Int. J. Agron. Agri. Res.5(2), 40-45, August 2014
The present study is devoted to the morphological characterization, pathogenicity of Fusarium oxysporum f.sp albedinis, which showed morphological variability of isolates. The study of pectin methyl esterase and polygalacturonase activities by reducing sugars dosages of 10 isolates, showed that the PME and PG activities are present in the culture filtrates of isolates and the estimated amount is much higher in the presence of citrus pectin and glucose. We noted that this activity is variable among isolates, high activity is recorded (1.59 103 U/mole) in presence of glucose but it was greater than 4 times in the presence of citrus pectin, The PG activity is recorded (1.39 103 and 0.44 103μegr) in the presence of 1% citrus pectin and glucose respectively.
Al-Najada AR, Al-Hindi RR, Mohamed SA. 2012. Characterization of polygalacturonases from fruit spoilage Fusarium oxysporum and Aspergillus tubingensis. African Journal of Biotechnology 34, 8527-8536.
Al-Najada AR. 2014. Partial purification and physicochemical characterization of polygalacturonase from Aspergillus awamori. Life Science Journal 5, 253-259.
Armstrong GM, Armstrong JR. 1981. Forms specials and races of Fusarium oxysporum causing wilt diseases. Fusarium : diseases, biology and taxonomy. States Univ. Press. Univ Park and London 3, 391-393.
Belabid L, FORTAS Z. 2002. Virulence and vegetative compatibility of Algerian isolates of Fusarium oxysporum f.sp lentis. Phytopathol. Mediterr 41, 179–187.
El Moudafar C, El Boustani E. 2000. Relationship between cell wall susceptebility to cellulases and pectinases of Fusarium oxysporum and susceptibility of date palm cultivars. Biologia Plantarium 4, 571-576.
Gharbi S, Karkachi, N, Kihal M, Henni J. 2013. Carbon sources and pH effect on pectinolytic activity production by Ascochyta rabiei isolated from chickpea (Cicer arietinum L.) in West Algeria. African Journal of Microbiology Research 7, 3483-3488.
Hamdy HS. 2005. Purification and characterization of the pectin lyase produced by Rhizopus oryzae grown on orange peels. Annals of Microbiology 3, 205-211.
Henni J, Boisson C, Geiger JP. 1994a. Variabilité de la morphologie chez Fusarium oxysporum f. sp. lycopersici . Phytopath. medit 33, 51-58.
Henni J, Boisson C, Geiger JP. 1994b. Variabilité du pouvoir pathogène chez Fusarium oxysporum f. sp. lycopersici . Phytopath. medit 33, 10-16.
Jorge I, Navas-Cortés JA, Jiménez-Diaz RM, Tena M. 2006. Cell wall degrading enzymes in fusarium wilt of chickpea: correlation between pectinase and xylanase activities and disease development in plants infected with two pathogenic races of Fusarium oxysporum f. sp. ciceris. Can. J. Bot 84, 1395–1404.
Lalaoui F, Halama P, Dumortier V, Paul B. 2000. Cell wall-degrading enzymes produced in vitro by isolates of Phaeosphaeria nodorum differing in aggressiveness. Plant Pathology 49, 727-733.
Nitinkumar PP, Bhushan LC. 2010. Production and purification of pectinase by soil isolate Penicillium sp and search better agro-residue for its SSF. Recent Research in Science and Technology 7, 36-42.
Zhang JX, Bruton BD, Biles CL. 2010. Polygalacturonase isozymes produced by Phomopsis cucurbitae in relation to postharvest decay of cantaloupe fruit. Phytopathology 87, 1020-1025
Reignault R, Valette-Collet O, Boccara M. 2008. The importance of fungal pectinolytic enzymes in plant invasion, host adaptability and symptom type. Bulletin OEPP/EPPO 33, 245–247.