Study on the effect of pH and temperature on growth of Pseudomonas sp. in the presence heavy metals

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Research Paper 01/12/2016
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Study on the effect of pH and temperature on growth of Pseudomonas sp. in the presence heavy metals

Naseem Kausar, Samina Qamer
Int. J. Biosci.9( 6), 204-212, December 2016.
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Abstract

The Purpose of present study was to investigate the effect of Cr+2 and Pb+2 on heavy metal tolerant species of Pseudomonas at different pH and temperature for varying incubation periods.It was found that growth of Pseudomonas sp. increased in acidic medium (5.0-6.0) in presence of both metals (Cr+2 and Pb+2).  In presence of chromium metal, Pseudomonas sp. showed maximum growth at pH 6.0. At pH 7.0, bacterial growth was gradually declined in Chromium enriched medium while accelerated up to maximum point in presence of Lead. Therefore, 6.0 and 7.0 were considered optimum pH values for highest bacterial growth in Chromium and Lead containing media respectively. In case of temperature, Pseudomonas sp. exhibited increase in growth only at 50°C in presence of Chromium and remained suppressed at all other selected temperatures (20ºC, 25ºC, 37ºC and 45º). In presence of Lead, Pseudomonas sp. showed significant growth at all selected temperatures (25ºC, 37ºC, 45ºC and 50ºC) except 20ºC. Optimum temperature values found for bacterial growth in Chromium and Lead medium were50ºC and 37ºC, respectively.Under optimum conditions, highest growth of pseudomonas sp. was observed within three days (72 hours) of incubation for both metals.

VIEWS 4

Abouzeid AA, Hassanein WA, Salama HM, Fahd GAA. 2009. Biosorption of Some Heavy Metal Ions Using Bacterial Species Isolated from Agriculture Waste Water Drains in Egypt. Journal of Applied Sciences Research 5, 372-383. www.cabdirect.org/cabdirect/abstract/20093138590

Ajmal M, Mohammad A, Yousuf R, Ahmad A.1998. Adsorption Behaviour of Cadmium, Zinc, Nickel and Lead from Aqueous Solution Mangiferaindica Seed Shell. Indian Journal of Environmental Health 40, 15-26. www.researchgate.net/publication/279553366

Batool  R, Yrjälä K, Hasnain.  S2014.  Impact of environmental stress on the biochemical parameters of bacteria reducing chromium.Brazilian Journal of Microbiology 45(2), 573-583. http://dx.doi.org/10.1590/S151783822014000200028

Bafubiandi AFM,  Dlamini NP, Mamba BB. 2009. Biosorption of Cobalt and Copper from Hydrometallurgical Solutions Mediated by Pseudomonas sp. Hydrometallurgy Conference 2009, The Southern African Institute of Mining and Metallurgy.

Chen P, Ting YP. 1995. Effect of heavy metal uptake on the electrokinetic properties of Saccharomyces cerevisiae. Biotechnology Letters17(1), 107-12. http://dx.doi.org/10.1007/BF00134205

Diels L, Van-der LN, Bastiaens L. 2002.New Development in Treatment of Heavy Metal Contaminated Soils.Reviews in Environmental Science and biotechnology1, 75-82. http://dx.doi.org/10.1023/A:1015188708612

Ensink JHJ, Van-der HW, Matsuno Y, Munir S, Aslam MR. 2002. The Use of Untreated Waste Water in Peri-urban Agriculture in Pakistan: risks and opportunities.Research Report no. 64, International Water Management Institute, Colombo, Sri Lanka, 22 p.

Gabr  RM, Hassan  SHA, Shoreit AAM. 2008. Biosorption of Lead and Nickel by living and non-living cells of Pseudomonas aeruginosa. Int. Biodeter. Biodegr 62, 195-203. http://dx.doi.org/10.1016/j.ibiod.2008.01.008

Gourdon R,  Bhande S, Rus E, Sofer SS. 1990.  Comparison of Cadmium Biosorption by Gram Positive and Gram Negative Bacteria from Activated Sludge.Biotechnology Letters 12, 839-842. http://dx.doi.org/10.1007/BF01022606

Bayramoğlu GBektaş SArica MY. 2003. Biosorption of heavy metal ions on immobilized white-rot fungus Trametesversicolor.Journal of Hazardous Materials 101, 285-300. www.ncbi.nlm.nih.gov/pubmed/12935760

Haq NU, Arain MA, Haque Z, Badar N, Mughal N. 2009. Drinking Water Contamination by Chromium and Lead in Industrial Lands of Karachi.Journal of Pakistan Medical Association 59, 270-274.

Hussein H,  Moawad H, Farag  S. 2004.  Isolation and Characterization of Pseudomonas sppResistant to Heavy Metals Contaminants.Arabic Journal of Biotechnology 7, 13-22.

Kadirvelu K, Thamaraiselvi K, Namasivayam C. 2000. Removal of Heavy Metals from Industrial Wastewaters by Adsorption onto Activated Carbon prepared from an Agricultural Solid Waste. BioresourceTechnolology76, 63-65. http://dx.doi.org/10.1016/S0960-8524(00)00072-9

Kalavrouziotis IK, Koukoulakis PH, Papadopoulos AH. 2009. Heavy metal interrelationships in soil in the presence of treated wastewater. Global Nest Journal, 11(4), 497-509. http://journal.gnest.org/sites/default/files/Journal%20Papers/497-509_578_Kalavrouziotis_11-4.pdf

Kiran I, Akar T, Tunali S. 2005.Biosorption of Pb (II) and Cu (II) from Aqueous Solution by Pretreated Biomass of Neurosporacrassa.Process Biochemistry 40, 3550-3558. http://dx.doi.org/10.1016/j.procbio.2005.03.051

Liu Y, Cao Q, Luo F Chen  J. 2009. Biosorption of Cd+2, Cu+2, Ni+2, and Zn+2 ions from aqueous solutions by pretreated biomass of brown algae. Journal of Hazardous Materials163, 931-938

Mahmood S, Maqbool A. 2006.Impacts of Wastewater Irrigation on Water Quality and on the Health of Local Community in Faisalabad.Pakistan Journal of Water Resources 10, 19-20.

Moghannem SA, Refaat BM,  El-Sherbiny GM, El-SayedMH, Elsehemy IA, Kalaba MH. 2015. Characterization of heavy metal and antibiotic-resistant bacteria isolated from polluted localities in Egypt. Egyptian Pharmaceutical Journal 14,158–165.

Mortazavi S, Rezaee A, Khavanin A, Varmazyar S, Jafarzada M. 2005.Removal of Mercuric Chloride by a Mercury Resistant Pseudomonas putida strain.Journal of Biological Sciences 5(3), 269-273.

Prasenjit B, Sumathi S. 2005.Uptake of Chromium by Aspergillusfoetidus. Journal of Material Cycles of Waste Management 7,88-92.

Prasath  CSS, Manikadan N, Prakash S. 2010. Uranium and Thorium uptake by live and dead cells of Pseudomonas sp. Journal of Scientific and Industrial Research 69, 373-375.

Prescott LM, Harley JP, Klein DA. 2002. Microbiology, Fifth Edition. New York, US. McGraw-Hill Higher Education95-112.

Raji C, Anirudhan TS. 1997. Chromium (VI) Adsorption by Sawdust: Kinetics and Equilibrium. Indian Journal of Chemical Technology 4, 228-236. http://nopr.niscair.res.in/handle/123456789/30928

Raja CE, Anbazhagan K, Selvam GS. 2006. Isolation and Characterization of a Metal-resistant Pseudomonas aeruginosa Strain. World Journal of Microbiology and Biotechnology 22,577-585. http://dx.doi.org/10.1007/s11274-005-9074-4

Rehman K. 1993. Water Consumption Survey for Industries and Institutions in Faisalabad: FDA Report3-5.

Rehman A, Awais SB, Hasnain  S. 2010.  Isolation and Characterization of Arsenite Oxidizing Pseudomonas lubricansand its Potential Use in Bioremediation of Wastewater.African Journal of Biotechnology 9(10), 1493-1498. www.ajol.info/index.php/ajb/article/viewFile/78324/68689

Roane TM. 1999. Lead Resistance in two Isolates from Heavy Metal Contaminated Soils. Microbial Ecology 37, 218–224.

Savvaidis I, Martin NH, Robert KP. 2003.  Copper Biosorption by Pseudomonas cepacia and other Strains. World Journal of Microbiology and Biotechnology 19, 117-121. http://dx.doi.org/10/1023/A:1023284723636

Sengor SS, Gikas P. 2014.  The Influence of Single and combined effects of Zn, Cu and temperature on microbial growth.  Global NEST Journal 16,25-37.

Shetty R, Rajkumar SH. 2009.Biosorption of Cu (II) by Metal Resistant Pseudomonas spp.International Journal of Environmental Research 3(1), 121-128.

Steel RGD, Torrie JH. 1981.  Analysis of Variance factorial experiments. In: Principles and Procedures of Statistics. 2nd Ed, McGraw Hill International Book Company, Tokyo 336-37

Umber L. 2008. Isolation of Heavy Metals Resistant Bacteria from Different Industrial Effluents of Faisalabad and Jhang.M.Sc Thesis.Department of Zoology, GC University Faisalabad, 48-55.

Uzair M, Ahmed M, Nazim K. 2009.Effect of Industrial Waste on Seed Bank and Growth of Wild Plants in Dhabeji area, Karachi, Pakistan.Pakistan Journal of Botany41, 1659-1665. www.pakbs.org/pjbot/PDFs/41(4)/PJB41(4)1659.pdf

Veglio F, Beolchini  F, Gasbarro A. 1997. Biosorption of Toxic Metals: An equilibrium study using free cells of Arthrobactorsp. Process Biochemistry 32, 99-105.

Volesky B. 1993. Biosorption and Biosorbents. In: Biosorption of Heavy Metals. CRC Press, Boca Raton, FL 3-5.

Yan G, Viraraghavan T. 2003. Heavy Metal Removal from Aqueous Solution by Fungus Mucorrouxii. Water Research 37, 4486-4496. http://dx.doi.org/10.1016/S0043-1354(03)00409-3