Study of canola (Brassica napus L.) genotypes for salt tolerance at germination stage via multivariate analysis

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Research Paper 01/09/2013
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Study of canola (Brassica napus L.) genotypes for salt tolerance at germination stage via multivariate analysis

Zahra Khodarahmpour, Asieh Soltani, Ali Ashraf Jafari
Int. J. Biosci.3( 9), 109-115, September 2013.
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This study was conducted to evaluate the effect of salt on the germination and early vegetative growth of canola genotypes. In order to an experiment was performed as factorial form under completely randomized design (CRD) with 3 replications. Genotype factor was contains of 12 genotypes and 6 levels of salt (control, -3, -6, -9, – 12 and -15 bar) with NaCl. Results of correlation showed that seedling length had the most positive and significant correlation with index seed vigour (r=0.97**). Factor analysis based on principal component analysis showed that three principle components had eigen values more than 1 and together accounted for 88% of the variability of original data under salt stress. The first component named seedling characteristics and index seed vigour. The second component named mean germination time, plumule length and wet weight of seedling. The three component named germination characteristics and dry weight of seedling. Based biplot, canola genotypes to three groups was divided. The first PCA separated Hyola401 in the extreme horizontal ends of the biplot, Hyola401 expressed as the tolerant genotype in the right and genotypes of H6661 and Q6503 as the most sensitive genotypes in the left side of the biplot.


Abbaszadeh F, Rameeh V, Cherati A. 2012. Salinity stress indices of seed yield and nutrient compositions in Rapeseed (Brassica napus L.). International Journal of Biology 4(1), 154-162.

Ashraf  M.  2004.  Some  important  physiological selection criteria for salt tolerance in plants. Flora-Morphology, Distribution, Functional Ecology of Plants 199, 361-376.

FAO, FAOSTAT. 2011. Available at: (Accessed 15 January, 2011).

Ghaffari M, Toorchi M, Valizadeh M, Shakiba MR. 2012. Morpho-physiological screening of sunflower inbred lines under drought stress condition. Turkish Journal of Field Crops 17(2),185-190

International Seed Testing Association (ISTA) 1996. International rules for seed testing rules. Seed Science and Technology 24, (Supplement.) 155-202.

Janmohammadi M, Moradi Dezfuli P, Sharifzadeh F. 2008. Seed invigoration techniques to improve germination and early growth of inbred line of maize under salinity and drought stress. Genetic Applied Plant Physiology 34, 215-26.

Kaya MD, Day S, Cikili Y, Arslan, N. 2012. Classification of some linseed genotypes for salinity tolerance using germination, seedling growth, and ion content. Chilin Journal of Agricultural Research 72(1), 27-32.

Munns R, James RA, Lauchli A. 2006. Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany 5(57), 1025-1043.

Munns R. 2005. Genes and salt tolerance: bringing them together. New Phytology 167, 645-63.

Murillo-Amador B, Lopez-Aguilar R, Kaya C, Larrinaga-Mayoral J, Flores, HA. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188, 235-47.

Rameeh VO. 2010. Evaluation of genetic variation and correlation among yield and yield components in soybean genotypes using multivariate analysis. Journal of Crop Breeding 2(5), 57-67.

Sharma P, Sardana V, Banga SS. 2013. Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environmental and Experimental Biology 11, 39–46

Zareie S, Mohammadi-Nejad GH, Sardouie-Nasab S. 2013. Screening of Iranian safflower genotypes under water deficit and normal conditions using tolerance indices. Australian Journal of Crop Science 7(7), 1032-1037.