striatus in Hissainsagar Lake, Hyderabad. Indian Environment and Ecology 13, 932-934.

Arivu I, Muthulingam M, Jiyavudeen M. 2016. Toxicity of paraquat on freshwater fingerlings of Labeorohita (Hamilton). International Journal of Scientific & Engineering Research 7(10), 2229-5518.

Banaee M, Sureda A, Mirvagefei AR, Ahmadi K. 2013a. Biochemical and histological changes in the liver tissue of Rainbow trout (Oncorhynchus mykiss) exposed to sub-lethal concentrations of diazinon. Fish Physiology and Biochemistry (in press).

Banaee M, Sureda A, Mirvagefei AR, Ahmadi K. 2013b. Histopathological alterations induced by diazinon in rainbow trout, (Oncorhynchus mykiss). International Journal of Environmental Research (in press).

Barreto-Medeiros JM, Feitoza EG, Magalhães K, Da Silva RR, Manhães-de-Castro FM, Manhães-de-Castro R, De-Castro CMMB. 2005. The expression of an intraspecific aggressive reaction in the face of a stressor agent alters the immune response in rats. Brazilian Journal of Biology 65, 203–209.

Beutler E. 1975. Red Cell Metabolism: A Manual of Biochemical Methods. Grune & Straton, New York.

Beutler E, Duron O, Kelly BM. 1963. Improved method for the determination of blood glutathione. Journal of Laboratory Clinical Medicine 61, 882–888.

Bhargava S, Dixit RS, Rawat M.1999. BHC and Malathion induced changes in TLC and DLC of Channa striatus. Proceedings of the Academy of  Environmental Biology 8, 91-94.

Borvinskaya EV, Smirnov LP, Nemova NN. 2013. An alpha class glutathione S-transferase from pike liver. Russian Journal of  Bioorganic Chemistry 39, 498–503.

Carlos MP, António JM, Vitor MC, Madeira. 1995. Mitochondrial Bioenergetics is affected by the herbicide Paraquat. Biochimicaet Biophysica Acta 1229, 187-192.

Clifton G, Kaplowitz N. 1978.Effect of dietary phenobarbital, 3, 4-benzo (cu) pyrene and 3-methylcholanthrene on hepatic, intestinal and renal glutathione-S-transferase activities in the rat. Biochemical Pharmacology 27, 1287-1288.

Das M, Bhattacharjee S, Nath N. 2015. Sub-lethal toxic effect of cadmium heavy metal in situ exposure in fresh water mud eel Monopterus cuchia. Tropical Zoology 5, 75-84.

Dautremepuits C, Marcogliese DJ, Gendron AD, Dautremepuits MF. 2009. Gill and head kidney antioxidant processes and innate immune system responses of yellow perch (Perca flavescens) exposed to different contaminants in the St. Lawrence River Canada. Science of the Total Environment 407, 1055–1064.

Figueiredo-Fernandes A, Fontaı´nhas-Fernandes A, Peixoto F, Rocha E, Reis-Henriques MA. 2006. Effects of gender and temperature on oxidative stress enzymes in Nile tilapia Oreochromis niloticus exposed to Paraquat. Pesticide Biochemistry and Physiology 85, 97–103.

Flohé L, Otting F. 1984. Superoxide dismutase assays. Methos in Enzymology 105, 93–104.

Goel KA, Garg V. 1980. Histopathological changes produced in liver and kidney of Channa punctatus after chronic exposure to 2, 3, 4-triaminobenzene. Bulletin of Environmental Contamination and Toxicology 25, 330-334.

Gwathway CO, Craig Jr C. 2007. Defoliants for cotton.Encyclopedia of pest management. Boca Raton: CRC Press 135-137.

Hanson-Painton O, Griffin MJ, Tang J.1983. Involvement of a cytosolic carrier protein in the microsomal metabolism of benzo [a] pyrene in rat liver. Cancer Research 43, 4198-4206.

Hjeltnesl B, Samuelsen O, Martin Svarda A. 1992. Changes in plasma and liver glutathione levels in Atlantic salmon Salmosalar suffering from infectious salmon anemia (ISA). Diseases of  Aquatic Organisms 14, 31- 33.

Jain NC. 1993. Essentials of Veterinary Hematology, Lea &Febiger, Philadelphia 32 p.

Jakoby WB.1978.The glutathione-S-transferases: a group of multi-functional detoxification proteins. Advances in Enzymology 46, 383-414.

Jha BS. 1999. Impact of chronic exposure of the household detergents, surf and key, on tissue biotechnology of the fresh water fish Clarias batrachus (Linn.). Indian Journal of Environment and Ecoplanning2, 281-284.

Kathowaska K, Szubartowska E, Ecezanowaska E.1985. Pheripheral blood the Japanese quail (Coturnix coturnix japonica) in acode poisoning by different insecticides, Comparative Biochemistry and Physiology 81c(1), 209-212.

Khillare YK. 1985. Toxicological effects of pesticides on freshwater fish, Barbus stigma (Ham.). Ph.D. Thesis, Marathwada University, Aurangabad.

Koundinya PR, Ramamurthy R. 1979. Effect of organophosphate sumithion (Fenthion) on some aspects of carbohydrate metabolism in a fresh water fishes Sarotherodon mossambicus and Tilapia mossambicus (Peters). Experientia 15, 1632-1633.

Kumar D. 2015.Conditions of Paraquat Use in India. Joint study by PAN India, IUF and Berne Declaration.

Ladipo MK, Doherty VF, Oyebadejo SA. 2011. Acute Toxicity, Behavioural Changes and Histopathological Effect of Paraquat Dichloride on Tissues of Catfish (Clarias gariepinus). International Journal of Biology 2, 67-74.

Lai ASB, Anitakumari S, Sinha RN.1986. Biochemical and haematological changes following malathion treatment in the freshwater catfish Heteropneustes fossilis (Bloch). Environmental Pollution 42(A), 151-156.

Lima HM. 2004. Oxygen in biology and biochemistry. In: Storey, K.B., (Ed.), Functional Metabolism: Regulation and Adaptation 12, 319–368.

Ma J, Li Y, Niu D, Li Y, Li X. 2014. Immunological effects of paraquat on common carp, Cyprinus carpio L. Fish and Shellfish Immunology 37, 166-72.

Ma J, Li X, Li Y, Li Y, Niu D. 2014. Toxic Effects of Paraquat on Cytokine    Expression in Common Carp, Cyprinuscarpio L. Journal of Biochemical and Molecular Toxicology 28, 11, 501-509.

McLeay DJ. 1973. Effect of 12 and 25 days exposure to kraft pulp mill effluent on the blood and tissue of juvenile Coho salmon Oncorhynchus kisutch. Journal of the Fisheries Research Board Canada 30, 395-400.

Meister A, Anderson ME.1983. Glutathione. Annual Review of Biochemistry 52, 7 11-760.

Modesto KA, Martinez CBR.2010. Roundup causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus. Chemosphere 78, 294–299.

Moitra A, Lai R.1989. Effect on sublethal doses of malathion and BHC on the intestine of the fish, Puntius sarana. Environment and Ecology 7, 412-414.

Monteiro DA, Almeida JA, Rantin FT, Kalinin AL. 2006. Oxidative stress biomarkers in the freshwater characid fish, Bryconcephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology 143C, 141–149.

Natarajan GM. 1981. Changes in the blood parameters in air breathing fish, Channa straitus following lethal (LC50/48h) exposed to metasystox (Dimethoate). Current Science 50, 40-41.

Nemoto N, Gelboin H. 1975. Assay and properties of glutathlone-S-benzo (a) pyrene-4, 5-oxide transferase. Archives of Biochemistry and Biophysics 170, 739-742.

Nithyanandam TG, Maruthanayagam C, Viswanathan P. 2007. Effects of sublethal level of a pesticide, monocrotophos, on haematology of Cyprinus carpio during the exposure and recovery periods. Natrure Environment & Pollution Technology 6, 615-621.

OECD.1992. Organization for Economic Co-operation and Development (OECD).Guideline 203. OECD Guideline for Testing of Chemicals: Fish, Acute Toxicity.

Otto DM, Moon TW. 1995. 3,30,4,40,-Tetrachlorobiphenyl effects on antioxidant enzymes and glutathione status in different tissues of rainbow trout. Pharmacology & Toxicology 77, 281–287.

Pacheco M, Santos M A. 2002. Biotransformation, genotoxic and histopathological effects of environmental contaminants in European eel, Anguilla anguilla L. Ecotoxicology and Environmental Safety 53, 331-347.

Pandey S, Parvez S, Sayeed I, Haque R, Bin-Hafeez B, Raisuddin S. 2003. Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallagoattu (Bl. &Schn.). Science of the Total Environment 309, 105–115.

Paraveen D. 1980.Effect of DDT on digestive tract of Channagachua. IV All India Science Congress, Bhopal University, Bhopal., Abstract No. TC 18.

Parvez S, Raisuddin S. 2006. Effects of Paraquat on the Freshwater Fish Channa punctata (Bloch): Non-Enzymatic Antioxidants as Biomarkers of Exposure. Archives of Environmental Contamination and Toxicology 50, 392–397.

Peebuaa P, Kruatrachuea M, Pokethitiyooka P, Kosiyachindaa P. 2006. Histological Effects of Contaminated Sediments in Mae Klong River Tributaries, Thailand, on Nile tilapia, Oreochromis niloticus. Science Asia 32, 143-150.

Pereira L, Fernandes MN, Martinez CB.2013. Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone. Environmental Toxicology and Pharmacology 36, 1-8.

Prusty AK, Kohli MPS, Sahu NP, Pal AK, Saharan N, Mohapatra S. 2011.Effect of short term exposure to fenvalerate on biochemical and haematological responses in Labeorohita (Hamilton) fingerlings. Pesticide Biochemistry and Physiology 100, 124-129.

Regoli F, Principato G. 1995. Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory condition: implication for the use of biomarkers. Aquatic Toxicology 31, 143–164.

Rodrigues EL, Fanta E.1998.Liver histopathology of the fish Brachydanio rerio after acute exposure to sublethal levels of the organophosphate Dimetoato 500. Revista Brasileira de Zoologia15, 441-450.

Schenker MB, Stoecklin M, Lee K, Lupercio R, Zeballos RJ, Enright P, Hennessy T, Beckett LA. 2004. Pulmonary function and exercise-associated changes with chronic low-level paraquat exposure. American Journal Respiratory and Critical Care Medicine 170, 773–779.

Seiyaboh EI, Inyang IR, Gijo AH, Adobeni GD. 2013. Acute Toxicity of Paraquat Dichloride on Blood Plasma Indices of Clarias gariepinus. IOSR Journal of Environmental Science, Toxicology And Food Technology 7(6), 15-17.

Shah SL, Altindag A. 2004.Haematological parameters of tench, Tincatinca after acute and chronic exposure to lethal and sublethal mercury treatments. Bulletin of Environmental Contamination and Toxicology 73, 911-918.

Shah SL, Altindag A. 2005.Alteration in the immunological parameters of tench (Tincatinca L.) after acute and chronic exposure to lethal and sublethal treatment with mercury, cadmium and lead. Turkish Journal of Veterinary and Animal Sciences 29, 1163-1168.

Shakoori AR, Mughal AL, Iqbal MJ.1996. Effect of sublethal doses of fenvalerate (a synthetic pyrethroid) administered continuously for four weeks on the blood, liver, and muscles of a freshwater fish, Ctenopharyngodon idella. Bulletin of Environmental Contamination and Toxicology 57, 487-494.

Siakpere OK, Adamu KM, Madukelum IT. 2007. Acute haematological effect of sublethal of paraquat on the African catfish, Clarias gariepinus (Osteichthyes: Clariidae). Research Journal of Environmental Sciences 1, 331-335.

Suntres  ZE. 2002.  Role of antioxidants in Paraquat  toxicity. Toxicology 180, 65-77.

Thomaz JM, Martins ND, Monteiro DA, Rantin FT, Kalinin AL. 2009. Cardio-respiratory function and oxidative stress biomarkers in Nile tilapia exposed to the organophosphate insecticide trichlorfon (NEGUVONs). Ecotoxicology and Environment Safety 72, 1413–1424.

Vasylkiv OY, Kubrak OI, Storey KB, Lushchak VI. 2011. Catalase activity as a potential vital biomarker of fish intoxication by herbicide aminotriazole. Pesticide Biochemistry and Physiology 101, 1-5.

Wintrobe MM. 1967. A hematological odyssey,1926–66. The Johns Hopkins Medical Journal 120, 287–309.

Zaragoza A, Andres D, Sarrion D, Cascales M. 2000.Potentiation of thioacetamide hepatotoxicity by phenobarbital pretreatment in rats, inducibility of FAD monooxygenase system and age effect. Chemico-Biological Interactions 124, 87-101.