The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat
Paper Details
The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat
Abstract
Moringa leaves contain various nutrients that can protect spermatozoa during semen cryopreservation. This study aimed to examine the effect of moringa leaf extract in tris extender on the quality of frozen semen of Boer crossbreed goat. Semen was collected with an artificial vagina. Fresh semen was divided into four tubes and diluted with 73% tris extender + 20% egg yolk + 7% glycerol (control), 71% tris extender + 20% egg yolk + 7% glycerol + 2% moringa leaf extract (MLE-2), 69% tris extender + 20% egg yolk + 7% glycerol + 4% moringa leaf extract (MLE-4), and 67% tris extender + 20% egg yolk + 7% glycerol + 6% moringa leaf extract (MLE-6), respectively. Diluted-semen was loaded in a mini straw and then stored in a liquid nitrogen container for seven days. The quality of spermatozoa including motility, viability, and intact plasma membrane (IPM) were observed after diluting and thawing. The result showed that the addition of moringa leaf extract into the tris extender could improve the spermatozoa quality of frozen semen of Boer crossbreed goat. At the stage after thawing, the percentage of motility, percentage of viability, and percentage of IPM of MLE-4 (52.2, 65.2, and 65.0%) were significantly higher (p<0.05) compared to the control (43.7, 53.5, and 53.7%). It can be concluded that the addition of 4% moringa leaf extract is the appropriate concentration in producing frozen semen of Boer crossbreed.
Bearden HJ, Fuquay JW. 2000. Applied Animal Reproduction 5th Ed. Mississippi State University, New Jersey.
Cahyadi TRT, Christiyanto M, Setiatin ET. 2016. Persentase hidup dan abnormalitas sel spermatozoa kambing Peranakan Ettawa (PE) dengan pakan yang disuplementasikan daun binahong (Anredera cordifolia (Ten) steenis). Animal Agriculture Journal 5, 23-32.
Das AK, Rajkumar V, Verma AK, Swarup D. 2012. Moringa oleifera leaves extract: a natural antioxidant for retarding lipid peroxidation in cooked goat meat patties. International Journal of Food Science and Technology 47, 585–591. https://doi.org/10.1111/j.1365-2621.2011.02881.x
Fafo M, Hine TM, Nalley WMM. 2016. Pengujian efektivitas ekstrak daun kelor dalam pengencer sitrat kuning telur terhadap kualitas semen cair babi landrace. Jurnal Nukleus Peternakan 3, 184-195.
Felipe-Perez YE, Juarez-Mosqueda ML, Hernandez-Gonzalez EO, Valencia JJ. 2008. Viability of fresh and frozen bull sperm compared by two staining techniques. Acta Veterinaria Brasasilica 2, 123-130. http://dx.doi.org/10.21708/avb.2008.2.4.895
Gopalakrishnan L, Doriya K, Kumar DS. 2016. Moringa oleifera: a review on nutritive importance and its medicinal application. Journal of Food Science and Human Wellness 5, 49-56. https://doi.org/10.1016/j.fshw.2016.04.001
Kasolo JN, Bimeya GS, Ojok L, Ochieng J, Okwal-okeng JW. 2010. Phytochemicals and uses of Moringa oleifera leaves in Uganda rural communities. Journal of Medical Plant Research 4, 753-757. https://doi.org/10.5897/JMPR10.492
Kumala IN, Masfufatun, Devi DRE. 2016. Potensi ekstrak daun kelor (Moringa oleifera) sebagai hepatoprotektor pada tikus putih (Rattus novergicus) yang diinduksi parasetamol dosis toksis. Jurnal Ilmiah Kedokteran 5, 58-66. http://dx.doi.org/10.30742/jikw.v5i1.6
Rasul Z, Ahmad N, Anzar M. 2001. Changes in motion characteristics, plasma membrane integrity and acrosome morphology during cryopreservation of buffalo spermatozoa. Journal of Andrology 22, 278-283.
Revell SG, Mrode RA. 1994. An osmotic resistance test for bovine semen. Animal Reproduction Science 36, 77-86. https://doi.org/10.1016/0378-4320(94)90055-8
Rizal M, Riyadhi M, Sulaiman A. 2018. The quality of Boer goat semen preserved with sugar palm juice. Bulletin of Animal Science 42, 97-102. https://doi.org/10.21059/buletinpeternak.v42i2.28236
Salmani H, Towhidi A, Zhandi M, Bahreini M, Sharafi M. 2014. In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology 68, 276-280. https://doi.org/10.1016/j.cryobiol.2014.02.008
Sitepu SA, Udin Z, Jaswandi, Hendri. 2018. Quality differences of Boer liquid semen during storage with addition sweet orange essential oil in tris yolk and gentamicin extender. Journal of Community Service and Research 1, 78-82. https://doi.org/10.24114/jcrs.v1i2.9341
Standar Nasional Indonesia (SNI). 2014. Semen Beku Bagian 3: Kambing dan Domba (SNI 4869.3-2014). Badan Standardisasi Nasional, Jakarta.
Steel RGD, Torrie JH. 1993. Prinsip dan Prosedur Statistika. Gramedia Pustaka Utama, Jakarta.
Sokunbi OA, Ajani OS, Lawanson AA, Amao EA. 2015. Antibiotic potential of moringa leaf (Moringa oleifera Lam) crude extract in bull semen extander. European Journal of Medicinal Plants 9, 1-8. https://doi.org/10.9734/EJMP/2015/18546
Tamoes JA, Nalley WMM, Hine TM. 2014. Fertilitas spermatozoa babi Landrace dalam pengencer modifikasi zorlesco dengan susu kacang kedelai. Sains Peternakan 12, 20-30. https://doi.org/10.20961/sainspet.v12i1.4772
Muhammad Riyadhi, Nursyam Andi Syarifuddin, Muhammad Thahir, Muhammad Rizal, 2022. The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat. Int. J. Biosci., 21(5), 77-82.
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