Study of biochemical and phytochemical parameters in local variety of barley (Hordeum vulgare) under saline conditions

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Research Paper 01/01/2020
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Study of biochemical and phytochemical parameters in local variety of barley (Hordeum vulgare) under saline conditions

Naseem Khatoon, Beena Naqvi, Najum-u-Sahar, Nadir Ali Rind, Muhammad Rafiq, M Umar Dahot
Int. J. Biosci.16( 1), 427-434, January 2020.
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Current research targeted to measure the effect of increasing concentration of (0-200mM) of KCl2 and MgCl2 salt on Hordeum vulgare cultivars, (Jow 83, and Jow 87). Overall, increasing of KCl2 and MgCl2 stress reduced leaf pigments in both cultivars significantly, with a lesser extent in Jow 83 as compared to Jow 87. The present results justify that a significantly (p<0.05 continuously decrease of total sugars, total proteins, antioxidant activity, reducing sugar, phenolic compounds, with increasing of concentration of KCl2 and MgCl2 as compared to control plants. While significantly (p<0.05) increased amount of Proline contents in both cultivars with increasing of salt concentrations as compared to control. Various salt-treated plants showed a decreased amount of various biochemical and phytochemical as compared with control plants. The Jow 87 salt tolerance nature was correlated positively with its better performance in terms of physiological, phytochemical, and biochemical attributes.


Acosta-Motos JR, Ortuño MF, Bernal-Vicente A, Diaz-Vivancos P, Sanchez-Blanco MJ, Hernandez JA. 2017. Plant responses to salt stress: adaptive mechanisms. Agronomy 7(1), 18.

Arzani A, Ashraf M. 2016. Smart engineering of genetic resources for enhanced salinity tolerance in crop plants. Critical Reviews in Plant Sciences 35, 146-189.

Bates LS, Waldran RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39, 205-208.

Brandenbury W, Kleir C. 2011. Effect of MgCl2 on germination, growth and biomass allection of the radish CV. “cherry Belle”. American Journal of Environment Science 7, 132-135.

Cakmak I. 2005. The role of potassium in allevisting detrimental effects of abiotic stress in plants. Journal of Plant Nutrition and Soil Science 168, 521-530.

Danai TS, Kumar V, Shriram V. 2011. Differential Response of two scented Indica Rice (Oryza sativa) Cultivars under salt stress. Journal of Stressphysiology and Biochemistry 7, 387-397.

Dougherty Gk, Smith GR. 2006. Acute effects of road de-icers on the tadpoles of three anurans. Applied herpetology 3, 87-93.

Flowers TJ, Colmer TD. 2008. Salinity tolerance in halophytes. New Phytologist journal 179, 945-963.

Jiang Q, Roche D, ManacoTA, Durham S. 2006. Gas exchange, chlorophyll fluorescence parameters and carbon isotope discrimination of fourteen barley genetic lines in response to salinity, Field Crops Research 96, 269-278.

Kalaji MH, GurP. 2008. Chlorophyll fluorescence: a useful tool in barley plant breeding programs. In: Sanchez A., Gutierrez S.J. (Eds), photochemistry Research progress. Nova publishers, NY, USA pp. 439-463.

Kamali N, Pour MRK, Soleymani AT. 2014. Studying growth indices and grain yield of barley cultivars at planting dates in Isfahan region. International Journal of Farming and Allied Science 3(1), 35-44.

Khare T, Desai D, Kumar V. 2012. Effect of MgCl2 stress on germination, plant growth, chlorophyll content, proline content and lipid peroxidation in Sorghum Cultivars. Journal of Stress Physiology and Biochemistry 8(4),

Kim DO, Jeong SW, Lee CY. 2003. Antioxidant capacity of phenolic, phytochemicals from various cultivars of plums. Food Chemistry journal 81(3), 321-326.

Kumar M, Hasan M, Arora A, Gaikwad K, Kumar S, Rai RD, Singh A. 2015. Sodium chloride-induced spatial and temporal manifestation in membrane stability index and protein profiles of contrasting wheat (Triticum aestivum L.) genotypes under salt stress. Indian Journal of Plant Physiology 20(3), 271-275.

Kumar S, Singh A. 2016. Epigenetic regulation of abiotic stress tolerance in plants. Advances in Plants & Agriculture Research 5, 179.

Lichtenthaler HK, Wellburn AR. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transaction 11, 591-592.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein Measurement with the Follin Phenol Reagent. Journal of Biological Chemistry   193, 265-275.

Machado RMA, Serralheiro RP. 2017. Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulturae 3(2), 30.

Mian A, Oomen RJ, Isayenkov S, Sentenac H, Maathuis FJ, Véry AA. 2011. Over‐expression of an Na+‐and K+‐permeable HKT transporter in barley improves salt tolerance. The Plant Journal 68(3), 468-479.

Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3), 426-428.

Montgomery R. 1961. Further studies of the phenol sulphuric acid reagent for carbohydrates. Biochimica Biophysica Acta pp. 591-593.

Pareek A, Sopory SK, Bohnert HJ, Govindjee (Eds). 2010. Abiotic stress adoption in plants. Physiological, molecular and genomic function springer Dordrecht.

Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ safe 60, 324-349.

Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantification of antioxidant capacity through the formation of phosphomolybdenum complex, specific application to the determination of vitamin E. Analytical Biochemistry 269(2), 337-341.

Qadir M, Quillérou E, Nangia V, Murtaza G, Singh M, Thomas RJ, Drechsel P, Noble A. 2014. November. Economics of salt‐induced land degradation and restoration. In Natural resources forum 38(4), 282-295.

Setter TL. 2003. Waters, I. Review of prospects for germplasm improvement for waterlogging tolerance in wheat and barley and oats. Plants Soil 253-1-34.

Shabala S, Hariadi Y. 2005. Effect of magnesium availability on the activity of plasma membrane ion transporters and light induced response from bread bean leaf mesophyll. Planta 221, 56-65.

Shahabaz M, Ashraf M. 2013. Improving salinity tolerance in cereals. Critical Reviews in Plant Sciences 32, 237-249.

Shaukat S. 2103. Production technology of barley (Hordeum vulgare L.) in Pakistan http// pakgrifarming. Blog production- technology of barley Hordeum.html

Shrivastava P, Kumar R. 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences 22(2), 123-131

Wang M, Zheng Q, Shen Q, Guo S. 2013. The Critical Role of Potassium in Plant Stress Response. International Journal of Molecular Science 14, 7370-7390.

Yasoubi P, Barzegar M, Sahari MA, Azizi MH. 2007. Total phenolic contents and antioxidant activity of Pomegranate (Punica granatum L.) peel extracts. Journal of Agricultural. Science Technology 9, 35-42.