The comparative effects of Artemisia deserti extract and diazinon on rat liver

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Research Paper 01/06/2015
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The comparative effects of Artemisia deserti extract and diazinon on rat liver

Leila Amjad, Ali Noori, Fereshteh Yazdani, Ali Akbar Malekirad
J. Bio. Env. Sci.6( 6), 68-75, June 2015.
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Artemisia is a diverse genus of Asteraceae family that has pharmacological effects and it used for treat diseases, including antioxidant effects against oxidative stress. This study investigated the comparative effects of Artemisia deserti extract and diazinon. A. deserti was collected from Isfahan, Iran then 20 g of flower powder was extracted with 150 mL 80% ethanol and the 100, 200 mg/kg concentrations of ethanolic extract were prepared. The animals were divided in to 6 groups: 1. control, 2. diazinon, 3 and 4. diazinon and extract (100, 200 mg/kg), 5 and 6. extract (100, 200 mg/kg). The blood samples were collected and the rate of AST, ALT, ALP, HDL, LDL, serum total antioxidant and MDA were assayed. Also, the liver tissue was isolated for histopathological examination. Finally, the statistical comparisons were done with ANOVA test. The rate of ALT and MDA were changed significantly in some groups. Moreover, results indicated tissue disorders in all groups compared to control, including the accumulation of blood and Kupffer cells in central veins and sinusoidal spaces, necrosis of hepatocytes and damage of central vein. Diazinon cause oxidative stress and liver disorders, similar to the effect of artemisinin on liver; therefore, simultaneous use of these compounds could enhance the toxic effects.


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