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Susceptibility of Nilaparvata lugens (Stal.) to a botanical insecticide formulation of Neem (Azadirachta indica A. Juss) and Citronella Oil (Cymbopogon nardus L. Rendle) mixture

Neneng Sri Widayani, Danar Dono, Yusup Hidayat

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Int. J. Biosci.14(2), 593-599, February 2019

DOI: http://dx.doi.org/10.12692/ijb/14.2.593-599


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Nilaparvata lugens (Stal.) is one of the main insect pests of rice plant in Indonesia. The farmer usually using synthetic insecticide to control this pest insect. However, unwise of using insecticides cause many negative impact such as resistance, resurgence, environmental contamination, and healthy problem. These were encouraged to search and development of natural resources origin from plant for insect pest control. Neem (Azadirachta indica) and citronella (Cymbopogon nardus) have insecticidal activity and safety for the environment. This study aimed to compare the susceptibility of N. lugens from Cipunagara (field population) and Banyuwangi (standard population) to the insecticide formulation made from neem and citronella oil mixture (50:15). The experiments used leaf-stem dipping and root dipping methods. N. lugens instar 4th were exposed to the treated rice plant for two days and after that they were provided with rice plants without treatment until ten days. The results showed that the LC50 value in the root dipping method was lower than that in the leaf-stem dipping method. In this test, the toxicity of neem and citronella oil mixture formulation was higher in the root dipping method and was effective in controlling N. lugens in both populations with the LC95 values were 2.09%-2.30%. The Resistance Ratio (RR) of N. lugens from Cipunagara population and from Banyuwangi population were < 1 in both leaf-stem dipping and root dipping tests, respectively. Therefore, N. lugens from Cipunagara population is still susceptable to neem and citronella oil mixture formulation.


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Susceptibility of Nilaparvata lugens (Stal.) to a botanical insecticide formulation of Neem (Azadirachta indica A. Juss) and Citronella Oil (Cymbopogon nardus L. Rendle) mixture

Akol AM, Sithanantham S, Njagi P GN, Varela A, Mueke JM. 2002. Relative safety of sprays of two neem insecticides to Diadegma mollipla (Holmgren), a parasitoid of the diamondback moth: Effects on adult longevity and foraging behaviour. Crop Protection 21, 853-859. https://doi.org/10.1103/ PhysRevC.87.054310

Alam MM, Ahmad M, Khan AM. 1980. Effect of organic amendments on the growth and chemical composition of tomato, eggplant and chilli and their susceptibility to attack by Meloidogyne incognita. Plant and Soil 57, 231-236. https://doi.org/ 10.1007/BF02211683.

Arisanti IM, Dono D. 2015. Bioactivity mixture of barringtonia asiatica L. (Kurz.) Seed extract (lecythidaceae) and azadirachta indica A. Juss gum (meliaceae) against litura Spodoptera larvae F. (lepidoptera: noctuidae). Jurnal Agrikultura 26, 30-40 (In Indonesian with an English abstract).

Baehaki SE. 2012. Changing of brown planthopper biotype on rice crop. Iptek Tanaman Pangan 7, 8-17 (In Indonesian with an English abstract).

Baehaki SE, Widiarta I. 2011. Planthoppers and how to control the rice plant. Inovasi Teknologi Produksi Padi 347-383 (In Indonesian).

Birgucu AK, Ozger S, Pohl D, Karaca I. 2018. Effects of soil application of neem on some biological characteristics of Myzus persicae (Sulzer) (Hemiptera: Aphididae). Journal of Agricultural Science 24, 256-268.

Cloyd RA. 2010. Pesticide mixtures and rotations: are these viable resistance mitigating strategies ?. Pest Technology 4, 14-18.

Ganjewala D. 2009. Cymbogon essential oils: Chemical compositions and bioactivites. International Journal of Essential Oil Therapeutics 3, 56-65.

ICRR (Indonesian Center for Rice Research). 2017. Reality Attack Brown plant hopper (BPH) in Cipunagara District, Subang Regency. Available at http://bbpadi.litbang.pertanian.go.id/index.php/info-berita/berita/realitas-serangan-ha ma-wereng-batang-coklat-wbc-di-kecamatan-cipunagara-kabupaten-subang. Accessed on 27 June 2017 (In Indonesian).

Javed N, Gowen SR, Inam-ul-Haq M, Abdullah K, Shahina F. 2007. Systemic and persistent effect of neem (Azadirachta indica) formulations against root-knot nematodes, Meloidogyne javanica and their storage life. Crop Protection 26, 911-916. https://doi.org/10.1016/j.cropro.2006.08.011.

Koul O, Singh G, Singh R, Singh J, Daniewski WM, Berlozecki S. 2004. Bioefficacy and mode-of-action of some limonoids of salannin group from Azadirachta indica A. Juss and their role in a multicomponent system against lepidopteran larvae. Journal of Biosciences 29, 409-416.  https://doi.org/10.1007/BF02712112.

Kumar P, Poehling HM, Borgemeister C. 2005. Effects of different application methods of azadirachtin against sweetpotato whitefly Bemisia tabaci Gennadius (Hom., Aleyrodidae) on tomato plants. Journal of Applied Entomology 129, 489-497. https://doi.org/10.1111/j.1439-0418.2005.01009.x.

Lowery DT, Smirle MJ. 2000. Toxicity of insecticides to obliquebanded leafroller, Choristoneura rosaceana, larvae and adults exposed previously to neem seed oil. Entomologia Experimentalis et Applicata 95, 201-207.  https://doi.org/10.1023

Mordue (Luntz) AJ, Nisbet AJ. 2000. Azadirachtin from the neem tree Azadirachta indica: its action against insects. Anais Da Sociedade Entomológica Do Brasil 29, 615-632. https://doi.org/10.1590/S0301.

Nasiruddin M, Mordue(Luntz) AJ. 1993. The effect of azadirachtin on the midgut histology of the locusts, Schistocerca gregaria and Locusta migratoria. Tissue and Cell 25, 875-884. https://doi.org/10.1016/0040-8166(93)90036-K.

Pathak CS, Tiwari SK. 2017. Potential of neem seed’s acetone extract on the haemolymph and fat biochemistry of Cocyra cephalonica larvae (Lepidoptera: Pyralidae). Journal Adv. Zoologi 38, 164-177.

Pavela R, Žabka M, Kalinkin V, Kotenev E, Gerus A, Shchenikova A, Chermenskaya T. 2013. Systemic Applications of Azadirachtin in the Control of Corythucha ciliata (Say, 1832) (Hemiptera:Tingodae), a pest of Plantanus sp. Plant Protection Science 49, 27-33.

Saxena RC, Khan R, Bajet NB. 1987. Reduction of Tungro Virus Transmission by . Nephotettix virescens (Homoptera: Cicadellidae). Journal of Economic Entomology 80, 1079-1082.

Senthil-Nathan S, Choi MY, Paik CH, Seo HY, Kim JD, Kang SM. 2007. The toxic effects of neem extract and azadirachtin on the brown planthopper, Nilaparvata lugens (Stål) (BPH) (Homoptera: Delphacidae). Chemosphere 67, 80-88. https://doi.org /10.1016/j.chemosphere.2006.09.045.

Surahmat EC, Dadang, Prijono D. 2016. Susceptibility of Brown Planthopper (Nilaparvata lugens) Origin From Six Locations in Java Against Three Kinds of Insecticide. Jurnal Hama Dan Penyakit Tumbuhan Tropika 16, 71-81 (In Indonesian and English abstract).

Suryaningsih E, Hadisoeganda AWW. 2007. Control measure for important pest and diseases of hot pepper by applying biorational pesticides. Jurnal Hortikultura 17, 261-269 (In Indonesian and English abstract).

Wang H, Lai D, Yuan M, Xu H. 2014. Growth inhibition and differences in protein profiles in azadirachtin-treated Drosophila melanogaster larvae. Electrophoresis 35, 1122-1129. https://doi.org /10.1002/elps.201300318.

Watanabe T, Kitagawa H. 2000. Photosynthesis and translocation of assimilates in rice plants following phloem feeding by the planthopper Nilaparvata lugens (Homoptera : Delphacidae ). Journal Economi Entomology 93, 1192-1198.

Weathersbee AA, McKensie CL. 2005. Effect of a neem biopesticide on repellency, mortality, oviposition, and development of Diaphorina citri (Homoptera : Psyllidae). Florida Entomologist 88, 401-407. https://doi.org/10.1653/0015-4040(2005)

Zanuncio JC, Mourão SA, Martínez LC, Wilcken CF, Ramalho FS, Plata-Rueda A, Serrão JE. 2016. Toxic effects of the neem oil (Azadirachta indica) formulation on the stink bug predator, Podisus nigrispinus (Heteroptera: Pentatomidae). Scientific Reports 6, 1-8. https://doi.org/10.1038/srep30261.