Paper Details

The comparison between effects of free curcumin and curcumin loaded PLGA-PEG on telomerase and TRF1 expressions in calu-6 lung cancer cell line

Mortaza Taheri Anganeh, Fatemeh Sadat Tabatabaei Mirakabad, Mahmoud Izadi, Vahideh Zeighamian, Fariba Badrzadeh, Roya Salehi, Nosratollah Zarghami, Masoud Darabi, Abolfazl Akbarzadeh, Mohammad Rahmati-Yamchi

DOI: https://dx.doi.org/10.12692/ijb/4.10.134-145

Int. J. Biosci. 4(10), 134-145. (PDF)

Abstract:

lung cancer is the most common cancer in men still now. Telomerase is responsible for cancerous cells immortality and is a suitable target for cancer therapy. TRF1 is a modulator for telomerase activity. It is necessary to find more efficient and safer anticancer drugs. Curcumin is a natural polyphenol which has many anticancer effects but it has hydrophobic structure and low solubility in water. PLGA-PEG nanoparticles was used to comprise effects of free curcumin and curcumin loaded PLGA-PEG on telomerase and TRF1 expressions in lung cancer cell line. 1H NMR, FT-IR and SEM confirmed PLGA-PEG structure and curcumin loading on it. Then, cytotoxic effects of free curcumin and curcumin loaded PLGA-PEG determined by MTT assay. mRNA expression levels of hTERT and TRF1 was determined by Real-time PCR. MTT assay data analysis indicated that curcumin cytotoxicity is dose and time-dependent. Curcumin loaded nanoparticles showed IC50 values in lower concentration in comparison to free curcumin. Curcumin loaded PLGA-PEG decreased hTERT expression and increased TRF1 expression more than pure curcumin. Our study demonstrates curcumin loaded PLGA-PEG promises a natural and efficient system for anticancer drug delivery to fight lung cancer.