The comparative effects of Artemisia deserti extract and diazinon on rat liver

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Research Paper 01/06/2015
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The comparative effects of Artemisia deserti extract and diazinon on rat liver

Leila Amjad, Ali Noori, Fereshteh Yazdani, Ali Akbar Malekirad
J. Bio. Env. Sci.6( 6), 68-75, June 2015.
Certificate: JBES 2015 [Generate Certificate]

Abstract

Artemisia is a diverse genus of Asteraceae family that has pharmacological effects and it used for treat diseases, including antioxidant effects against oxidative stress. This study investigated the comparative effects of Artemisia deserti extract and diazinon. A. deserti was collected from Isfahan, Iran then 20 g of flower powder was extracted with 150 mL 80% ethanol and the 100, 200 mg/kg concentrations of ethanolic extract were prepared. The animals were divided in to 6 groups: 1. control, 2. diazinon, 3 and 4. diazinon and extract (100, 200 mg/kg), 5 and 6. extract (100, 200 mg/kg). The blood samples were collected and the rate of AST, ALT, ALP, HDL, LDL, serum total antioxidant and MDA were assayed. Also, the liver tissue was isolated for histopathological examination. Finally, the statistical comparisons were done with ANOVA test. The rate of ALT and MDA were changed significantly in some groups. Moreover, results indicated tissue disorders in all groups compared to control, including the accumulation of blood and Kupffer cells in central veins and sinusoidal spaces, necrosis of hepatocytes and damage of central vein. Diazinon cause oxidative stress and liver disorders, similar to the effect of artemisinin on liver; therefore, simultaneous use of these compounds could enhance the toxic effects.

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Adam S, AI-Qarawi A, Elhag E. 2000. Effects of various levels of dietary Artemisia abyssinica leaves on rats. Laboratory Animals 34, 307-312.

Aragon G, Younossi ZM. 2010. When and how to evaluate mildly elevated liver enzymes in apparently healthy patients. Cleveland Clinic Journal of Medicine 77, 195-204.

Ahmad Z. 2011. Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. American Journal of Botany 10, 13852-13859.

Amat N, Upur H, Blazekovic B. 2010. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. Journal of Ethnopharmacology 131, 478-484.

Amirkabirian N, Teimouri F, Esmaily H, Mohammadirad A, Aliahmadi A, Abdollahi M. 2007. Protection bypentoxifylline ofdiazinon-induced toxic stress in rat liver andmuscle. Toxicology Mechanisms and Methods 17, 215-221.

Atawodi SE, Ene AC,  Ameh DA. 2011. Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses. Human & Experimental Toxicology 30, 25-33.

Benzi IF, Strain JJ. 1999. Ferric reducing antioxidant assay. Methods Enzymol 292, 15-27.

Chaturvadi D. 2011. Sesquiterpene lactones: Structural diversity and their biological activities. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. India: Research Signpost 313-334.

El-Shenawy NS, Al-Eisa RA, El-Salmy F, Salah O.  2009.  Prophylactic  effect  of  vitamin  E  against hepatotoxicity, nephrotoxicity, haematological induces and histopathology induced by diazinoninsecticide in mice. Current Zoology 55, 219-226.

Esterabeur H, Cheeseman KH. 1990. Determination of aldehyids lipid peroxidation products: malondealdhyde and 4-hydroxyl nonenal. Methods in Enzymology 186, 407-421.

Ferreira JFS, Luthria DL, Sasaki T, Heyerick A. 2010. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules Journal 15, 3135-3170.

Gilani AH, Janbaz KH. 1993. Protective effect of Artemisia scoparia extract against acetaminophen-induced hepatotoxicity. General Pharmacology: The Vascular System 24, 1455-8.

Jia X, Zhao CH, Chen Y. 2010. The in vitro and in vivo antioxidant activities of various extracts from ArtemisisaselengensisTurcz. Recent Advances in Clinical Medicine 261-266.

Kazemi M, Shafizadeh S, Larijani K. 2011. Comparison of essential oils composition of stem, leaf and flower from Artemisia deserti kracsh. Journal of Applied Chemical Research 18, 29-34.

Kalender S, Ogutcu A, Uzunhisarcikli M, Acikgoz F, Durak D. 2005. Diazinon–induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology 211, 197-206.

Kim KS, Lee MY. 1996. Effects of Artemisia selengensis methanol extract on ethanol induced hepatotoxicity in rat liver. Korean Society of Food and Nutrition 25, 581-587.

Kalantari H, Galehdari H, Zaree Z, Gesztelyi R, Varga B, Haines D, Bombicz M, Tosaki A, Juhasz B. 2013. Toxicological and mutagenic analysis of Artemisia dracunculus (tarragon) extract. Food and Chemical Toxicology 51, 26-32.

Karakoca K, Ozusaglam MA, Cakmak YS, Erkul SK. 2013. Antioxidative, antimicrobial and cytotoxic properties of Isatis FloribundaBoiss. Ex Bornm. extracts. EXCLI Journal 12, 150-167.

Lee LK, Gan V, Lee V, Tan A, Leo Y, Lye D. 2012. Clinical relevance and discriminatory value of elevated liver aminotransferase levels for dengue severity. Public Library of Science 6, 1-8.

Lateef-Molan A, Mohamad-Faraj A, Saleh Mahdy A. 2012. Antioxidant activity and phenolic content of some medicinal plants traditionally used in northern Iraq. The Journal of Phytopharmacology 2, 224-233.

Moujerloo M. 2010. Variations of lipid peroxidation and superoxide dismutase activity due to haemodialysis in gorgan. Journal of Clinical and Diagnostic Research 4, 2763-2767.

Nahrevanian H,  Sheykhkanlooye-Milan B, Kazemi M, Hajhosseini R, Soleymani-Mashhadi S, Nahrevanian SH. 2012. Antimalarial effects of iranian flora Artemisia sieberi on Plasmodium berghei invivo in mice and phytochemistry analysis of its herbal extracts. Malaria Research and Treatment 2012, 1-8.

Nyblom  H, Berggren U, Balldin J, Olsson  R. 2004. High AST/ALT ratio may indicate advanced alcoholic liver disease rather than heavy drinking. Alcohol and Alcoholism 39, 336-339.

Noori A, Amjad L, Yazdani F. 2014. The effects of Artemisia deserti ethanolic extract on pathology and function of rat kidney. Avicenna Journal of Phytomedicine 4, 371-376.

Rezaei A, Shekar-Foroush SH, Changizi-Ashtiyani S, Aqababa H, Zarei A, Azizi M, Yarmahmodi H. 2013. The effects of Artemisia aucheri extract on hepatotoxicity induced by thioacetamide in male rats. The American Journal of Psychiatry 3, 1-9.

Razavi M, Hosseinzadeh H, Abnous KH, Motamedshariaty VS, Imenshahidi M. 2013. Crocin restores hypotensive effect of subchronic administration of diazinon in rats. Iranian Journal of Basic Medical Sciences 16, 64-72.

Sarhan OM, Al-Sahhaf ZY. 2011. Histological and biochemical effects of diazinon on liver and kidney of rabbits. Life Science Journal 8, 1183-1189.

Temraz A, El-Tantawy WH. 2008. Characterization of antioxidant activity of extract from Artemisia vulgaris. Pakistan Journal of Pharmaceutical Sciences 21, 321-6.

Thapa BR, Walia A. 2007. Liver function tests and their interpretation. Indian Journal of Pediatrics 74, 663-671.

Ugusman A, Zakaria Z, KienHui CH, MohdNordin NAM, Mahdy ZA. 2012. Flavonoids of piper sarmentosum and its cytoprotective effects against oxidative stress. EXCLI Journal 11, 705-714.