The agrA expression linked to quorum sensing-mediated coagulase formation using philippine ilongot ethnobotanicals against Staphylococcus aureus

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Research Paper 01/08/2019
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The agrA expression linked to quorum sensing-mediated coagulase formation using philippine ilongot ethnobotanicals against Staphylococcus aureus

Gabrielle T. Salamanca, Somar Israel D. Fernando, Khristina G. Judan Cruz
Int. J. Biosci.15( 2), 33-41, August 2019.
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Staphylococcal infections are among the most common hospital-acquired infections worldwide. Quorum sensing in bacterium is vital to their pathogenicity and the use of quorum sensing inhibition (QSI) compounds reveals opportunities to target virulence without developing resistance and suggests a strategic approach to develop drugs against pathogenic bacteria.  Ethnobotanical crude extracts (ECEs) of the Ilongot community of Aurora province, Philippines were tested for the QSI activity against coagulase formation in the reference strain Staphylococcus aureus. Antibacterial testing of extracts was done as pre-screening for coagulase formation.  Extracts were evaluated through tube coagulase assay for coagulase formation. Molecular confirmation of quorum sensing inhibition as affected by the ECEs was done through gene expression analysis of agrA. Antibacterial activity was observed in Urena lobata leaves, Dillenia philippinensis leaves and Diplazium esculentum. Coagulation was inhibited in tubes treated with crude extracts of Dillenia philippinensis bark, Stachytarpeta jamaicensis leaves, and Eleusine indica roots therefore, exhibiting QSI activities against S. aureus. S. jamaicensis leaves. E. indica roots and D. philippinensis bark significantly downregulated agrA therefore, confirming the QSI activity. The results indicate the potential of these ethnobotanicals for therapeutic approach in inhibiting bacterial virulence without developing resistance.


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