Sub lethal effects of copper oxide (CuO) nanoparticles on blood parameters of common carp (Cyprinus Carpio)

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Research Paper 01/06/2015
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Sub lethal effects of copper oxide (CuO) nanoparticles on blood parameters of common carp (Cyprinus Carpio)

Mohadeseh Miri, Abdolali Rahdari
J. Bio. Env. Sci.6( 6), 283-291, June 2015.
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Abstract

Nanotechnology is identifying and controlling materials in dimensions between 1-100 nanometers as physical, chemical and biological properties of material are unusual in these dimensions. The concerns about probable dangers of releasing nanoparticle materials to the environment are increasing with increasing development of this technology. The objective of this study is to examine the effect of nanoparticle copper oxide and AST and ALT enzymes in the blood serum of common carp. In this research, six groups of sixteen carp (Cyprinus carpio) consisting of a control group and five groups are exposed to nanoparticles concentrations of 5, 10, 50, 100 and 150 µg.l-1 after which bleeding was done after 24, 48, 72 and 96 hours. AST and ALT enzymes, protein, urea, and glucose in the blood serum were measured by auto-analyzer and cortisol by commercial kits. The results showed that the amount of AST and ALT enzymes, glucose, cortisol and urea significantly (p<0/05) increased in the blood of carps exposed to nanoparticles compared to control fish. Also, significant differences (p<0/05) were observed between quoted and basic factors at various periods of time, but protein did not show significant differences (p>0/05) in comparison with the control and 5 other. Groups Carps reacted to nanoparticle copper in the environment and it has been possible to use measured factors such as AST and ALT enzymes as the pollution index of an environment with nanoparticle copper.

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