The brine shrimp toxicity of the philippine marine sponges Axinyssa sp., Plocamionida sp., Forcepia sp., Pachymatisma sp., and Placospongia sp.

Paper Details

Research Paper 01/03/2019
Views (570)
current_issue_feature_image
publication_file

The brine shrimp toxicity of the philippine marine sponges Axinyssa sp., Plocamionida sp., Forcepia sp., Pachymatisma sp., and Placospongia sp.

Sari Rose S. Murcia, Ephrime B. Metillo, Mylene M. Uy
Int. J. Biosci. 14(3), 30-34, March 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Marine sponges have been considered as a gold mine with respect to the diversity of their secondary metabolites and biological activities. Five marine sponges were collected off Agusan del Norte, Philippines and taxonomically identified as Axinyssa sp., Plocamionida sp., Forcepia sp., Pachymatisma sp., and Placospongia sp.  Nonpolar and polar extracts of the marine sponges were prepared by sequential partitioning of their freeze-dried samples with 1:1 ethyl acetate-methanol and 1:1 ethanol-water, respectively.  The toxicity of the sponge extracts was evaluated against the brine shrimp Artemia salina after six (6) and twenty-four (24) hours exposure to indicate acute and chronic toxicity, respectively. The results indicated significant brine shrimp toxicity for most of the marine sponge extracts. The highest potencies were exhibited by the nonpolar and polar extracts of Forcepia sp. (acute LC50 = 22.24 g/mL; chronic LC50 = 3.98 g/mL), the polar extract of Axinyssa sp. (chronic LC50 = 17.90 g/mL), and the polar extract of Placospongia sp. (chronic LC50 = 4.48 g/mL). Accordingly, these active sponge extracts are worthy of further investigation for the isolation and determination of potential anti-tumor and pesticidal compounds.

Ebada S, Lin W, Proksch P. 2010. Bioactive Sesterterpenes and Triterpenes from Marine Sponges: Occurrence and Pharmacological Significance. Marine Drugs 8(2), 313-346. http://dx.doi.org/10.3390/md8020313

Davis A. 2006. Medicines by Design. National Institute of Health 6(272), 28.

Haefner B. 2003. Drugs from the deep: marine natural products as drug candidates. Drug Discovery Today 8(12), 536-544.

Jirge S, Chaudhari Y. 2010. Marine: The ultimate source of bioactives and drug metabolites. International Journal of Research in Ayurveda and Pharmacy 1(1), 55-62.

Sipkema D, Franssen MCR, Osinga R, Tramper J, Wijffels RH. 2005. Marine Sponges as Pharmacy. Marine Biotechnology 7(3), 142-162.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Journal of Medicinal Plants Research 45, 31-34. http://dx.doi.org/10.1055/s-2007-971236

(Hooper JNA, Soest RWM Van (eds). 2002. Systema Porifera. A Guide to the classification of sponges Vols 1&2. Kluwer Academic/ Plenum Publishers: New York, Boston, Dordrecht, London, Moscow. Xlviii, p 1708.

Reed LJ, Muench H. 1938. A simple method of estimating fifty percent endpoints. American Journal of Epidemiology 27(3), 493-497.

Krishnaraju AV, Rao TVN, Sundararaju D, Vanisree M, Tsay H, Subbaraju GV. 2005. Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering 3(2), 125-134.

Cordell GA, Kinghorn AD, Pezzulo JM. 1993. Separation, structure elucidation and bioassay of cytotoxic natural products. Bioactive Natural Products. Taylor & Francis, LLC: CRC Press; Boca Raton Florida, USA., p 195-216.

Wright AE, Chen Y, Winder PL, Pitts TP, Pomponi SA, Longley RE. 2004. Lasonolides C-G, Five New Lasonolide Compounds from the Sponge Forcepia sp. Journal of Natural Products 67(8), 1351-1355. http://dx.doi.org/10.1021/np040028e

Zubia E, Ortega MJ, Guerrero CJH, Carballo JL. 2008. Isothiocyanate Sesquiterpenes from a Sponge of the Genus Axinyssa. Journal of Natural Products 71(12), 608-614. http://dx.doi.org/10.1021/np800465n

Kim H, Chin J, Choi H, Baek K, Lee T, Park SE, Wang W, Hahn D, Yang I, Lee J, Mun B, Ekins M, Nam S, Kang H. 2013. Phosphoiodyns A and B, Unique Phosphorous-Containing Iodinated Polyacetylenes from a Korean Sponge Placospongia sp. Organic Letters 15(1), 100-103. http://dx.doi.org/10.1021/ol3031318

Minto RE, Blacklock BJ. 2008. Biosynthesis and Function of Polyacetylenes and Allied Natural Products. Progress in Lipid Research 47(4), 233-306. http://dx.doi.org/10.1016/j.plipres.2008.02.002

Youssef DTA, Van Soest RWM, Fusetani NJ. 2003. Callyspongamide A, a new cytotoxic polyacetylenic amide from the Red Sea sponge Callyspongia fistularis. Natural Product Research 66, 861-862. http://dx.doi.org/10.1080/14786419.2016.1155577

Faulkner DJ. 1997. Marine Natural Products. Natural Product Reports 14, 259-302. http://dx.doi.org/10.1039/NP9971400259

Related Articles

Yield performance assessment of different mustard cultivars under field conditions

Md. Khan Jahan Ali, Md. Moshiur Rahman, Kamrun Nahar, Sharmin Ara Jannat, Mst. Khadija Khatun, Sushan Chowhan, Md. Habibur Rahman, Int. J. Biosci. 27(2), 277-282, August 2025.

Species composition of xylomicobiota of some woody plants distributed in Azerbaijan

K. F. Bakhshaliyeva, V. Y. Hasanova, N. R. Namazov, B. N. Aliyeva, S. C. Garayeva, S. E. Nagiyeva, P. Z. Muradov, Int. J. Biosci. 27(2), 267-276, August 2025.

Perception of producers and processors on sorghum diversity in the context of climate change in center and Northern Benin

Parfait Segla Alohoutade1,2,3, Alphonse Sako Avocefohoun*1,2,3, Sènan Vodouhe2, Mohamed Kanazoe1,2,3, Nicodème Chabi1, Lamine Said Baba-Moussa3, Int. J. Biosci. 27(2), 256-266, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 243-255, August 2025.

Epizootic status and eradication of parasitic diseases in brown bears transferred to the rehabilitation center in Azerbaijan

Siala İ. Rustamova, Aygun A. Azizova, Gular R. Mammadova, Ramin S. Mammadov, Int. J. Biosci. 27(2), 236-242, August 2025.

Cytotoxic and apoptotic effects of Annona squamosa (Atis) crude leaf extract against A549 (Human Lung Adenocarcinoma) cell line

Ashton U. Lim, Noricel U. Garcia, Alkauzar H. Tantong, Int. J. Biosci. 27(2), 226-235, August 2025.

Perceptions of stakeholders towards sheep and goat dairy products in Benin

Yvette Adje, Philippe Sessou, Aretas Tonouhewa, Paulin Azokpota, Lamine Baba-Moussa, Souaïbou Farougou, Int. J. Biosci. 27(2), 211-225, August 2025.

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.