The effect of cold stress on H2O2 and MDA contents in barely genotypes

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Research Paper 01/09/2015
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The effect of cold stress on H2O2 and MDA contents in barely genotypes

Rana Valizadeh Kamran, Mahmoud Toorchi, Mohammad Moghadam, Hamid Mohammadi
J. Bio. Env. Sci.7( 3), 66-75, September 2015.
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During the course of cold acclimation, a key process associated with plant cold tolerance, some biochemical components may be up- or down-regulated. In carrying out this experiment, the objective was to investigate the correlation between acclimation and hydrogen peroxide (H2O2) and malondialdehyde (MDA) production under monitored conditions (regular chamber and cold regime) in 20 barely genotypes through in a split plot experiment, with temperatures as main plot and genotypes as a sub plot, based on a complete randomized blocks design with three replicates. According to the results, there was a significant difference in the amount of H2O2 and MDA between the two temperature conditions, so was the difference between genotypes regarding MDA content. However, no significant difference was observed between genotypes concerning H2O2 content. Further analysis of variance, for MDA content before and after acclimation as well as discrepancies between the two conditions, was conducted using a complete randomized block design, which robustly discriminated the genotypes performance after cold acclimation, and revealed the significant difference between the two temperature conditions. Some genotypes (EC83-12 and Schulyer) possessed the least amount of H2O2 and MDA, hence designated as tolerant, and genotypes (Kavir and Aths) with highest amount of H2O2 and MDA were considered cold sensitive. There also existed a positive, significant correlation between MDA and H2O2 contents in the event of difference between control and post- acclimation treatment. Finally, cluster analysis of the mean contents of MDA and H2O2 in genotypes, using “linkage between groups” method, resulted in four groups as sensitive, semi-sensitive, semi-tolerant and tolerant. Present study has been planned to examine the role of MDA and H2O2 related to cold stress of tolerant and sensitive barley.


Apostolova P, Yordanova R, Popova L. 2008. Response of antioxidative defence system to low temperature stress in two wheat cultivars. General and Applied Plant Physiology 34, 281- 294.

Baek KH, Skinner DZ. 2012. Production of reactive oxygen species by freezing stress and the protective roles of antioxidant enzymes in plants, Journal of Agricultural Chemistry and Environment 1, 34- 40.

Blokhina O, Virolainen E, Fagerstedt KV. 2003.  Antioxidants, oxidative damage  and  oxygen deprivation stress. A review. Annals of Botany 91, 179-194.

Desikan R, Hancock JT, Neil SJ. 2004. Oxidative stress signaling. Plants response to abiotic stress 4, 120- 150.

Dhindsa RS. 1991. Drought stress, enzymes of glutathione metabolism, oxidation injury, and protein synthesis in Tortula ruralis. Plant Physiology 95, 648- 651.

Fahimirad S, Karimzadeh G, Ghanati F. 2013. Cold- induced changes of Antioxidant Enzymes Activity and Lipid peroxidation in two canola cultivars. Journal of Plant Physiology and Breeding 3(1), 1-11.

Fowler DB, Dvorak J, Gusta LV. 1977. Comparative cold hardiness of several Triticum species and Secale cereale L. Crop Science 17, 941-943.

Gadjev I, Stone JM, Gechev TS. 2008. Programmed Cell Death in Plants: New Insights into Redox Regulation and the Role of Hydrogen Peroxide. International Review of Cell and Molecular 270, 87-144.

Gong H, Zhu X, Chen K, Wang S, and Zhang C. 2005. Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science 169, 313-321.

Guo Z, Ou W, Lu S, Zhong Q. 2006. Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry 44, 828- 836.

Halliwell B. 2006. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiology 141, 312– 322.

Huang M, Guo Z. 2005. Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity. Biologia Plantarum 49(1), 81-84.

Knight H, Knight MR. 2001. Abiotic stress signalling pathways: specificity and cross-talk. Trends in plant science 6, 262- 267.

Kóti K, Karsai I, Szűcs P, Horváth C, Mészáros K, Kiss GB, Hayes PM. 2006. Validation of the two-gene epistatic model for vernalization response in a winter× spring barley cross. Euphytica 152, 17-24.

Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F. 1999. Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiology 119, 1091-1100.

McKersie BD, Murnaghan J, Jones KS, Bowley SR. 2000. Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiology 122, 1427- 37.

Ma NL, Rahmat Z, Lam SS. 2013. A Review of the “Omics” Approach to Biomarkers of Oxidative Stress in Oryza sativa, International Journal of Molecular Sciences 14, 7515- 7541.

Öktem HA, Eyidoðan F, Demirba D, Bayraç AT, Öz MT, Özgür E, Yücel M. 2008. Antioxidant responses of lentil to cold and drought stress. Journal of Plant Biochemistry and Biotechnology 17, 15- 21.

Palva ET, Welling A, Tähtiharju S, Tamminen I, Puhakainen T, Mäkelä P, Laitinen R. 2001. Cold acclimation and development of freezing and drought tolerance in plants. Acta Horticultureae 560, 277- 284.

Ramankutty N, Evan AT, Monfreda C, Foley JA. 2008. Farming the planet: 1. Geographic distribution of global agricultural lands in the year 2000. Global Biogeochemical Cycles 22, 1.

Sairam RK, Chandrasekhar V, Srivastava GC. 2001. Comparison of hexaploid and tetraploid wheat cultivars in their responses to water stress. Biologia Plantarum 44, 89- 94.

Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101, 7-12.

Sleper DA, Poehlman JM. 2006. Breeding field crops. Blackwell publishing. In: Thomashow MF , Ed. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annual review of plant biology 50, 571- 599.

Stepien P, Klobus G. 2005. Antioxidant defense in the leaves of C3 and C4 plants under salinity stress. Physiologia Plantarum 125, 31-40.

Stewart RR, Bewley JD. 1980. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology 65(2), 245-248.

Vaidyanathan H, Sivakumar P, Chakrabarsty R, Thomas G. 2003. Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) differential response in salt tolerant and sensitive varieties. Plant Science 165, 1411-1418.

Wang WB, Kim YH, Lee HS, Yong KK, Deng X, Kwak S. 2009. Analysis of antioxidant ezyme activity during germination of alfalfa under salt and drought stresses. Journal of Plant Physiology and Biochemistry 47, 570-577.

Warmund MR, Guinan P, Fernandez G. 2008. Temperatures and cold damage to small fruit crops across the eastern United States associated with the April 2007 freeze. HortScience 43, 1643-1647.

Yamazaki J, Ohashi A, Hashimoto Y, Negishi E, Kumagai S, Kubo T, Oikawa T, Maruta E, Kamimura Y. 2003. Effects of high light and low temperature   during   harsh   winter   on   needle photodamage of Abies mariesii growing at the forest limit on Mt. Norikura in Central Japan. Plant Science165, 257-264.

Zhang Y, Liu C, Shen Y, Kondoh A, Tang C, Tanaka  T,  Shimada  J.  2002.  Measurement  of evapotranspiration   in   a   winter   wheat   field. Hydrological processes 16, 2805- 2817.