The effect of 2,4-D on callus induction using leaf lobe of sweet potato as a source of explant
Paper Details
The effect of 2,4-D on callus induction using leaf lobe of sweet potato as a source of explant
Abstract
The effect of 2, 4-dichlorophenoxyacetic acid (2,4-D) on young leaf lobes of three sweet potato accessions UE007, UK-BNARI and SA-BNARI for callus induction was investigated. Callusogenesis was achieved when leaf lobe explants from four weeks old healthy growing plantlets of three sweet potato accessions SA-BNARI, UK-BNARI and UE007 were cultured on CLC/ Ipomoea medium supplemented with 1.0 – 4.0 mg/l 2,4-D with 4.0 mg/l 2,4-D being the optimal concentration. However, the calli were non-embryogenic and therefore could not produce embryos when transferred to 0.1 mg/l BAP amended medium but rather produced either single or multiple shoots. The highest percentage shoot (83.3 %) was obtained from 4.0 mg/l 2,4-D-derived callus. These results indicate that indirect shoot development via a callus phase is a feasible option for sweet potato propagation using in vitro techniques.
Akoroda M. 2009. Sweet potato in West Africa. In: G. Loebenstein and G. Thotthappilly, (eds). The sweet potato. Springer Sciences Business Media BV, Dordrecht, The Netherlands. 441–468.
Ali A, Naz S, Iqbal J. 2007.Effect of different explants and media compositions for efficient somatic embryogenesis in sugarcane (Saccaharumofficinarum). Pakistan Journal of Botany 39(6), 1961-1977.
Badoni A, Chauhan JS. 2010. Conventional vis -a-vis Biotechnological Methods of Propagation in Potato. Stem Cell 1, 1-6.
Barka GD. 2009. In vitro Production of Virus Free Sweet Potato [Ipomoea batatas(L.) Lam] by Meristem Culture and Thermotherapy.MSc. Dissertation, Addis Ababa University, Addis Ababa Ethiopia.
Chee YK, Hacker JB, Ramirez L, Chen C P. 1992. Canavalia ensiformis (L.) DC.. Record from Proseabase. Mannetje, L.’t and Jones, R.M. (Editors). PROSEA (Plant Resources of South-East Asia) Foundation, Bogor, Indonesia.
Chugh A, Khurana P. 2002. Gene expression during somatic embryogenesis – recent advances.Current Science 83(6), 715-739.
Danso KE, Acheampong E, Amoatey HM. 1999. Selection andin-vitro propagation of five cassava (Manihotesculenta, Crantz) cultivars. Journal ofthe Ghana Science Association 1(3), 31-41.
El Far MM., El Mangoury K, Elazab HEM. 2009. Novel plant regeneration for Egyptian sweet potato (Ipomoea batatas(L.) Lam.) cv. abeesvia indirect organogenesis stimulated by initiation medium and cytokinin effects.Australian Journal of Basic and Applied Sciences 3(2), 543-551.
Feher A, Pasternak T, Dudits D. 2003. Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue and Organ Culture 74(3), 201-228.
Kobayashi M, Akita S. 1966. Studies on the breeding of sweet potato adapted for seed-tuber planting culture. I. variations of yielding ability of F1 plants. (in Japanese with English summary). Japanese Journal of Breeding15, 57.
KumariJayasree P, Asokan MP, Sobha S, SankariAmmal L, Rekha K, Kala RG, Jayasree R, Thulaseedharan A. 1999. Somatic embryogenesis and Plant regeneration from immature anthers of Heveabrasiliens (Muell. Arg). Current Science 76, 1242-1245.
Liu QC, Mi KX, Lu DH, Zhovard YH, Fu Z. 1997. Establishment of embryogenic cell, Ipomoea batatas (L) Lam. Acta Agriculturae Boreali Sinica 23(1), 22-26.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Journalof PlantPhysiology 15, 473–497.
Newell CA, Lowe JA, Merryweather A, Rooke LM, Hamilton WDO. 1995. Transformation Sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacteriumtumefaciens. Journal of Plant Science 107, 215-227.
Oggema JN, Kinyua MG, Ouma JP, Owuoche J O. 2007. Agronomic performance of locally adapted sweet potato (Ipomoea batatas(L) Lam.) cultivars derived from tissue culture regenerated plants. African Journal of Biotechnology 6(12), 1418-1425.
Otani M, Wakita Y, Shimada T. 2003. Production of herbicide resistant sweet potato (Ipomoea batatas L. Lam.) plants by Agrobacterium tumefaciens- mediated transformation. Breed Science 53, 145-148.
Soomro R, Yasmin S, Markhand GS, Ahmed B, Mahar AQ. 1993. Induction and growth of callus derived from Vigna radiate L., hypocotyl explants, Scientific Sindh. Annual Journal Research 1, 159-166.
F. Addae-Frimpomaah, E. Arkorful, T.K. Tengey , 2014. The effect of 2,4-D on callus induction using leaf lobe of sweet potato as a source of explant. Int. J. Agron. Agric. Res., 5(1), 16-22.
Copyright © 2014 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.