The bactericidal effect of the Nd: YAG laser on the bacteria of the infected root canals: an in vitro study

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Research Paper 01/11/2018
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The bactericidal effect of the Nd: YAG laser on the bacteria of the infected root canals: an in vitro study

Hareth A. Alrikaby, Balsam S. Abdulhameed
Int. J. Biosci.13( 5), 483-490, November 2018.
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It is very important to obtain the maximum sterilization and antibacterial effect during treatment of the infected root canals. This study aimed to explore the bactericidal effect of the Nd:YAG laser.  The method basically is based on irradiating a bacterial sample by Nd:YAG laser and calculating the number of colony forming units (that will appear on agar plates) before and after laser application. 30 swabs were taken from infected root canals immediately after tooth extraction and immersed in a tubes containing liquid nutritive media. These swabs undergone a serial dilution and then streaked on a blood agars and incubated. After 24 hours, the colony forming units were calculated. The sample (in the tube) then irradiated by laser, and also streaked on blood agar and incubated. The colony forming units were calculated again. Nd:YAG laser showed a weak bactericidal effect with about 25% of the bacterial cells were eradicated. based on the results of this study, the Nd:YAG laser was merely effective as a bactericidal agent.


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