The aplication of mutation induction by gamma irradiation on cultivars yam (Dioscorea alata L.) from banggai islands, Indonesia

Paper Details

Research Paper 01/08/2014
Views (174) Download (3)

The aplication of mutation induction by gamma irradiation on cultivars yam (Dioscorea alata L.) from banggai islands, Indonesia

Aser Yalindua, Sudarsono, Asep Setiawan, H.M.H. Bintoro
Int. J. Agron. Agri. Res.5( 2), 46-54, August 2014.
Certificate: IJAAR 2014 [Generate Certificate]


This study aims to obtain mutant plants through induced mutations gamma rays. Materials research 20 accessions of yam from Banggai Islands, Indonesia, which were irradiated with doses of 0 Gy, 10 Gy, 20 Gy, and 30 Gy using a Gamma Chamber 4000 A. The results showed the effect of growing LD50 at 0 Gy (100%), 10 Gy (88.33% ), 20 Gy (86.67%), and 30 Gy (73.33%). Effect of irradiation dose of 20 Gy and 30 Gy causes accession BDa-05 and BDa-24 on the generation of MV1, MV2 and MV3, growing spread on the soil surface. Dose of 30 Gy irradiation led to the accession the round shape yam (BDa-30) into length the MV1, MV2 and MV3 generation. MV1 and MV2, shows the effect of irradiation dose of 30 Gy was significant different from 0 Gy and 10 Gy for duration of growth. Factors yam plant accessions and gamma radiation dose factors of significant influence on the length of growing crops, tuber number, stem diameter, length and weight of tubers. Exposure to gamma radiation doses higher tends to decrease the number of tubers, stem diameter, length and weight of tubers on MV1 generation. Exposure to irradiation 20 Gy and 30 Gy may cause growth patterns accession uwi some changes from pole climbing into climbing above the soil surface. Exposure to 30 Gy irradiation led to form the BDa-30 uwi accession changed (mutated) from the previous round shape becomes elongated.


Abdul M, Asif UK, Habib A, Zahir M. 2010. Gamma Irradiation Effects on Some Growth Parameters of Lepidium sativum L. ARPN Journal of Agricultural and Biological Science. (5)1, Januari 2010.

Anchalee J. 2011. Morphological Change Due to Effects of Acute Gamma Ray on Wishbone Flower (Torenia fourmieri) In Vitro. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies. International Transaction Journal of Engineering, Volume 2(4), ISSN 2228–9860.

Bhosale UP, Hallale BV. 2011. Gamma Radiation Induced Mutations in Black gram (Vigna mungo (L.) Hepper). Asian Journal of Plant Science and Research, 2011 1(2), 96–100.

Datta SK. 2009. A Report on 36 Years of Practical Work on Crop Improvement Through Induced Mutagenesis. In: Induced Plant Mutations in the Genomics Era, Shu, Q.Y. (Ed.). Food and Agriculture Organization of the United Nations, Rome, 253–256 p.

Dhakshanamoorthy D,  Selvaraj R, Chidambaram A. 2010. Physical and chemical mutagenesis in Jatropha curcas L. To induce variability in seed germination, growth and yield traits. Rom. J. Biol. Plant Biology 55, 113–125.

Dhakshanamoorthy D, Selvaraj R, Chidambaram ALA. 2011. Induced mutagenesis in Jatrophacurcas L. Using γ rays and detection of DNA polymorphism through RAPD marker. Comptes Rendus. Biol. 334, 24–30.

Dwimahyani I, Ishak. 2004. Induced mutation on Jatropha (Jatropha curcas L.) for improvement of agronomic characters variability. Atom. Indonesia J., 30, 53–60.

Ellafa K, Ahmed OH, Shaharudin S, Rahman DA. 2007. Gamma radio sensitivity study on snap bean (Phaseolus vulgaris). Int. J. Agric. Res., 2, 844– 848.

James I, Onimisi MY, Jonah SA. 2012. Effect of Irradiation on Sprouting of Water Yam (Dioscorea alata L.) Using Different Doses of Gamma Radiation. American Journal of Chemistry 2012, 2(3), 137–141.

Khan S, Goyal S. 2009. Improvement of mungbean varieties through induced mutations. Afr. J. Plant Sci. 3, 174–180.

Mark J, Bassett. 1992. Characterization and Inheritance of Four Induced Leaf Mutants in Common Bean. J. AMER. Sot. HORT. SCI. 117(3), 512–514. 1992.

Nur I, Septin PA. 2006. Processing statistic data with ease used MINITAB 14. CV. Andi Offset, Yogyakarta 5528. Indonesia.

Nwachukwu  EC,  Mbanaso  ENA,  Nwosu  KI. 2009. The Development of New Genotypes of the White Yam by Mutation Induction Using Yam Mini-tubers . Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome, 2009, 309–312.

Gnanamurthy S, Mariyammal S, Dhanavel D, Bharathi T. 2012. Effect of gamma rays on yield and yield components characters R3 generation in cowpea (Vigna unguiculata (L.) Walp). International Journal of Research in Plant Science 2012; 2(2), 39– 42.

Palamine MTL, Rommel GAC, Lucia JM, Avelina GL, Zenaida BD, Custer CD. 2005. Some Biophysical Changes in the Chloroplasts of a Dracaena Radiation-Mutant. Philippine Journal of Science 134(2), 121-126, December 2005 ISSN 0031–7683.

Robert GDS, James HT. 1991. Prinsiples and Prosedur of Statistic. Ed – 2 PT. Gramedia Pustaka Utama, Anggota IKAPI Jakarta, 1989.

Rong-Li WU, Hong H, Yan-Ling Z, Xing L, Xiao-Hua L. 2008. The effect of ~(60)COγ irradiation on culture in vitro of Pogostemoncablin cablin (BLANCO) Benth. J. Nucl. Agric. Sci. 22, 14– 17.